C-phycocyanin extraction assisted by pulsed electric field from Artrosphira platensis.

Food research international (Ottawa, Ont.) · 2017 · Benefit · Low evidence

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BenefitLow evidencePaper 2850

Study snapshot

PublicationFood research international (Ottawa, Ont.) · 2017

Study typeother

Reported directionBenefit

Evidence ratingLow

Abstract

This paper assesses the application of pulsed electric fields (PEF) to the fresh biomass of Artrhospira platensis in order to enhance the extraction of C-phycocyanin into aqueous media. Electroporation of A. platensis depended on both electric field strength and treatment duration. The minimum electric field intensity for detecting C-phycocyanin in the extraction medium was 15kV/cm after the application of a treatment time 150μs (50 pulses of 3μs). However higher electric field strength were required when shorter treatment times were applied. Response surface methodology was used in order to investigate the influence of electric field strength (15-25kV/cm), treatment time (60-150μs), and temperature of application of PEF (10-40°C) on C-phycocyanin extraction yield (PEY). The increment of the temperature PEF treatment reduced the electric field strength and the treatment time required to obtain a given PEY and, consequently decreased the total specific energy delivered by the treatment. For example, the increment of temperature from 10°C to 40°C permitted to reduce the electric field strength required to extract 100mg/g d of C-phycocyanin from 25 to 18kV/cm, and the specific energy input from 106.7 to 67.5kJ/Kg. Results obtained in this investigation demonstrated PEF's potential for selectively extraction C-phycocyanin from fresh A. platensis biomass. The purity of the C-phycocyanin extract obtained from the electroporated cells was higher than that obtained using other techniques based on the cell complete destruction.

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AI evidence extraction

Structured fields below were generated from the paper’s abstract and metadata to make the evidence easier to inspect. Automated extraction may be incomplete or incorrect; verify important details against the original paper.

Study typeother

Effect directionbenefit

PopulationFresh biomass of Arthrospira (Artrhospira) platensis

Sample sizeNot supplied

Exposure60–150 μs (e.g., 50 pulses of 3 μs; minimum reported 150 μs)

Evidence strengthLowConfidence: 74% · Peer reviewed: yes

Main findings

Pulsed electric field (PEF) treatment enhanced extraction of C-phycocyanin from fresh Arthrospira platensis biomass, with electroporation depending on electric field strength and treatment duration. The minimum reported field intensity to detect C-phycocyanin in the extraction medium was 15 kV/cm after 150 μs (50 pulses of 3 μs). Increasing treatment temperature (10–40°C) reduced the field strength and treatment time needed for a given extraction yield and lowered specific energy input (e.g., for 100 mg/g d extraction, 25 to 18 kV/cm and 106.7 to 67.5 kJ/kg when temperature increased from 10°C to 40°C). Purity of extract from electroporated cells was higher than extracts obtained using techniques based on complete cell destruction.

Outcomes measured

C-phycocyanin extraction yield (PEY) into aqueous media
Detection of C-phycocyanin in extraction medium
Purity of C-phycocyanin extract
Electroporation dependence on field strength and treatment duration
Specific energy input (kJ/kg)

Limitations

Sample size not reported in abstract
No details provided on experimental replication or statistical uncertainty beyond mention of response surface methodology
Study is on algal biomass processing, not human/animal health outcomes

View raw extracted JSON

{
  "study_type": "other",
  "exposure": {
    "band": null,
    "source": "pulsed electric field (PEF) food/biomass processing",
    "frequency_mhz": null,
    "sar_wkg": null,
    "duration": "60–150 μs (e.g., 50 pulses of 3 μs; minimum reported 150 μs)"
  },
  "population": "Fresh biomass of Arthrospira (Artrhospira) platensis",
  "sample_size": null,
  "outcomes": [
    "C-phycocyanin extraction yield (PEY) into aqueous media",
    "Detection of C-phycocyanin in extraction medium",
    "Purity of C-phycocyanin extract",
    "Electroporation dependence on field strength and treatment duration",
    "Specific energy input (kJ/kg)"
  ],
  "main_findings": "Pulsed electric field (PEF) treatment enhanced extraction of C-phycocyanin from fresh Arthrospira platensis biomass, with electroporation depending on electric field strength and treatment duration. The minimum reported field intensity to detect C-phycocyanin in the extraction medium was 15 kV/cm after 150 μs (50 pulses of 3 μs). Increasing treatment temperature (10–40°C) reduced the field strength and treatment time needed for a given extraction yield and lowered specific energy input (e.g., for 100 mg/g d extraction, 25 to 18 kV/cm and 106.7 to 67.5 kJ/kg when temperature increased from 10°C to 40°C). Purity of extract from electroporated cells was higher than extracts obtained using techniques based on complete cell destruction.",
  "effect_direction": "benefit",
  "limitations": [
    "Sample size not reported in abstract",
    "No details provided on experimental replication or statistical uncertainty beyond mention of response surface methodology",
    "Study is on algal biomass processing, not human/animal health outcomes"
  ],
  "evidence_strength": "low",
  "confidence": 0.74,
  "peer_reviewed_likely": "yes",
  "keywords": [
    "pulsed electric field",
    "PEF",
    "electroporation",
    "Arthrospira platensis",
    "C-phycocyanin",
    "extraction yield",
    "temperature",
    "specific energy"
  ],
  "suggested_hubs": []
}

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AI-extracted fields are generated from the abstract and metadata and may be incomplete or incorrect. This material is for informational purposes and is not medical advice.

Identifiers and source

DOI: 10.1016/j.foodres.2016.09.029
PubMed: 28865615

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