25 Hz electromagnetic field exposure has no effect on cell cycle distribution and apoptosis in U-937 and HCA-2/1cch cells.

Bioelectrochemistry (Amsterdam, Netherlands) · 2001 · Mixed · Low evidence

← Research stream

MixedLow evidencePaper 5324

U-937 and HCA-2/1cch cells exposed to a 25 Hz, 1.5 mT ELF-EMF for 2 h 45 min showed no significant alterations in cell cycle distribution and no induction of apoptosis. In HCA-2/1cch cells exposed in the presence of dexamethasone, relative cell number decreas…

Study snapshot

PublicationBioelectrochemistry (Amsterdam, Netherlands) · 2001

Study typein_vitro

Reported directionMixed

Evidence ratingLow

Abstract

It is reported that exposure to 50 Hz extremely low-frequency electromagnetic field (ELF-EMF) can produce apoptosis and small variations in cell cycle distribution on different cell lines. In order to study the effect of ELF-EMF on tumoral cells in vitro, two cell lines (U-937, from a histiocytic lymphoma, and HCA-2/1cch, from a human colon adenocarcinoma) were exposed to 25 Hz, 1.5 mT, for 2 h and 45 min. Cell cycle distribution, apoptosis (spontaneous and dexamethasone-induced) and cell growth were evaluated. Neither significant alteration in cell cycle phases nor induction of apoptosis was observed. Nevertheless, the relative cell number was found to decrease to 55.84+/-7.35% (p <0.05, Student's t-test) for HCA-2/1cch cells after exposure to EMF in the presence of dexamethasone. The presence of dexamethasone during the EMF exposure could probably produce a decrease in the cell growth of this cell line.

Machine Enhanced Logic

AI evidence extraction

Structured fields below were generated from the paper’s abstract and metadata to make the evidence easier to inspect. Automated extraction may be incomplete or incorrect; verify important details against the original paper.

Study typein_vitro

Effect directionmixed

PopulationU-937 (histiocytic lymphoma) and HCA-2/1cch (human colon adenocarcinoma) cell lines

Sample sizeNot supplied

ExposureELF · 0.025 MHz · 2 h 45 min

Evidence strengthLowConfidence: 78% · Peer reviewed: yes

Main findings

Outcomes measured

Cell cycle distribution
Apoptosis (spontaneous and dexamethasone-induced)
Cell growth / relative cell number

Limitations

In vitro study (cell lines)
Sample size not reported in abstract
Exposure conditions limited to 25 Hz, 1.5 mT, 2 h 45 min
Observed decrease in relative cell number reported only for one cell line and in the presence of dexamethasone

View raw extracted JSON

{
  "study_type": "in_vitro",
  "exposure": {
    "band": "ELF",
    "source": null,
    "frequency_mhz": 0.025,
    "sar_wkg": null,
    "duration": "2 h 45 min"
  },
  "population": "U-937 (histiocytic lymphoma) and HCA-2/1cch (human colon adenocarcinoma) cell lines",
  "sample_size": null,
  "outcomes": [
    "Cell cycle distribution",
    "Apoptosis (spontaneous and dexamethasone-induced)",
    "Cell growth / relative cell number"
  ],
  "main_findings": "U-937 and HCA-2/1cch cells exposed to a 25 Hz, 1.5 mT ELF-EMF for 2 h 45 min showed no significant alterations in cell cycle distribution and no induction of apoptosis. In HCA-2/1cch cells exposed in the presence of dexamethasone, relative cell number decreased to 55.84±7.35% (p<0.05).",
  "effect_direction": "mixed",
  "limitations": [
    "In vitro study (cell lines)",
    "Sample size not reported in abstract",
    "Exposure conditions limited to 25 Hz, 1.5 mT, 2 h 45 min",
    "Observed decrease in relative cell number reported only for one cell line and in the presence of dexamethasone"
  ],
  "evidence_strength": "low",
  "confidence": 0.78,
  "peer_reviewed_likely": "yes",
  "keywords": [
    "extremely low-frequency",
    "ELF-EMF",
    "25 Hz",
    "1.5 mT",
    "U-937",
    "HCA-2/1cch",
    "cell cycle",
    "apoptosis",
    "dexamethasone",
    "cell growth"
  ],
  "suggested_hubs": []
}

AI can be wrong. Always verify against the paper.

AI-extracted fields are generated from the abstract and metadata and may be incomplete or incorrect. This material is for informational purposes and is not medical advice.

Identifiers and source

DOI: 10.1016/s0302-4598(00)00119-7
PubMed: 11206921

Read the original paper →

Related papers

← Back to the Research Library