An examination of the effect of an AC pulsed electric field on cell mortality in SWLA-2 hybridomas.

{
  "study_type": "in_vitro",
  "exposure": {
    "band": null,
    "source": "pulsed AC electric field",
    "frequency_mhz": null,
    "sar_wkg": null,
    "duration": "samples examined at 24 hours"
  },
  "population": "SWLA-2 murine hybridoma cells",
  "sample_size": null,
  "outcomes": [
    "cell lysis",
    "cell mortality"
  ],
  "main_findings": "AC electric field pulses estimated to produce at least 1 V across the cell membrane appeared more effective at producing cell lysis than fields producing lower membrane voltages. At membrane voltages above 1 V, higher frequencies (in the 10 kHz range) appeared more effective than lower frequencies in producing cell lysis.",
  "effect_direction": "harm",
  "limitations": [
    "Described as an initial experimental setup and results",
    "No quantitative results provided in the abstract",
    "Sample size not reported",
    "Exposure parameters (exact field strengths/voltages at source) not fully specified beyond membrane voltage estimate"
  ],
  "evidence_strength": "very_low",
  "confidence": 0.74,
  "peer_reviewed_likely": "yes",
  "keywords": [
    "AC electric field pulses",
    "pulsed electric field",
    "cell lysis",
    "cell mortality",
    "hybridoma",
    "10 kHz"
  ],
  "suggested_hubs": [
    {
      "slug": "in-vitro-studies",
      "weight": 0.9,
      "reason": "Cell culture experiment assessing effects of pulsed AC electric fields on cell lysis/mortality."
    }
  ]
}