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17-β-Estradiol counteracts the effects of high frequency electromagnetic fields on trophoblastic connexins and integrins.

Oxidative medicine and cellular longevity · 2013 · Mixed · Low evidence

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MixedLow evidencePaper 4594

HF-EMF exposure increased Cx40 and Cx43 mRNA expression, decreased integrin alpha1 and beta1 mRNA levels, increased integrin alpha5 mRNA expression, and reduced ER-β mRNA; electron microscopy showed loss of cellular contact in exposed cells. 17-β-estradiol in…

Study snapshot

PublicationOxidative medicine and cellular longevity · 2013
Study typein_vitro
Reported directionMixed
Evidence ratingLow

Abstract

We investigated the effect of high-frequency electromagnetic fields (HF-EMFs) and 17-β-estradiol on connexins (Cxs), integrins (Ints), and estrogen receptor (ER) expression, as well as on ultrastructure of trophoblast-derived HTR-8/SVneo cells. HF-EMF, 17-β-estradiol, and their combination induced an increase of Cx40 and Cx43 mRNA expression. HF-EMF decreased Int alpha1 and β 1 mRNA levels but enhanced Int alpha5 mRNA expression. All the Ints mRNA expressions were increased by 17-β-estradiol and exposure to both stimuli. ER-β mRNA was reduced by HF-EMF but augmented by 17-β-estradiol alone or with HF-EMF. ER-β immunofluorescence showed a cytoplasmic localization in sham and HF-EMF exposed cells which became nuclear after treatment with hormone or both stimuli. Electron microscopy evidenced a loss of cellular contact in exposed cells which appeared counteracted by 17-β-estradiol. We demonstrate that 17-β-estradiol modulates Cxs and Ints as well as ER-β expression induced by HF-EMF, suggesting an influence of both stimuli on trophoblast differentiation and migration.

Machine Enhanced Logic

AI evidence extraction

Structured fields below were generated from the paper’s abstract and metadata to make the evidence easier to inspect. Automated extraction may be incomplete or incorrect; verify important details against the original paper.

Study typein_vitro
Effect directionmixed
PopulationTrophoblast-derived HTR-8/SVneo cells
Sample sizeNot supplied
ExposureRF
Evidence strengthLowConfidence: 74% · Peer reviewed: yes

Main findings

HF-EMF exposure increased Cx40 and Cx43 mRNA expression, decreased integrin alpha1 and beta1 mRNA levels, increased integrin alpha5 mRNA expression, and reduced ER-β mRNA; electron microscopy showed loss of cellular contact in exposed cells. 17-β-estradiol increased integrin mRNA expression and augmented ER-β mRNA, and appeared to counteract HF-EMF-associated loss of cellular contact; combined exposure generally increased Cx and integrin mRNA and promoted nuclear ER-β localization.

Outcomes measured

  • Connexin (Cx40, Cx43) mRNA expression
  • Integrin (alpha1, beta1, alpha5) mRNA expression
  • Estrogen receptor beta (ER-β) mRNA expression
  • ER-β immunofluorescence localization (cytoplasmic vs nuclear)
  • Ultrastructural cellular contact (electron microscopy)

Limitations

  • No exposure parameters reported in abstract (frequency, SAR, duration, source)
  • In vitro cell-line model; generalizability to in vivo pregnancy outcomes is uncertain
  • Sample size and statistical details not provided in abstract
View raw extracted JSON
{
  "study_type": "in_vitro",
  "exposure": {
    "band": "RF",
    "source": null,
    "frequency_mhz": null,
    "sar_wkg": null,
    "duration": null
  },
  "population": "Trophoblast-derived HTR-8/SVneo cells",
  "sample_size": null,
  "outcomes": [
    "Connexin (Cx40, Cx43) mRNA expression",
    "Integrin (alpha1, beta1, alpha5) mRNA expression",
    "Estrogen receptor beta (ER-β) mRNA expression",
    "ER-β immunofluorescence localization (cytoplasmic vs nuclear)",
    "Ultrastructural cellular contact (electron microscopy)"
  ],
  "main_findings": "HF-EMF exposure increased Cx40 and Cx43 mRNA expression, decreased integrin alpha1 and beta1 mRNA levels, increased integrin alpha5 mRNA expression, and reduced ER-β mRNA; electron microscopy showed loss of cellular contact in exposed cells. 17-β-estradiol increased integrin mRNA expression and augmented ER-β mRNA, and appeared to counteract HF-EMF-associated loss of cellular contact; combined exposure generally increased Cx and integrin mRNA and promoted nuclear ER-β localization.",
  "effect_direction": "mixed",
  "limitations": [
    "No exposure parameters reported in abstract (frequency, SAR, duration, source)",
    "In vitro cell-line model; generalizability to in vivo pregnancy outcomes is uncertain",
    "Sample size and statistical details not provided in abstract"
  ],
  "evidence_strength": "low",
  "confidence": 0.74,
  "peer_reviewed_likely": "yes",
  "keywords": [
    "high-frequency electromagnetic fields",
    "HF-EMF",
    "trophoblast",
    "HTR-8/SVneo",
    "connexins",
    "Cx40",
    "Cx43",
    "integrins",
    "estrogen receptor beta",
    "ER-β",
    "17-β-estradiol",
    "ultrastructure",
    "electron microscopy"
  ],
  "suggested_hubs": []
}
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AI-extracted fields are generated from the abstract and metadata and may be incomplete or incorrect. This material is for informational purposes and is not medical advice.

Identifiers and source

DOI: 10.1155/2013/280850
PubMed: 23819010

Read the original paper →

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