Kinetic study on the effects of extremely low frequency electromagnetic field on catalase, cytochrome P450 and inducible nitric oxide synthase in human HaCaT and THP-1 cell lines.

CNS & neurological disorders drug targets · 2011 · Unclear · Insufficient evidence

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UnclearInsufficient evidencePaper 4423

Study snapshot

PublicationCNS & neurological disorders drug targets · 2011

Study typein_vitro

Reported directionUnclear

Evidence ratingInsufficient

Abstract

Extremely low frequency electromagnetic fields (ELF-EMF) have been found to produce a variety of biological effects. These effects of ELF-EMF depend upon frequency, amplitude, and length of exposure, and are also related to intrinsic susceptibility and responsiveness of different cell types. Although the mechanism of this interaction is still obscure, ELF-EMF can influence cell proliferation, differentiation, cell cycle, apoptosis, DNA replication and protein expression. The aim of this study was to estimate various kinetic constants of catalase, cytochrome P450 and inducible nitric oxide synthase in response to ELF-EMF exposure in human HaCaT and THP-1 cell lines. In order to evaluate the effect of ELF-EMF on the modulation of cellular responses to an inflammatory stimulus, both cell lines were treated with lipopolysaccharide. To the best of our knowledge there is no available report on such type of kinetic study of selected enzymes in response to ELF-EMF in these cell lines. Therefore, the current study may reveal novel mechanism of ELFEMF biological interaction with the enzymological and hormonal systems of living organisms. These new insights may be important for ELF-EMF application particularly for wound healing, tissue regeneration, Parkinson's and Alzheimer's diseases.

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AI evidence extraction

Structured fields below were generated from the paper’s abstract and metadata to make the evidence easier to inspect. Automated extraction may be incomplete or incorrect; verify important details against the original paper.

Study typein_vitro

Effect directionunclear

PopulationHuman HaCaT and THP-1 cell lines

Sample sizeNot supplied

ExposureELF

Evidence strengthInsufficientConfidence: 62% · Peer reviewed: yes

Main findings

Outcomes measured

Kinetic constants of catalase
Kinetic constants of cytochrome P450
Kinetic constants of inducible nitric oxide synthase (iNOS)
Modulation of cellular responses to inflammatory stimulus (lipopolysaccharide-treated cells)

Limitations

No exposure parameters reported in abstract (e.g., frequency, amplitude, duration).
No results or quantitative findings reported in abstract.

View raw extracted JSON

{
  "study_type": "in_vitro",
  "exposure": {
    "band": "ELF",
    "source": null,
    "frequency_mhz": null,
    "sar_wkg": null,
    "duration": null
  },
  "population": "Human HaCaT and THP-1 cell lines",
  "sample_size": null,
  "outcomes": [
    "Kinetic constants of catalase",
    "Kinetic constants of cytochrome P450",
    "Kinetic constants of inducible nitric oxide synthase (iNOS)",
    "Modulation of cellular responses to inflammatory stimulus (lipopolysaccharide-treated cells)"
  ],
  "main_findings": null,
  "effect_direction": "unclear",
  "limitations": [
    "No exposure parameters reported in abstract (e.g., frequency, amplitude, duration).",
    "No results or quantitative findings reported in abstract."
  ],
  "evidence_strength": "insufficient",
  "confidence": 0.62,
  "peer_reviewed_likely": "yes",
  "keywords": [
    "extremely low frequency electromagnetic field",
    "ELF-EMF",
    "catalase",
    "cytochrome P450",
    "inducible nitric oxide synthase",
    "iNOS",
    "HaCaT",
    "THP-1",
    "lipopolysaccharide",
    "in vitro",
    "enzyme kinetics",
    "inflammation"
  ],
  "suggested_hubs": []
}

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AI-extracted fields are generated from the abstract and metadata and may be incomplete or incorrect. This material is for informational purposes and is not medical advice.

Identifiers and source

DOI: 10.2174/187152711799219325
PubMed: 22229327

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