[Prevention of Inonotus obliquus polysaccharides for high power microwave radiation induced testicular injury in rats: an experimental research].

Zhongguo Zhong xi yi jie he za zhi Zhongguo Zhongxiyi jiehe zazhi = Chinese journal of integrated traditional and Western medicine · 2014 · Mixed · Low evidence

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MixedLow evidencePaper 6555

Study snapshot

PublicationZhongguo Zhong xi yi jie he za zhi Zhongguo Zhongxiyi jiehe zazhi = Chinese journal of integrated traditional and Western medicine · 2014

Study typeanimal

Reported directionMixed

Evidence ratingLow

Abstract

OBJECTIVE: To investigate the effect of Inonotus obliquus polysaccharides on testicular injury induced by exposure to high power microwave (HPM) in rats. METHODS: A total of 30 male Wistar rats were randomly divided into 5 groups, i.e., the normal control group, the microwave radiation model group, the treatment group, the new microwave radiation model group, and the prevention group, 6 in each group. All rats, except those in the normal control group, were exposed to microwave at an average power density of 200 mW/cm2 for 6 min. Rats in the control group and the model group were administered with normal saline by gastrogavage, once a day. Rats in the treatment group and the prevention group were given with Inonotus obliquus polysaccharides by gastrogavage, 2 mL each time (400 mg/kg body weight), once a day. All rats were sacrificed on the 11th day.The sperm density and the rate of sperm deformity were determined. Pathological changes of testis were observed by light microscope and transmission electron microscope. RESULTS: Short-term HPM irradiation could significantly reduce the sperm density and increase the sperm deformity rate (P < 0.05). Meanwhile, obvious pathological changes of testes occurred. Compared with the two model groups, the sperm density increased and the sperm deformity rate decreased in the treatment group and the prevention group (P < 0.05). Under the light microscope, injuries of spermatogenic cells and stromal cells, as well as vascular dilatation and congestion were obviously alleviated in the treatment group and the prevention group. Mitochondrial swelling and endoplasmic reticulum expansion shown by ultrastructural observation were also significantly alleviated. Of them, injuries of spermatogenic cells and inflammation response were milder in the treatment group than in the prevention group. CONCLUSIONS: Inonotus obliquus polysaccharides had significant protective effect on microwave radiation induced testicular injury. Better effect was obtained by therapeutic medication than preventive medication.

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Study typeanimal

Effect directionmixed

PopulationMale Wistar rats

Sample size30

Exposuremicrowave · 6 min

Evidence strengthLowConfidence: 78% · Peer reviewed: yes

Main findings

Short-term high power microwave irradiation (average power density 200 mW/cm2 for 6 min) reduced sperm density and increased sperm deformity rate, with obvious testicular pathological changes. Compared with model groups, Inonotus obliquus polysaccharides (400 mg/kg daily by gavage) increased sperm density and decreased sperm deformity rate and alleviated histopathological and ultrastructural injuries; therapeutic administration showed milder spermatogenic cell injury and inflammation than preventive administration.

Outcomes measured

Sperm density
Sperm deformity rate
Testicular pathology (light microscopy)
Testicular ultrastructure (transmission electron microscopy)

Limitations

Frequency of microwave exposure not reported
SAR not reported
Small group sizes (6 rats per group)
Follow-up limited to sacrifice on day 11

View raw extracted JSON

{
  "study_type": "animal",
  "exposure": {
    "band": "microwave",
    "source": null,
    "frequency_mhz": null,
    "sar_wkg": null,
    "duration": "6 min"
  },
  "population": "Male Wistar rats",
  "sample_size": 30,
  "outcomes": [
    "Sperm density",
    "Sperm deformity rate",
    "Testicular pathology (light microscopy)",
    "Testicular ultrastructure (transmission electron microscopy)"
  ],
  "main_findings": "Short-term high power microwave irradiation (average power density 200 mW/cm2 for 6 min) reduced sperm density and increased sperm deformity rate, with obvious testicular pathological changes. Compared with model groups, Inonotus obliquus polysaccharides (400 mg/kg daily by gavage) increased sperm density and decreased sperm deformity rate and alleviated histopathological and ultrastructural injuries; therapeutic administration showed milder spermatogenic cell injury and inflammation than preventive administration.",
  "effect_direction": "mixed",
  "limitations": [
    "Frequency of microwave exposure not reported",
    "SAR not reported",
    "Small group sizes (6 rats per group)",
    "Follow-up limited to sacrifice on day 11"
  ],
  "evidence_strength": "low",
  "confidence": 0.78,
  "peer_reviewed_likely": "yes",
  "keywords": [
    "high power microwave",
    "microwave radiation",
    "testicular injury",
    "sperm density",
    "sperm deformity",
    "Inonotus obliquus polysaccharides",
    "rat",
    "protective effect"
  ],
  "suggested_hubs": [
    {
      "slug": "animal-studies",
      "weight": 0.9,
      "reason": "Experimental rat study of microwave exposure and reproductive/testicular outcomes."
    }
  ]
}

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AI-extracted fields are generated from the abstract and metadata and may be incomplete or incorrect. This material is for informational purposes and is not medical advice.

Identifiers and source

DOI: Not supplied
PubMed: 25137855

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