Study of the Effects of 50Hz Homogeneous Magnetic Field on Expression of Bax, Bcl-2 and Caspase-3 of SNU Cells In Vitro.

Conference proceedings : ... Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE Engineering in Medicine and Biology Society. Annual Conference · 2005 · Harm · Low evidence

← Research stream

HarmLow evidencePaper 3548

Compared with control, all 50 Hz magnetic field exposure groups (0.097 T; 5–120 min) showed higher Bax and Caspase-3 expression and lower Bcl-2 expression by flow cytometry, immunocytochemical staining, and Western blot.

Study snapshot

PublicationConference proceedings : ... Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE Engineering in Medicine and Biology Society. Annual Conference · 2005

Study typein_vitro

Reported directionHarm

Evidence ratingLow

Abstract

We investigate the effects of magnetic field on expression of Bax, Bcl-2 and Caspase-3 of SNU (human low differentiated gastric adenocarcinoma cell line) in this article. SNU cells were randomly divided into control groups and four treatment groups, the treatment groups were respectively irradiated by 50Hz homogeneous ferromagnetic field (0.097T) 5min, 30min, 60min and 120min. SNU cells were harvested for detection of Bax, Bcl-2 and Caspase-3 antigen with flow cytometric analysis (FCM), immunocytochemical staining and Western blot. The expression of Bax and Caspase-3 in all magnetic field exposure groups were higher than that in control by FCM, immunocytochemical stain and Western blot, but the expression of Bcl-2 in all magnetic field exposure groups were lower than that in control by FCM, immunocytochemical stain and Western blot. We concluded that possible mechanisms of apoptosis of SNU cells after 50Hz homogeneous ferromagnetic field (0.097T) treatment may be Up-regulating the expression of Bax and Caspase-3, dowm-regulating the expression of Bcl-2.

Machine Enhanced Logic

AI evidence extraction

Structured fields below were generated from the paper’s abstract and metadata to make the evidence easier to inspect. Automated extraction may be incomplete or incorrect; verify important details against the original paper.

Study typein_vitro

Effect directionharm

PopulationSNU (human low differentiated gastric adenocarcinoma) cell line

Sample sizeNot supplied

ExposureELF · 5, 30, 60, or 120 min

Evidence strengthLowConfidence: 78% · Peer reviewed: yes

Main findings

Outcomes measured

Bax expression
Bcl-2 expression
Caspase-3 expression

Limitations

In vitro cell-line study; findings may not generalize to humans or in vivo conditions
Sample size not reported in abstract
Exposure metric reported as magnetic flux density (T) without additional dosimetry details

View raw extracted JSON

{
  "study_type": "in_vitro",
  "exposure": {
    "band": "ELF",
    "source": null,
    "frequency_mhz": null,
    "sar_wkg": null,
    "duration": "5, 30, 60, or 120 min"
  },
  "population": "SNU (human low differentiated gastric adenocarcinoma) cell line",
  "sample_size": null,
  "outcomes": [
    "Bax expression",
    "Bcl-2 expression",
    "Caspase-3 expression"
  ],
  "main_findings": "Compared with control, all 50 Hz magnetic field exposure groups (0.097 T; 5–120 min) showed higher Bax and Caspase-3 expression and lower Bcl-2 expression by flow cytometry, immunocytochemical staining, and Western blot.",
  "effect_direction": "harm",
  "limitations": [
    "In vitro cell-line study; findings may not generalize to humans or in vivo conditions",
    "Sample size not reported in abstract",
    "Exposure metric reported as magnetic flux density (T) without additional dosimetry details"
  ],
  "evidence_strength": "low",
  "confidence": 0.78,
  "peer_reviewed_likely": "yes",
  "keywords": [
    "50 Hz",
    "ELF magnetic field",
    "0.097 T",
    "SNU cells",
    "gastric adenocarcinoma cell line",
    "apoptosis",
    "Bax",
    "Bcl-2",
    "Caspase-3",
    "flow cytometry",
    "immunocytochemical staining",
    "Western blot"
  ],
  "suggested_hubs": [
    {
      "slug": "elf-emf",
      "weight": 0.9,
      "reason": "50 Hz magnetic field exposure (ELF) in vitro."
    }
  ]
}

AI can be wrong. Always verify against the paper.

AI-extracted fields are generated from the abstract and metadata and may be incomplete or incorrect. This material is for informational purposes and is not medical advice.

Identifiers and source

DOI: 10.1109/iembs.2005.1615563
PubMed: 17281333

Read the original paper →

Related papers

← Back to the Research Library