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Exposure to 50 Hz Extremely-Low-Frequency Magnetic Fields Induces No DNA Damage in Cells by Gamma H2AX Technology

2021 · No effect · Low evidence

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No effectLow evidencePaper 1232

Cells exposed to 50 Hz ELF-MF at 0.4, 1, and 2 mT for 15 min, 1 h, or 24 h showed no significant differences versus sham in γH2AX foci per cell, percentage of γH2AX foci-positive cells, or γH2AX expression (P > 0.05). The authors conclude no DNA damage was in…

Study snapshot

Publicationmanual · 2021
Study typein_vitro
Reported directionNo effect
Evidence ratingLow

Abstract

Exposure to 50 Hz Extremely-Low-Frequency Magnetic Fields Induces No DNA Damage in Cells by Gamma H2AX Technology Ye Lv, Shuchang Chen, Bing Zhu, Hong Xu, Shanshan Xu, Weiyan Liu, Yunyun Shen, Qunli Zeng. Exposure to 50 Hz Extremely-Low-Frequency Magnetic Fields Induces No DNA Damage in Cells by Gamma H2AX Technology. Biomed Res Int. 2021 Feb 15;2021:8510315. doi: 10.1155/2021/8510315. Abstract The current results for extremely-low-frequency magnetic fields (ELF-MF) on DNA damage are still debated. A sensitive indicator and systematic research are needed to assess the effects of ELF-MF. In this study, we used γH2AX as an early and sensitive molecular marker to evaluate the DNA damage effects of ELF-MF in vitro. Human amnion epithelial cells (FLs), human skin fibroblast cells (HSFs), and human umbilical vein endothelial cells (HUVECs) were exposed to 50 Hz ELF-MF at 0.4, 1, and 2 mT for 15 min, 1 h, and 24 h, respectively. After exposure, cells were subjected to γH2AX immunofluorescence and western blot. The results showed no significant difference in the average number of foci per cell, the percentage of γH2AX foci-positive cells, or the expression of γH2AX between the sham and 50 Hz ELF-MF exposure groups (P > 0.05). In conclusion, 50 Hz ELF- MF did not induce DNA damage in FLs, HSFs, or HUVECs, which was independent of the intensity or duration of the exposure. Open access paper: ncbi.nlm.nih.gov

Machine Enhanced Logic

AI evidence extraction

Structured fields below were generated from the paper’s abstract and metadata to make the evidence easier to inspect. Automated extraction may be incomplete or incorrect; verify important details against the original paper.

Study typein_vitro
Effect directionno_effect
PopulationHuman cell lines: human amnion epithelial cells (FLs), human skin fibroblast cells (HSFs), and human umbilical vein endothelial cells (HUVECs)
Sample sizeNot supplied
ExposureELF · 5e-05 MHz · 15 min, 1 h, and 24 h
Evidence strengthLowConfidence: 78% · Peer reviewed: yes

Main findings

Cells exposed to 50 Hz ELF-MF at 0.4, 1, and 2 mT for 15 min, 1 h, or 24 h showed no significant differences versus sham in γH2AX foci per cell, percentage of γH2AX foci-positive cells, or γH2AX expression (P > 0.05). The authors conclude no DNA damage was induced, independent of exposure intensity or duration.

Outcomes measured

  • DNA damage assessed by γH2AX (immunofluorescence foci; % γH2AX foci-positive cells; γH2AX expression by western blot)

Limitations

  • In vitro study (cell culture), not human/animal outcomes
  • Sample size not reported in abstract
  • Only γH2AX-based endpoints reported in abstract
View raw extracted JSON
{
  "study_type": "in_vitro",
  "exposure": {
    "band": "ELF",
    "source": null,
    "frequency_mhz": 5e-05,
    "sar_wkg": null,
    "duration": "15 min, 1 h, and 24 h"
  },
  "population": "Human cell lines: human amnion epithelial cells (FLs), human skin fibroblast cells (HSFs), and human umbilical vein endothelial cells (HUVECs)",
  "sample_size": null,
  "outcomes": [
    "DNA damage assessed by γH2AX (immunofluorescence foci; % γH2AX foci-positive cells; γH2AX expression by western blot)"
  ],
  "main_findings": "Cells exposed to 50 Hz ELF-MF at 0.4, 1, and 2 mT for 15 min, 1 h, or 24 h showed no significant differences versus sham in γH2AX foci per cell, percentage of γH2AX foci-positive cells, or γH2AX expression (P > 0.05). The authors conclude no DNA damage was induced, independent of exposure intensity or duration.",
  "effect_direction": "no_effect",
  "limitations": [
    "In vitro study (cell culture), not human/animal outcomes",
    "Sample size not reported in abstract",
    "Only γH2AX-based endpoints reported in abstract"
  ],
  "evidence_strength": "low",
  "confidence": 0.78,
  "peer_reviewed_likely": "yes",
  "keywords": [
    "ELF-MF",
    "50 Hz",
    "magnetic fields",
    "DNA damage",
    "γH2AX",
    "immunofluorescence",
    "western blot",
    "FL cells",
    "HSF cells",
    "HUVECs",
    "0.4 mT",
    "1 mT",
    "2 mT"
  ],
  "suggested_hubs": [
    {
      "slug": "occupational-exposure",
      "weight": 0.2,
      "reason": "Study involves ELF magnetic field exposure levels (mT range), relevant to ELF exposure contexts, though no specific occupational setting is stated."
    }
  ]
}
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AI-extracted fields are generated from the abstract and metadata and may be incomplete or incorrect. This material is for informational purposes and is not medical advice.

Identifiers and source

DOI: 10.1155/2021/8510315
PubMed: Not supplied

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