Effect of extremely low frequency electromagnetic field on MAP2 and Nestin gene expression of hair follicle dermal papilla cells.

The International journal of artificial organs · 2016 · Mixed · Very low evidence

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MixedVery low evidencePaper 4832

Cells exposed to ELF-EMF (50 Hz, 1 mT) showed higher MAP2 expression after 14 days compared with 5 days in the EMF group. A decrease in cell proliferation from days 5 to 20 was reported, interpreted as consistent with a positive effect of extended treatment t…

Study snapshot

PublicationThe International journal of artificial organs · 2016

Study typein_vitro

Reported directionMixed

Evidence ratingVery low

Abstract

INTRODUCTION: In recent years, the extremely low frequency electromagnetic field (ELF-EMF) has attracted a great deal of scientific interest. The ELF-EMF signal is able to control ion transport across ion channels and therefore induce cell differentiation. AIM: The purpose of this study was to investigate the effect of ELF-EMF (50 Hz, 1 mT) on MAP2 and Nestin gene expression of dermal papilla mesenchymal cells (DPCs). METHODS: In order to examine the effect of chemical and electromagnetic factors on gene expression, 4 experimental groups, namely chemical (cell exposure to chemical signals), EMF (exposing cells to ELF-EMF), chemical-EMF (subjecting cells to chemical signals and ELF-EMF) and control (with no treatment) groups, were prepared, treated for 5 days, and studied. To assess the effect of extended test time on the expression of neural differentiation markers (Nestin and MAP2), an EMF group was prepared and treated for a period of 14 consecutive days. The beneficial role of EMF in inducing neural differentiation was shown by real-time PCR analysis. RESULTS: The higher expression of MAP2 after 14 days compared to that after 5 days and decrease of cell proliferation on days 5 to 20 were indicative of the positive effect of extending treatment time on neural differentiation by evaluation of gene expression in EMF group.

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AI evidence extraction

Structured fields below were generated from the paper’s abstract and metadata to make the evidence easier to inspect. Automated extraction may be incomplete or incorrect; verify important details against the original paper.

Study typein_vitro

Effect directionmixed

PopulationHair follicle dermal papilla mesenchymal cells (dermal papilla cells, DPCs)

Sample sizeNot supplied

ExposureELF · 5e-05 MHz · 5 days; 14 consecutive days (EMF group)

Evidence strengthVery lowConfidence: 74% · Peer reviewed: yes

Main findings

Outcomes measured

MAP2 gene expression
Nestin gene expression
cell proliferation

Limitations

In vitro study; findings may not generalize to humans or in vivo conditions
Sample size not reported in the abstract
Details of exposure regimen (daily exposure duration, waveform) not provided in the abstract
Results for Nestin and for the chemical and chemical-EMF groups are not reported in the abstract

View raw extracted JSON

{
  "study_type": "in_vitro",
  "exposure": {
    "band": "ELF",
    "source": "other",
    "frequency_mhz": 5e-05,
    "sar_wkg": null,
    "duration": "5 days; 14 consecutive days (EMF group)"
  },
  "population": "Hair follicle dermal papilla mesenchymal cells (dermal papilla cells, DPCs)",
  "sample_size": null,
  "outcomes": [
    "MAP2 gene expression",
    "Nestin gene expression",
    "cell proliferation"
  ],
  "main_findings": "Cells exposed to ELF-EMF (50 Hz, 1 mT) showed higher MAP2 expression after 14 days compared with 5 days in the EMF group. A decrease in cell proliferation from days 5 to 20 was reported, interpreted as consistent with a positive effect of extended treatment time on neural differentiation based on gene expression evaluation.",
  "effect_direction": "mixed",
  "limitations": [
    "In vitro study; findings may not generalize to humans or in vivo conditions",
    "Sample size not reported in the abstract",
    "Details of exposure regimen (daily exposure duration, waveform) not provided in the abstract",
    "Results for Nestin and for the chemical and chemical-EMF groups are not reported in the abstract"
  ],
  "evidence_strength": "very_low",
  "confidence": 0.74,
  "peer_reviewed_likely": "yes",
  "keywords": [
    "extremely low frequency electromagnetic field",
    "ELF-EMF",
    "50 Hz",
    "1 mT",
    "dermal papilla cells",
    "hair follicle",
    "MAP2",
    "Nestin",
    "gene expression",
    "neural differentiation",
    "cell proliferation",
    "real-time PCR"
  ],
  "suggested_hubs": [
    {
      "slug": "occupational-exposure",
      "weight": 0.2,
      "reason": "Study involves ELF exposure parameters (50 Hz) relevant to some occupational settings, though this is an in vitro experiment and no workplace context is described."
    }
  ]
}

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AI-extracted fields are generated from the abstract and metadata and may be incomplete or incorrect. This material is for informational purposes and is not medical advice.

Identifiers and source

DOI: 10.5301/ijao.5000512
PubMed: 27515859

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