Static magnetic fields generated by a 0.5 T MRI unit affects in vitro expression of activation markers and interleukin release in human peripheral blood mononuclear cells (PBMC).

International journal of radiation biology · 1999 · Mixed · Low evidence

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MixedLow evidencePaper 4037

After 24 h of culture following exposure, the 0.5 T static magnetic field reduced CD69 expression in PBMC in vitro; this reduction was enhanced after PHA stimulation. IFN-gamma and IL-4 release increased, but these increases were reduced after PHA stimulation…

Study snapshot

PublicationInternational journal of radiation biology · 1999

Study typein_vitro

Reported directionMixed

Evidence ratingLow

Abstract

PURPOSE: To investigate the effects of the static magnetic field (SMF) generated by a 0.5 T superconducting MRI unit on in vitro activation marker expression and interleukin release in human peripheral blood mononuclear cell (PBMC) samples from healthy volunteers. MATERIALS AND METHODS: PBMC samples were split into two groups: exposed and sham-exposed under isothermal conditions. PBMC were exposed for 2 h at 24 degrees C to the SMF of a 0.5 T superconducting MRI unit. Immediately after exposure, both samples were cultured for 24 h at 37 degrees C with or without mitogenic stimulation by phytohaemagglutinin (PHA). PBMC were examined for expression of CD25, CD69 and CD71 by immunofluorescence analysis and supernatants were assayed to quantify IFN-gamma, TNF-alpha and IL-4 by ELISA. RESULTS: The 0.5 T SMF produced, after 24 h of culture, a reduced expression of CD69 from PBMC in vitro, that was enhanced after PHA stimulation. An increased release of IFN-gamma and IL-4 was also found, which was reduced after PHA stimulation. The release of TNF-alpha, IL-6 and IL-10 was not modified. CONCLUSIONS: The SMF generated by a 0.5 T superconducting MRI unit modified in vitro activation marker expression and interleukin release from human PBMC.

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AI evidence extraction

Structured fields below were generated from the paper’s abstract and metadata to make the evidence easier to inspect. Automated extraction may be incomplete or incorrect; verify important details against the original paper.

Study typein_vitro

Effect directionmixed

PopulationHuman peripheral blood mononuclear cells (PBMC) from healthy volunteers (in vitro)

Sample sizeNot supplied

Exposurestatic · 2 h exposure at 24°C (then 24 h culture at 37°C with/without PHA)

Evidence strengthLowConfidence: 78% · Peer reviewed: yes

Main findings

Outcomes measured

PBMC activation marker expression (CD25, CD69, CD71)
Interleukin/cytokine release (IFN-gamma, TNF-alpha, IL-4; also mentions IL-6, IL-10)

Limitations

Sample size not reported in abstract
In vitro study; findings may not generalize to in vivo human health outcomes
Exposure conditions limited to 0.5 T static field and a single 2 h exposure duration

View raw extracted JSON

{
  "study_type": "in_vitro",
  "exposure": {
    "band": "static",
    "source": "MRI unit",
    "frequency_mhz": null,
    "sar_wkg": null,
    "duration": "2 h exposure at 24°C (then 24 h culture at 37°C with/without PHA)"
  },
  "population": "Human peripheral blood mononuclear cells (PBMC) from healthy volunteers (in vitro)",
  "sample_size": null,
  "outcomes": [
    "PBMC activation marker expression (CD25, CD69, CD71)",
    "Interleukin/cytokine release (IFN-gamma, TNF-alpha, IL-4; also mentions IL-6, IL-10)"
  ],
  "main_findings": "After 24 h of culture following exposure, the 0.5 T static magnetic field reduced CD69 expression in PBMC in vitro; this reduction was enhanced after PHA stimulation. IFN-gamma and IL-4 release increased, but these increases were reduced after PHA stimulation. TNF-alpha, IL-6 and IL-10 release were not modified.",
  "effect_direction": "mixed",
  "limitations": [
    "Sample size not reported in abstract",
    "In vitro study; findings may not generalize to in vivo human health outcomes",
    "Exposure conditions limited to 0.5 T static field and a single 2 h exposure duration"
  ],
  "evidence_strength": "low",
  "confidence": 0.78,
  "peer_reviewed_likely": "yes",
  "keywords": [
    "static magnetic field",
    "MRI",
    "0.5 T",
    "PBMC",
    "CD69",
    "CD25",
    "CD71",
    "IFN-gamma",
    "IL-4",
    "TNF-alpha",
    "IL-6",
    "IL-10",
    "phytohaemagglutinin",
    "in vitro"
  ],
  "suggested_hubs": [
    {
      "slug": "mri-exposure",
      "weight": 0.9,
      "reason": "Study examines static magnetic field exposure from a 0.5 T MRI unit."
    }
  ]
}

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AI-extracted fields are generated from the abstract and metadata and may be incomplete or incorrect. This material is for informational purposes and is not medical advice.

Identifiers and source

DOI: 10.1080/095530099140384
PubMed: 10331851

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