RF Safe

Evaluation of HSP70 expression and DNA damage in cells of a human trophoblast cell line exposed to 1.8 GHz amplitude-modulated radiofrequency fields

Radiat Res · 2008 · No effect · Low evidence

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No effectLow evidencePaper 6841

In HTR-8/SVneo cells, 1-hour nonthermal exposure to pulse-modulated 1817 MHz RF fields (GSM-217 Hz, SAR 2 W/kg) did not alter HSP70 or HSC70 protein or gene expression. No increase in primary DNA damage or DNA strand breaks was observed after RF exposure.

Study snapshot

PublicationRadiat Res · 2008
Study typein_vitro
Reported directionNo effect
Evidence ratingLow

Abstract

The aim of this study was to determine whether high-frequency electromagnetic fields (EMFs) could induce cellular effects. The human trophoblast cell line HTR-8/SVneo was used as a model to evaluate the expression of proteins (HSP70 and HSC70) and genes (HSP70A, B, C and HSC70) of the HSP70 family and the primary DNA damage response after nonthermal exposure to pulse-modulated 1817 MHz sinusoidal waves (GSM-217 Hz; 1 h; SAR of 2 W/kg). HSP70 expression was significantly enhanced by heat, which was applied as the prototypical stimulus. The HSP70A, B and C transcripts were differentially expressed under basal conditions, and they were all significantly induced above basal levels by thermal stress. Conversely, HSC70 protein and gene expression was not influenced by heat. Exposing HTR-8/SVneo cells to high-frequency EMFs did not change either HSP70 or HSC70 protein or gene expression. A significant increase in DNA strand breaks was caused by exposure to H(2)O(2), which was used as a positive stimulus; however, no effect was observed after exposure of cells to high-frequency EMFs. Overall, no evidence was found that a 1-h exposure to GSM-217 Hz induced a HSP70-mediated stress response or primary DNA damage in HTR-8/SVneo cells. Nevertheless, further investigations on trophoblast cell responses after exposure to GSM signals of different types and durations are needed.

Machine Enhanced Logic

AI evidence extraction

Structured fields below were generated from the paper’s abstract and metadata to make the evidence easier to inspect. Automated extraction may be incomplete or incorrect; verify important details against the original paper.

Study typein_vitro
Effect directionno_effect
PopulationHuman trophoblast cell line HTR-8/SVneo
Sample sizeNot supplied
ExposureRF · 1817 MHz · 2 W/kg · 1 h
Evidence strengthLowConfidence: 97% · Peer reviewed: yes

Main findings

In HTR-8/SVneo cells, 1-hour nonthermal exposure to pulse-modulated 1817 MHz RF fields (GSM-217 Hz, SAR 2 W/kg) did not alter HSP70 or HSC70 protein or gene expression. No increase in primary DNA damage or DNA strand breaks was observed after RF exposure.

Outcomes measured

  • HSP70 protein expression
  • HSC70 protein expression
  • HSP70A gene expression
  • HSP70B gene expression
  • HSP70C gene expression
  • HSC70 gene expression
  • Primary DNA damage
  • DNA strand breaks

Limitations

  • In vitro cell-line study
  • Single exposure duration (1 h)
  • Single frequency/modulation condition (1817 MHz, GSM-217 Hz)
  • Abstract indicates further studies with different signal types and durations are needed
View raw extracted JSON
{
  "study_type": "in_vitro",
  "exposure": {
    "band": "RF",
    "source": "other",
    "frequency_mhz": 1817,
    "sar_wkg": 2,
    "duration": "1 h"
  },
  "population": "Human trophoblast cell line HTR-8/SVneo",
  "sample_size": null,
  "outcomes": [
    "HSP70 protein expression",
    "HSC70 protein expression",
    "HSP70A gene expression",
    "HSP70B gene expression",
    "HSP70C gene expression",
    "HSC70 gene expression",
    "Primary DNA damage",
    "DNA strand breaks"
  ],
  "main_findings": "In HTR-8/SVneo cells, 1-hour nonthermal exposure to pulse-modulated 1817 MHz RF fields (GSM-217 Hz, SAR 2 W/kg) did not alter HSP70 or HSC70 protein or gene expression. No increase in primary DNA damage or DNA strand breaks was observed after RF exposure.",
  "effect_direction": "no_effect",
  "limitations": [
    "In vitro cell-line study",
    "Single exposure duration (1 h)",
    "Single frequency/modulation condition (1817 MHz, GSM-217 Hz)",
    "Abstract indicates further studies with different signal types and durations are needed"
  ],
  "evidence_strength": "low",
  "confidence": 0.97,
  "peer_reviewed_likely": "yes",
  "keywords": [
    "RF",
    "1817 MHz",
    "GSM-217 Hz",
    "SAR 2 W/kg",
    "trophoblast",
    "HTR-8/SVneo",
    "HSP70",
    "HSC70",
    "DNA damage",
    "strand breaks",
    "in vitro",
    "nonthermal exposure"
  ],
  "suggested_hubs": []
}
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AI-extracted fields are generated from the abstract and metadata and may be incomplete or incorrect. This material is for informational purposes and is not medical advice.

Identifiers and source

DOI: 10.1667/rr1061.1
PubMed: 18302482

Read the original paper →

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