An examination of the effect of decaying exponential pulse electric fields on cell mortality in murine spleenocytes, hybridomas, and human natural killer cells.

Conference proceedings : ... Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE Engineering in Medicine and Biology Society. Annual Conference · 2004 · Harm · Low evidence

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HarmLow evidencePaper 3625

Exponentially decaying electric field pulses produced substantial cell lysis/mortality across three cultured cell types. At 0.3 kV, murine spleenocytes showed ~50% mortality at 24 hours and ~25% viable cells at 48 hours. Other cell types showed mortality cons…

Study snapshot

PublicationConference proceedings : ... Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE Engineering in Medicine and Biology Society. Annual Conference · 2004

Study typein_vitro

Reported directionHarm

Evidence ratingLow

Abstract

This work describes the percentage cell lysis produced by exponentially decaying electric field pulses of varying amplitudes and time constants. Three different cell types were examined: murine spleenocytes, hybridomas, and human natural killer. Cells were cultured and separate samples examined at 24 hours and 48 hours. Two sets of experiments were performed for each cell type. At 0.3 kV, the spleenocytes exhibited a mortality of roughly 50% twenty-four hours after exposure to the pulse; while at forty-eight hours the spleenocyte cell count had reduced to roughly 25% viable cells. All other cell types showed mortality consistently in excess of 80% at field pulse strengths of about 0.3 V/m.

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AI evidence extraction

Structured fields below were generated from the paper’s abstract and metadata to make the evidence easier to inspect. Automated extraction may be incomplete or incorrect; verify important details against the original paper.

Study typein_vitro

Effect directionharm

PopulationMurine spleenocytes, hybridomas, and human natural killer cells (cultured)

Sample sizeNot supplied

Exposuresamples examined at 24 hours and 48 hours post-exposure; pulse time constants varied (not specified)

Evidence strengthLowConfidence: 66% · Peer reviewed: unknown

Main findings

Outcomes measured

cell lysis
cell mortality/viability at 24 hours
cell mortality/viability at 48 hours

Limitations

Sample size not reported in abstract
Exposure parameters incompletely specified (exact amplitudes/units inconsistent; time constants not provided)
No control/sham details reported
Only short follow-up timepoints (24 and 48 hours) reported

View raw extracted JSON

{
  "study_type": "in_vitro",
  "exposure": {
    "band": null,
    "source": "pulsed electric field exposure (exponentially decaying pulses)",
    "frequency_mhz": null,
    "sar_wkg": null,
    "duration": "samples examined at 24 hours and 48 hours post-exposure; pulse time constants varied (not specified)"
  },
  "population": "Murine spleenocytes, hybridomas, and human natural killer cells (cultured)",
  "sample_size": null,
  "outcomes": [
    "cell lysis",
    "cell mortality/viability at 24 hours",
    "cell mortality/viability at 48 hours"
  ],
  "main_findings": "Exponentially decaying electric field pulses produced substantial cell lysis/mortality across three cultured cell types. At 0.3 kV, murine spleenocytes showed ~50% mortality at 24 hours and ~25% viable cells at 48 hours. Other cell types showed mortality consistently >80% at field pulse strengths of about 0.3 V/m.",
  "effect_direction": "harm",
  "limitations": [
    "Sample size not reported in abstract",
    "Exposure parameters incompletely specified (exact amplitudes/units inconsistent; time constants not provided)",
    "No control/sham details reported",
    "Only short follow-up timepoints (24 and 48 hours) reported"
  ],
  "evidence_strength": "low",
  "confidence": 0.66,
  "peer_reviewed_likely": "unknown",
  "keywords": [
    "pulsed electric fields",
    "exponentially decaying pulses",
    "cell lysis",
    "cell mortality",
    "murine spleenocytes",
    "hybridomas",
    "human natural killer cells",
    "in vitro"
  ],
  "suggested_hubs": []
}

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AI-extracted fields are generated from the abstract and metadata and may be incomplete or incorrect. This material is for informational purposes and is not medical advice.

Identifiers and source

DOI: 10.1109/iembs.2004.1403758
PubMed: 17270818

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