Effects of differently polarized microwave radiation on the microscopic structure of the nuclei in human fibroblasts.

Journal of Zhejiang University. Science. B · 2010 · Mixed · Low evidence

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MixedLow evidencePaper 6502

At 36.65 GHz for 10 s, exposure at 1 µW/cm(2) produced no effect on heterochromatin granule quantity (HGQ), while 10 and 100 µW/cm(2) increased HGQ, with more intense irradiation associated with more chromatin condensation. Right-handed elliptically polarized…

Study snapshot

PublicationJournal of Zhejiang University. Science. B · 2010

Study typein_vitro

Reported directionMixed

Evidence ratingLow

Abstract

To investigate the influence of microwave radiation on the human fibroblast nuclei, the effects of three variants of electromagnetic wave polarization, linear and left-handed and right-handed elliptically polarized, were examined. Experimental conditions were: frequency (f) 36.65 GHz, power density (P) at the surface of exposed object 1, 10, 30, and 100 µW/cm(2), exposure time 10 s. Human fibroblasts growing in a monolayer on a cover slide were exposed to microwave electromagnetic radiation. The layer of medium that covered cells during microwave exposure was about 1 mm thick. Cells were stained immediately after irradiation by 2% (w/v) orcein solution in 45% (w/v) acetic acid. Experiments were made at room temperature (25 °C), and control cell samples were processed in the same conditions. We assessed heterochromatin granule quantity (HGQ) at 600× magnification. Microwave irradiation at the intensity of 1 µW/cm(2) produced no effect, and irradiation at the intensities of 10 and 100 µW/cm(2) induced an increase in HGQ. More intense irradiation induced more chromatin condensation. The right-handed elliptically polarized radiation revealed more biological activity than the left-handed polarized one.

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AI evidence extraction

Structured fields below were generated from the paper’s abstract and metadata to make the evidence easier to inspect. Automated extraction may be incomplete or incorrect; verify important details against the original paper.

Study typein_vitro

Effect directionmixed

PopulationHuman fibroblasts (monolayer culture)

Sample sizeNot supplied

ExposuremmWave · 36650 MHz · 10 s

Evidence strengthLowConfidence: 78% · Peer reviewed: yes

Main findings

Outcomes measured

Heterochromatin granule quantity (HGQ)
Chromatin condensation / microscopic nuclear structure

Limitations

In vitro study (human fibroblast culture)
Sample size not reported in abstract
Only immediate post-irradiation assessment reported
Exposure metric reported as power density; SAR not reported
Short exposure duration (10 s)

View raw extracted JSON

{
  "study_type": "in_vitro",
  "exposure": {
    "band": "mmWave",
    "source": null,
    "frequency_mhz": 36650,
    "sar_wkg": null,
    "duration": "10 s"
  },
  "population": "Human fibroblasts (monolayer culture)",
  "sample_size": null,
  "outcomes": [
    "Heterochromatin granule quantity (HGQ)",
    "Chromatin condensation / microscopic nuclear structure"
  ],
  "main_findings": "At 36.65 GHz for 10 s, exposure at 1 µW/cm(2) produced no effect on heterochromatin granule quantity (HGQ), while 10 and 100 µW/cm(2) increased HGQ, with more intense irradiation associated with more chromatin condensation. Right-handed elliptically polarized radiation showed more biological activity than left-handed elliptically polarized radiation.",
  "effect_direction": "mixed",
  "limitations": [
    "In vitro study (human fibroblast culture)",
    "Sample size not reported in abstract",
    "Only immediate post-irradiation assessment reported",
    "Exposure metric reported as power density; SAR not reported",
    "Short exposure duration (10 s)"
  ],
  "evidence_strength": "low",
  "confidence": 0.78,
  "peer_reviewed_likely": "yes",
  "keywords": [
    "microwave radiation",
    "millimeter wave",
    "36.65 GHz",
    "polarization",
    "linear polarization",
    "elliptical polarization",
    "human fibroblasts",
    "heterochromatin granules",
    "chromatin condensation",
    "HGQ",
    "power density"
  ],
  "suggested_hubs": []
}

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AI-extracted fields are generated from the abstract and metadata and may be incomplete or incorrect. This material is for informational purposes and is not medical advice.

Identifiers and source

DOI: 10.1631/jzus.b1000051
PubMed: 20872988

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