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Effects of 50 Hz MF exposure on DNA damage and cellular functions in various neurogenic cells

2017 · No effect · Low evidence

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No effectLow evidencePaper 2306

Exposure to a 50 Hz magnetic field at 2.0 mT for up to 24 h did not influence γH2AX foci formation in six neurogenic cell types and did not affect cell cycle progression, proliferation, or viability in the tumor cell lines. The exposure did not significantly …

Study snapshot

Publicationmanual · 2017
Study typein_vitro
Reported directionNo effect
Evidence ratingLow

Abstract

Effects of 50 Hz MF exposure on DNA damage and cellular functions in various neurogenic cells Su L, Yimaer A, Wei X, Xu Z, Chen G. The effects of 50 Hz magnetic field exposure on DNA damage and cellular functions in various neurogenic cells.J Radiat Res. 2017 Mar 21:1-13. doi: 10.1093/jrr/rrx012. Abstract Epidemiological studies have indicated a possible association between extremely low-frequency magnetic field (ELF-MF) exposure and the risk of nervous system diseases. However, laboratory studies have not provided consistent results for clarifying this association, despite many years of studies. In this study, we have systematically investigated the effects of 50 Hz MF exposure on DNA damage and cellular functions in both neurogenic tumor cell lines (U251, A172, SH-SY5Y) and primary cultured neurogenic cells from rats (astrocytes, microglia, cortical neurons). The results showed that exposure to a 50 Hz MF at 2.0 mT for up to 24 h did not influence γH2AX foci formation (an early marker of DNA double-strand breaks) in any of six different neurogenic cells. Exposure to a 50 Hz MF did not affect cell cycle progression, cell proliferation or cell viability in neurogenic tumor U251, A172 or SH-SY5Y cells. Furthermore, the MF exposure for 24 h did not significantly affect the secretion of cytokines (TNF-α, IL-6 or IL-1β) in astrocytes or microglia, or the phagocytic activity of microglia. In addition, MF exposure for 1 h per day did not significantly influence expression levels of microtubule-associated protein tau, microtubule-associated protein 2, postsynaptic density 95 or gephyrin in cortical neurons, indicating an absence of effects of MF exposure on the development of cortical neurons. In conclusion, our data suggest that exposure to a 50 Hz MF at 2.0 mT did not elicit DNA damage effects or abnormal cellular functions in the neurogenic cells studied. ncbi.nlm.nih.gov

Machine Enhanced Logic

AI evidence extraction

Structured fields below were generated from the paper’s abstract and metadata to make the evidence easier to inspect. Automated extraction may be incomplete or incorrect; verify important details against the original paper.

Study typein_vitro
Effect directionno_effect
PopulationNeurogenic tumor cell lines (U251, A172, SH-SY5Y) and primary cultured rat neurogenic cells (astrocytes, microglia, cortical neurons)
Sample sizeNot supplied
ExposureELF · 0.05 MHz · up to 24 h; also 1 h/day (duration not further specified)
Evidence strengthLowConfidence: 78% · Peer reviewed: yes

Main findings

Exposure to a 50 Hz magnetic field at 2.0 mT for up to 24 h did not influence γH2AX foci formation in six neurogenic cell types and did not affect cell cycle progression, proliferation, or viability in the tumor cell lines. The exposure did not significantly affect cytokine secretion in astrocytes or microglia, microglial phagocytosis, or (with 1 h/day exposure) expression of several neuronal development-related proteins in cortical neurons.

Outcomes measured

  • γH2AX foci formation (DNA double-strand break marker)
  • cell cycle progression
  • cell proliferation
  • cell viability
  • cytokine secretion (TNF-α, IL-6, IL-1β)
  • microglial phagocytic activity
  • neuronal protein expression (tau, MAP2, PSD95, gephyrin) / cortical neuron development

Limitations

  • Sample size not reported in abstract
  • Only one field strength reported (2.0 mT)
  • Exposure durations/regimens beyond those stated are not described in abstract
View raw extracted JSON
{
  "study_type": "in_vitro",
  "exposure": {
    "band": "ELF",
    "source": null,
    "frequency_mhz": 0.05,
    "sar_wkg": null,
    "duration": "up to 24 h; also 1 h/day (duration not further specified)"
  },
  "population": "Neurogenic tumor cell lines (U251, A172, SH-SY5Y) and primary cultured rat neurogenic cells (astrocytes, microglia, cortical neurons)",
  "sample_size": null,
  "outcomes": [
    "γH2AX foci formation (DNA double-strand break marker)",
    "cell cycle progression",
    "cell proliferation",
    "cell viability",
    "cytokine secretion (TNF-α, IL-6, IL-1β)",
    "microglial phagocytic activity",
    "neuronal protein expression (tau, MAP2, PSD95, gephyrin) / cortical neuron development"
  ],
  "main_findings": "Exposure to a 50 Hz magnetic field at 2.0 mT for up to 24 h did not influence γH2AX foci formation in six neurogenic cell types and did not affect cell cycle progression, proliferation, or viability in the tumor cell lines. The exposure did not significantly affect cytokine secretion in astrocytes or microglia, microglial phagocytosis, or (with 1 h/day exposure) expression of several neuronal development-related proteins in cortical neurons.",
  "effect_direction": "no_effect",
  "limitations": [
    "Sample size not reported in abstract",
    "Only one field strength reported (2.0 mT)",
    "Exposure durations/regimens beyond those stated are not described in abstract"
  ],
  "evidence_strength": "low",
  "confidence": 0.78,
  "peer_reviewed_likely": "yes",
  "keywords": [
    "50 Hz",
    "extremely low-frequency magnetic field",
    "ELF-MF",
    "2.0 mT",
    "DNA damage",
    "γH2AX",
    "neurogenic cells",
    "U251",
    "A172",
    "SH-SY5Y",
    "astrocytes",
    "microglia",
    "cortical neurons",
    "cytokines",
    "phagocytosis",
    "tau",
    "MAP2",
    "PSD95",
    "gephyrin"
  ],
  "suggested_hubs": [
    {
      "slug": "occupational-exposure",
      "weight": 0.25,
      "reason": "Study examines ELF magnetic field exposure (50 Hz), relevant to power-frequency exposure contexts."
    }
  ]
}
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AI-extracted fields are generated from the abstract and metadata and may be incomplete or incorrect. This material is for informational purposes and is not medical advice.

Identifiers and source

DOI: 10.1093/jrr/rrx012
PubMed: Not supplied

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