Exposure to strong magnetic fields at power frequency potentiates X-ray-induced DNA strand breaks.

Journal of radiation research · 2000 · Mixed · Low evidence

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MixedLow evidencePaper 3977

Study snapshot

PublicationJournal of radiation research · 2000

Study typein_vitro

Reported directionMixed

Evidence ratingLow

Abstract

We examined the effect of an extremely low-frequency magnetic field (ELFMF) at 5, 50 and 400 mT on DNA strand breaks in human glioma MO54 cells. A DNA damage analysis was performed using the method of alkaline comet assay. The cells were exposed to X-rays alone (5 Gy), ELFMF alone, or X-rays followed by ELFMF at 4 degrees C or on ice. No significant difference in the tail moment was observed between control and ELFMF exposures up to 400 mT. X-ray irradiation increased DNA strand breaks. When cells were exposed to X-rays followed by ELFMF at 50 and 400 mT, the tail moment increased significantly compared with that for X-rays alone. When the exposure of cells was performed at 37 degrees C, no significant change was observed between X-rays alone and X-rays plus 400 mT. We previously observed that exposure to 400 mT ELFMF for 2 h increased X-ray-induced mutations (Miyakoshi et al, Mutat. Res., 349: 109-114, 1996). Additionally, an increase in the mutation by exposure to the ELFMF was observed in cells during DNA-synthesizing phase (Miyakoshi et al., Int. J. Radiat. Biol., 71: 75-79, 1997). From these results, it appears that exposure to the high density ELFMF at more than 50 mT may potentiate X-ray-induced DNA strand breaks.

Machine Enhanced Logic

AI evidence extraction

Structured fields below were generated from the paper’s abstract and metadata to make the evidence easier to inspect. Automated extraction may be incomplete or incorrect; verify important details against the original paper.

Study typein_vitro

Effect directionmixed

PopulationHuman glioma MO54 cells

Sample sizeNot supplied

ExposureELF

Evidence strengthLowConfidence: 74% · Peer reviewed: yes

Main findings

In MO54 cells, ELFMF exposure alone (5, 50, 400 mT) did not significantly change comet assay tail moment versus control up to 400 mT. X-rays (5 Gy) increased DNA strand breaks, and subsequent ELFMF exposure at 50 and 400 mT (at 4°C/on ice) significantly increased tail moment compared with X-rays alone; at 37°C, no significant difference was observed between X-rays alone and X-rays plus 400 mT.

Outcomes measured

DNA strand breaks (alkaline comet assay tail moment)

Limitations

In vitro cell model (human glioma MO54 cells)
Exposure duration not reported in the abstract
Power frequency value not specified in the abstract
Sample size and replication details not reported in the abstract
Effect depended on temperature/exposure conditions (4°C/on ice vs 37°C)

View raw extracted JSON

{
  "study_type": "in_vitro",
  "exposure": {
    "band": "ELF",
    "source": null,
    "frequency_mhz": null,
    "sar_wkg": null,
    "duration": null
  },
  "population": "Human glioma MO54 cells",
  "sample_size": null,
  "outcomes": [
    "DNA strand breaks (alkaline comet assay tail moment)"
  ],
  "main_findings": "In MO54 cells, ELFMF exposure alone (5, 50, 400 mT) did not significantly change comet assay tail moment versus control up to 400 mT. X-rays (5 Gy) increased DNA strand breaks, and subsequent ELFMF exposure at 50 and 400 mT (at 4°C/on ice) significantly increased tail moment compared with X-rays alone; at 37°C, no significant difference was observed between X-rays alone and X-rays plus 400 mT.",
  "effect_direction": "mixed",
  "limitations": [
    "In vitro cell model (human glioma MO54 cells)",
    "Exposure duration not reported in the abstract",
    "Power frequency value not specified in the abstract",
    "Sample size and replication details not reported in the abstract",
    "Effect depended on temperature/exposure conditions (4°C/on ice vs 37°C)"
  ],
  "evidence_strength": "low",
  "confidence": 0.74,
  "peer_reviewed_likely": "yes",
  "keywords": [
    "extremely low-frequency magnetic field",
    "ELFMF",
    "power frequency",
    "magnetic flux density",
    "mT",
    "X-rays",
    "DNA strand breaks",
    "alkaline comet assay",
    "tail moment",
    "human glioma cells",
    "MO54",
    "temperature dependence"
  ],
  "suggested_hubs": [
    {
      "slug": "el f-emf",
      "weight": 0.9,
      "reason": "Study examines extremely low-frequency magnetic fields at power frequency (5–400 mT)."
    },
    {
      "slug": "in-vitro-studies",
      "weight": 0.75,
      "reason": "Cell culture experiment assessing DNA strand breaks via comet assay."
    }
  ]
}

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AI-extracted fields are generated from the abstract and metadata and may be incomplete or incorrect. This material is for informational purposes and is not medical advice.

Identifiers and source

DOI: 10.1269/jrr.41.293
PubMed: 11210830

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