The effect of electromagnetic field on reactive oxygen species production in human neutrophils in vitro.

Electromagnetic biology and medicine · 2013 · Mixed · Low evidence

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MixedLow evidencePaper 4493

Only EMF tuned to the calcium ion cyclotron resonance frequency affected ROS production in neutrophils. The effect depended on magnetic induction (10, 40, 60 μT): EMF slightly inhibited activity in unstimulated neutrophils, while PMA-stimulated neutrophils sh…

Study snapshot

PublicationElectromagnetic biology and medicine · 2013

Study typein_vitro

Reported directionMixed

Evidence ratingLow

Abstract

The present study was undertaken in order to determine the effect of low frequency electromagnetic field (EMF) on reactive oxygen species (ROS) production in human neutrophils in peripheral blood in vitro. We investigated how differently generated EMF and several levels of magnetic induction affect ROS production. To evaluate the level of ROS production, two fluorescent dyes were used: 2'7'-dichlorofluorscein-diacetate and dihydrorhodamine. Phorbol 12-myristate 13-acetate (PMA), known as strong stimulator of the respiratory burst, was also used. Alternating magnetic field was generated by means of Viofor JPS apparatus. Three different levels of magnetic induction have been analyzed (10, 40 and 60 μT). Fluorescence of dichlorofluorescein and 123 rhodamine was measured by flow cytometry. The experiments demonstrated that only EMF tuned to the calcium ion cyclotron resonance frequency was able to affect ROS production in neutrophils. Statistical analysis showed that this effect depended on magnetic induction value of applied EMF. Incubation in EMF inhibited cell activity slightly in unstimulated neutrophils, whereas the activity of PMA-stimulated neutrophils has increased after incubation in EMF.

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AI evidence extraction

Structured fields below were generated from the paper’s abstract and metadata to make the evidence easier to inspect. Automated extraction may be incomplete or incorrect; verify important details against the original paper.

Study typein_vitro

Effect directionmixed

PopulationHuman neutrophils from peripheral blood (in vitro)

Sample sizeNot supplied

ExposureELF

Evidence strengthLowConfidence: 74% · Peer reviewed: yes

Main findings

Outcomes measured

Reactive oxygen species (ROS) production in neutrophils
Fluorescence of dichlorofluorescein (DCF)
Fluorescence of rhodamine 123 (from dihydrorhodamine)

Limitations

In vitro study using isolated human neutrophils; generalizability to in vivo health outcomes is unclear
Exposure frequency and exposure duration not reported in the abstract
Sample size not reported in the abstract
Effect observed only for EMF tuned to calcium ion cyclotron resonance frequency; details of tuning not provided in the abstract

View raw extracted JSON

{
  "study_type": "in_vitro",
  "exposure": {
    "band": "ELF",
    "source": "other",
    "frequency_mhz": null,
    "sar_wkg": null,
    "duration": null
  },
  "population": "Human neutrophils from peripheral blood (in vitro)",
  "sample_size": null,
  "outcomes": [
    "Reactive oxygen species (ROS) production in neutrophils",
    "Fluorescence of dichlorofluorescein (DCF)",
    "Fluorescence of rhodamine 123 (from dihydrorhodamine)"
  ],
  "main_findings": "Only EMF tuned to the calcium ion cyclotron resonance frequency affected ROS production in neutrophils. The effect depended on magnetic induction (10, 40, 60 μT): EMF slightly inhibited activity in unstimulated neutrophils, while PMA-stimulated neutrophils showed increased activity after EMF incubation.",
  "effect_direction": "mixed",
  "limitations": [
    "In vitro study using isolated human neutrophils; generalizability to in vivo health outcomes is unclear",
    "Exposure frequency and exposure duration not reported in the abstract",
    "Sample size not reported in the abstract",
    "Effect observed only for EMF tuned to calcium ion cyclotron resonance frequency; details of tuning not provided in the abstract"
  ],
  "evidence_strength": "low",
  "confidence": 0.74,
  "peer_reviewed_likely": "yes",
  "keywords": [
    "electromagnetic field",
    "low frequency",
    "ELF",
    "alternating magnetic field",
    "magnetic induction",
    "microtesla",
    "reactive oxygen species",
    "ROS",
    "neutrophils",
    "oxidative burst",
    "PMA",
    "flow cytometry",
    "dichlorofluorescein-diacetate",
    "dihydrorhodamine",
    "calcium ion cyclotron resonance",
    "Viofor JPS"
  ],
  "suggested_hubs": []
}

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AI-extracted fields are generated from the abstract and metadata and may be incomplete or incorrect. This material is for informational purposes and is not medical advice.

Identifiers and source

DOI: 10.3109/15368378.2012.721845
PubMed: 23137127

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