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Effect of rosmarinic acid on sertoli cells apoptosis and serum antioxidant levels in rats after exposure to electromagnetic fields.

African journal of traditional, complementary, and alternative medicines : AJTCAM · 2013 · Mixed · Low evidence

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MixedLow evidencePaper 4556

Rats exposed to 50 Hz EMF and given rosmarinic acid (5 mg/rat by gavage) showed significantly decreased MDA levels and Sertoli cell apoptosis compared with experimental groups (P<0.05). Testosterone and total antioxidant capacity (TAC) significantly increased…

Study snapshot

PublicationAfrican journal of traditional, complementary, and alternative medicines : AJTCAM · 2013
Study typeanimal
Reported directionMixed
Evidence ratingLow

Abstract

Rosmarinic acid belongs to the group of polyphenols; it has antioxidant, anti-inflammatory and antimicrobial activities and help to prevent cell damage caused by free radicals. The objective was to study the effect of Rosmarinic acid on sertolli cells apoptosis and serum antioxidant levels in rats after they were exposed to electromagnetic fields. Male Wistar rats (n=40) were allocated into three groups: control group (n=10) that received 5 cc normal saline (0.9% NaCl) daily by gavage method, Rosmarinic acid group that received 5mg/rat (gavage) (n=10), electromagnetic fields (EMF) group that had exposure with 50 hz (n=20) which was subdivided to two groups of 10; EMF group and treatment group. Treatment group received 5mg/rat (gavage) Rosmarinic acid daily for 6 weeks, respectively. However, the control group just received an equal volume of distilled water daily (gavage). On the 42nd day of research, 5 cc blood was collected to measure testosterone hormones, total antioxidant capacity (TAC), levels from whole group's analysis. Level of malondialdehyde (MDA) levels and sertoli cells apoptosis significantly decreased in the group that received 5mg/rat of Rosmarinic acid (P<0.05) in comparison with experimental groups. Level of testosterone, total antioxidant capacity (TAC), significantly increased in groups that received Rosmarinic acid (P<0.05). Since in our study 5mg/rat of Rosmarinic acid showed significantly preventive effect on cell damages especial sertoli cells apoptosis that caused with EMF, it seems that using Rosmarinic acid as food additive can be effective for supporting people living under EMF environmental pollution.

Machine Enhanced Logic

AI evidence extraction

Structured fields below were generated from the paper’s abstract and metadata to make the evidence easier to inspect. Automated extraction may be incomplete or incorrect; verify important details against the original paper.

Study typeanimal
Effect directionmixed
PopulationMale Wistar rats
Sample size40
ExposureELF · 0.05 MHz · 6 weeks
Evidence strengthLowConfidence: 74% · Peer reviewed: yes

Main findings

Rats exposed to 50 Hz EMF and given rosmarinic acid (5 mg/rat by gavage) showed significantly decreased MDA levels and Sertoli cell apoptosis compared with experimental groups (P<0.05). Testosterone and total antioxidant capacity (TAC) significantly increased in groups receiving rosmarinic acid (P<0.05).

Outcomes measured

  • Sertoli cell apoptosis
  • Serum antioxidant levels / total antioxidant capacity (TAC)
  • Malondialdehyde (MDA)
  • Testosterone

Limitations

  • EMF exposure intensity and exposure setup not described in the abstract
  • Comparisons are described generally (e.g., "experimental groups") without specifying all pairwise contrasts
  • Only male rats were studied; generalizability to humans is not addressed in the abstract
View raw extracted JSON
{
  "study_type": "animal",
  "exposure": {
    "band": "ELF",
    "source": null,
    "frequency_mhz": 0.05,
    "sar_wkg": null,
    "duration": "6 weeks"
  },
  "population": "Male Wistar rats",
  "sample_size": 40,
  "outcomes": [
    "Sertoli cell apoptosis",
    "Serum antioxidant levels / total antioxidant capacity (TAC)",
    "Malondialdehyde (MDA)",
    "Testosterone"
  ],
  "main_findings": "Rats exposed to 50 Hz EMF and given rosmarinic acid (5 mg/rat by gavage) showed significantly decreased MDA levels and Sertoli cell apoptosis compared with experimental groups (P<0.05). Testosterone and total antioxidant capacity (TAC) significantly increased in groups receiving rosmarinic acid (P<0.05).",
  "effect_direction": "mixed",
  "limitations": [
    "EMF exposure intensity and exposure setup not described in the abstract",
    "Comparisons are described generally (e.g., \"experimental groups\") without specifying all pairwise contrasts",
    "Only male rats were studied; generalizability to humans is not addressed in the abstract"
  ],
  "evidence_strength": "low",
  "confidence": 0.74,
  "peer_reviewed_likely": "yes",
  "keywords": [
    "rosmarinic acid",
    "electromagnetic fields",
    "50 Hz",
    "ELF",
    "Sertoli cells",
    "apoptosis",
    "malondialdehyde",
    "total antioxidant capacity",
    "testosterone",
    "rat"
  ],
  "suggested_hubs": [
    {
      "slug": "occupational-exposure",
      "weight": 0.2,
      "reason": "Study involves ELF (50 Hz) EMF exposure, which can relate to power-frequency/occupational contexts, though the source is not specified."
    }
  ]
}
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AI-extracted fields are generated from the abstract and metadata and may be incomplete or incorrect. This material is for informational purposes and is not medical advice.

Identifiers and source

DOI: 10.4314/ajtcam.v10i6.14
PubMed: 24311872

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