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[Effect of microwave radiation on primary cultured Sertoli cells].

Zhonghua lao dong wei sheng zhi ye bing za zhi = Zhonghua laodong weisheng zhiyebing zazhi = Chinese journal of industrial hygiene and occupational diseases · 2009 · Mixed · Low evidence

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MixedLow evidencePaper 3365

After 30 mW/cm^2 microwave exposure for 5 minutes, cell cycle distribution differed from controls (reduced G0-G1 and G2-M; increased S phase), while apoptosis/death and intracellular Ca2+ showed no difference at 6 hours. After 100 mW/cm^2 exposure, cells show…

Study snapshot

PublicationZhonghua lao dong wei sheng zhi ye bing za zhi = Zhonghua laodong weisheng zhiyebing zazhi = Chinese journal of industrial hygiene and occupational diseases · 2009
Study typein_vitro
Reported directionMixed
Evidence ratingLow

Abstract

OBJECTIVE: To explore whether microwave radiation may cause injury of primary cultured Sertoli cells. METHODS: The model of primary cultured Sertoli cells in vitro was established, which was radiated by microwave with average power density 0, 30 and 100 mW/cm(2) for five minutes. The changes of cell cycle, apoptosis and death, and intracellular Ca2+ concentration in the Sertoli cells were measured at sixth hours through Annexin V-PI double labeling and Fluo-3-AM labeling, flow cytometry combined with laser scanning confocal microscopy after microwave exposure. RESULTS: The numbers of Sertoli cells were obviously reduced in G0-G1 and G2-M phase (62.57% +/- 3.22% and 8.25% +/- 1.75%) and increased in S phase (29.17% +/- 4.87%) compared with the control groups (79.18% +/- 0.24%, 11.17% +/- 0.50% and 9.64% +/- 0.62%) (P < 0.05 or P < 0.01), but the changes of rate of apoptosis and death and intracellular Ca2+ concentration showed no difference at 6 h after exposure to 30 mW/cm(2) microwave. There was a significant increase in the Sertoli cell counts of G0-G1 phase (87.69% +/- 1.32%), and decrease in the Sertoli cell counts of G2-M and S phase (7.41% +/- 0.60% and 4.87% +/- 0.91%) (P < 0.01). There was also a significant increase in intracellular Ca2+ concentration and rate of apoptosis and death (P < 0.05 or P < 0.01) at 6 h after exposure to 100 mW/cm(2) microwave. CONCLUSION: 100 mW/cm(2) microwave radiation may cause growth inhibition and increase of apoptosis and death in the primary cultured Sertoli cells. The increase of intracellular Ca2+ concentration is one of the injury mechanisms.

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Structured fields below were generated from the paper’s abstract and metadata to make the evidence easier to inspect. Automated extraction may be incomplete or incorrect; verify important details against the original paper.

Study typein_vitro
Effect directionmixed
PopulationPrimary cultured Sertoli cells (in vitro)
Sample sizeNot supplied
Exposuremicrowave · 5 minutes
Evidence strengthLowConfidence: 78% · Peer reviewed: yes

Main findings

After 30 mW/cm^2 microwave exposure for 5 minutes, cell cycle distribution differed from controls (reduced G0-G1 and G2-M; increased S phase), while apoptosis/death and intracellular Ca2+ showed no difference at 6 hours. After 100 mW/cm^2 exposure, cells showed increased G0-G1 with decreased S and G2-M, along with increased intracellular Ca2+ and increased apoptosis/death at 6 hours.

Outcomes measured

  • Cell cycle distribution (G0-G1, S, G2-M)
  • Apoptosis and cell death (Annexin V-PI)
  • Intracellular Ca2+ concentration (Fluo-3-AM)

Limitations

  • In vitro study in primary cultured Sertoli cells; generalizability to in vivo/humans not addressed
  • Microwave frequency not reported in abstract
  • Sample size not reported in abstract
  • Only one post-exposure time point reported (6 hours)
  • Exposure duration limited to 5 minutes
View raw extracted JSON
{
  "study_type": "in_vitro",
  "exposure": {
    "band": "microwave",
    "source": null,
    "frequency_mhz": null,
    "sar_wkg": null,
    "duration": "5 minutes"
  },
  "population": "Primary cultured Sertoli cells (in vitro)",
  "sample_size": null,
  "outcomes": [
    "Cell cycle distribution (G0-G1, S, G2-M)",
    "Apoptosis and cell death (Annexin V-PI)",
    "Intracellular Ca2+ concentration (Fluo-3-AM)"
  ],
  "main_findings": "After 30 mW/cm^2 microwave exposure for 5 minutes, cell cycle distribution differed from controls (reduced G0-G1 and G2-M; increased S phase), while apoptosis/death and intracellular Ca2+ showed no difference at 6 hours. After 100 mW/cm^2 exposure, cells showed increased G0-G1 with decreased S and G2-M, along with increased intracellular Ca2+ and increased apoptosis/death at 6 hours.",
  "effect_direction": "mixed",
  "limitations": [
    "In vitro study in primary cultured Sertoli cells; generalizability to in vivo/humans not addressed",
    "Microwave frequency not reported in abstract",
    "Sample size not reported in abstract",
    "Only one post-exposure time point reported (6 hours)",
    "Exposure duration limited to 5 minutes"
  ],
  "evidence_strength": "low",
  "confidence": 0.78,
  "peer_reviewed_likely": "yes",
  "keywords": [
    "microwave radiation",
    "Sertoli cells",
    "cell cycle",
    "apoptosis",
    "cell death",
    "intracellular calcium",
    "flow cytometry",
    "confocal microscopy",
    "power density"
  ],
  "suggested_hubs": []
}
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AI-extracted fields are generated from the abstract and metadata and may be incomplete or incorrect. This material is for informational purposes and is not medical advice.

Identifiers and source

DOI: Not supplied
PubMed: 20137297

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