Study of the effects of 50 Hz homogeneous magnetic field on apoptosis and proliferation of SNU cells in vitro.

Conference proceedings : ... Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE Engineering in Medicine and Biology Society. Annual Conference · 2004 · Mixed · Low evidence

← Research stream

MixedLow evidencePaper 3618

SNU cells exposed to a 50 Hz homogeneous ferromagnetic field (0.097 T) for 5–120 minutes had significantly higher apoptosis rates than controls, with a time-dependent increase from 5 to 60 minutes (r=0.97, P<0.01). Flow cytometry indicated no significant effe…

Study snapshot

PublicationConference proceedings : ... Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE Engineering in Medicine and Biology Society. Annual Conference · 2004

Study typein_vitro

Reported directionMixed

Evidence ratingLow

Abstract

The biological effects of magnetic field on SNU (human low differentiated gastric adenocarcinoma cell line) cells are studied in this paper. SNU cells were randomly divided into control groups and four treatment groups, the treatment groups were respectively irradiated by 50 Hz ferromagnetic field (0.097 T) 5 min., 30 min., 60 min., and 120 min. We observed the effects on proliferation and apoptosis of SNU cells after the treatment of magnetic field by flow cytometric (FCM) DNA analysis and electron microscope observation. The apoptosis rate in treatment groups are 4.75% +/- 1.24%, 6.50% +/- 0.61%, 9.20% +/- 1.76% and 6.26% +/- 2.95% respectively and significantly higher than that in control group. In the irradiation time range from 5 min to 60 min, there is a significant time-depended response correlation of apoptosis rate( r = 0.97, P < 0.01), but FCM results showed that the effects of 50 Hz homogeneous ferromagnetic field on the proliferation and distribution of cell cycle of SNU cells is not significant. We concluded that irradiation of 50 Hz homogeneous ferromagnetic field could promote apoptosis of SNU cells.

Machine Enhanced Logic

AI evidence extraction

Structured fields below were generated from the paper’s abstract and metadata to make the evidence easier to inspect. Automated extraction may be incomplete or incorrect; verify important details against the original paper.

Study typein_vitro

Effect directionmixed

PopulationSNU (human low differentiated gastric adenocarcinoma cell line) cells

Sample sizeNot supplied

ExposureELF · 0.05 MHz · 5, 30, 60, or 120 min

Evidence strengthLowConfidence: 86% · Peer reviewed: yes

Main findings

Outcomes measured

Apoptosis rate
Cell proliferation
Cell cycle distribution

Limitations

Sample size not reported in abstract
In vitro cell-line study; generalizability to humans not addressed
Exposure described as 0.097 T ferromagnetic field; dosimetry beyond field strength not provided
Only short-term exposures (minutes) assessed

View raw extracted JSON

{
  "study_type": "in_vitro",
  "exposure": {
    "band": "ELF",
    "source": null,
    "frequency_mhz": 0.05,
    "sar_wkg": null,
    "duration": "5, 30, 60, or 120 min"
  },
  "population": "SNU (human low differentiated gastric adenocarcinoma cell line) cells",
  "sample_size": null,
  "outcomes": [
    "Apoptosis rate",
    "Cell proliferation",
    "Cell cycle distribution"
  ],
  "main_findings": "SNU cells exposed to a 50 Hz homogeneous ferromagnetic field (0.097 T) for 5–120 minutes had significantly higher apoptosis rates than controls, with a time-dependent increase from 5 to 60 minutes (r=0.97, P<0.01). Flow cytometry indicated no significant effects on proliferation or cell-cycle distribution.",
  "effect_direction": "mixed",
  "limitations": [
    "Sample size not reported in abstract",
    "In vitro cell-line study; generalizability to humans not addressed",
    "Exposure described as 0.097 T ferromagnetic field; dosimetry beyond field strength not provided",
    "Only short-term exposures (minutes) assessed"
  ],
  "evidence_strength": "low",
  "confidence": 0.86,
  "peer_reviewed_likely": "yes",
  "keywords": [
    "50 Hz",
    "ELF magnetic field",
    "homogeneous magnetic field",
    "ferromagnetic field",
    "0.097 T",
    "SNU cells",
    "gastric adenocarcinoma cell line",
    "apoptosis",
    "proliferation",
    "cell cycle",
    "flow cytometry",
    "electron microscopy"
  ],
  "suggested_hubs": [
    {
      "slug": "elf-emf",
      "weight": 0.9,
      "reason": "Study examines biological effects of 50 Hz magnetic field exposure."
    }
  ]
}

AI can be wrong. Always verify against the paper.

AI-extracted fields are generated from the abstract and metadata and may be incomplete or incorrect. This material is for informational purposes and is not medical advice.

Identifiers and source

DOI: 10.1109/iembs.2004.1404379
PubMed: 17271435

Read the original paper →

Related papers

← Back to the Research Library