Radiofrequency electromagnetic fields do not alter the cell cycle progression of C3H 10T and U87MG cells.

Radiation research · 2001 · No effect · Low evidence

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No effectLow evidencePaper 5314

Study snapshot

PublicationRadiation research · 2001

Study typein_vitro

Reported directionNo effect

Evidence ratingLow

Abstract

The effects of exposure to radiofrequency electromagnetic fields (RF EMFs) on cell cycle progression of mouse fibroblasts C3H 10T(1/2) and human glioma U87MG cells were determined by the flow cytometric bromodeoxyuridine pulse-chase method. Cells were exposed to a frequency-modulated continuous wave at 835.62 MHz or a code division multiple access RF EMF centered on 847.74 MHz at an average specific absorption rate of 0.6 W/kg. Five cell cycle parameters, including the transit of cells through G(1), G(2) and S phase and the probability of cell division, were examined immediately after the cells were placed in the fields or after they had been kept in the fields for up to 100 h. The only significant change observed in the study was that associated with C3H 10T(1/2) cell cultures moving into plateau phase toward the later times in the long-exposure experiment. No changes in the cell cycle parameters were observed in cells exposed to either mode of RF EMFs when compared to sham-exposed cells in either of the cell lines studied during the entire experimental period. The results show that exposure to RF EMFs, at the frequencies and power tested, does not have any effect on cell progression in vitro.

Machine Enhanced Logic

AI evidence extraction

Structured fields below were generated from the paper’s abstract and metadata to make the evidence easier to inspect. Automated extraction may be incomplete or incorrect; verify important details against the original paper.

Study typein_vitro

Effect directionno_effect

PopulationMouse fibroblasts C3H 10T(1/2) and human glioma U87MG cells (in vitro)

Sample sizeNot supplied

ExposureRF · 0.6 W/kg · immediately after exposure start or up to 100 h

Evidence strengthLowConfidence: 86% · Peer reviewed: yes

Main findings

Cells exposed to frequency-modulated continuous wave at 835.62 MHz or CDMA RF EMF centered on 847.74 MHz at an average SAR of 0.6 W/kg showed no changes in five cell-cycle parameters compared with sham-exposed cells across the experimental period (immediate to 100 h). The only significant change noted was attributed to C3H 10T(1/2) cultures moving into plateau phase at later times in the long-exposure experiment.

Outcomes measured

Cell cycle progression (G1, S, G2 transit)
Probability of cell division

Limitations

Sample size not reported in abstract
In vitro study (cell lines), which may not generalize to in vivo/human health outcomes
Only two RF signal types/frequencies and one average SAR (0.6 W/kg) tested
Outcomes limited to cell-cycle parameters over up to 100 h

View raw extracted JSON

{
  "study_type": "in_vitro",
  "exposure": {
    "band": "RF",
    "source": null,
    "frequency_mhz": null,
    "sar_wkg": 0.6,
    "duration": "immediately after exposure start or up to 100 h"
  },
  "population": "Mouse fibroblasts C3H 10T(1/2) and human glioma U87MG cells (in vitro)",
  "sample_size": null,
  "outcomes": [
    "Cell cycle progression (G1, S, G2 transit)",
    "Probability of cell division"
  ],
  "main_findings": "Cells exposed to frequency-modulated continuous wave at 835.62 MHz or CDMA RF EMF centered on 847.74 MHz at an average SAR of 0.6 W/kg showed no changes in five cell-cycle parameters compared with sham-exposed cells across the experimental period (immediate to 100 h). The only significant change noted was attributed to C3H 10T(1/2) cultures moving into plateau phase at later times in the long-exposure experiment.",
  "effect_direction": "no_effect",
  "limitations": [
    "Sample size not reported in abstract",
    "In vitro study (cell lines), which may not generalize to in vivo/human health outcomes",
    "Only two RF signal types/frequencies and one average SAR (0.6 W/kg) tested",
    "Outcomes limited to cell-cycle parameters over up to 100 h"
  ],
  "evidence_strength": "low",
  "confidence": 0.86,
  "peer_reviewed_likely": "yes",
  "keywords": [
    "radiofrequency electromagnetic fields",
    "RF EMF",
    "in vitro",
    "cell cycle",
    "flow cytometry",
    "bromodeoxyuridine pulse-chase",
    "C3H 10T(1/2)",
    "U87MG",
    "CDMA",
    "835.62 MHz",
    "847.74 MHz",
    "SAR 0.6 W/kg"
  ],
  "suggested_hubs": []
}

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AI-extracted fields are generated from the abstract and metadata and may be incomplete or incorrect. This material is for informational purposes and is not medical advice.

Identifiers and source

DOI: 10.1667/0033-7587(2001)156[0786:refdna]2.0.co;2
PubMed: 11741503

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