Electromagnetic field-mediated chitosan/gelatin/nano-hydroxyapatite and bone-derived scaffolds regulate the osteoblastic and chondrogenic phenotypes of adipose-derived stem cells to construct osteochondral tissue engineering niche in vitro.

International journal of biological macromolecules · 2024 · Mixed · Insufficient evidence

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MixedInsufficient evidencePaper 5761

Study snapshot

PublicationInternational journal of biological macromolecules · 2024

Study typein_vitro

Reported directionMixed

Evidence ratingInsufficient

Abstract

It is critical to explore the effects of electromagnetic field (EMF) on the construction of functional osteochondral tissue, which has shown certain clinical significance for the treatment of osteochondral injury. At present, there are few studies on the effect of the direction of EMF on cells. This study aimed to investigate the effects of EMF coupling on different parameters to control adipose-derived stem cells (ADSCs) proliferation and specific chondrogenic and osteogenic differentiation at 2D level and 3D level. The proliferation and differentiation of EMF-induced ADSCs are jointly regulated by EMF and space structure. In this study, Cs/Gel/nHAP scaffolds were prepared with good degradation rate (86.75 ± 4.96 %) and absorb water (1100 %), and the pore size was 195.63 ± 54.72 μm. The bone-derived scaffold with a pore size of 267.17 ± 129.18 μm was obtained and its main component was hydroxyapatite. Cs/Gel/nHAP scaffolds and bone-derived scaffolds are suitable as 3D level materials. The optimal EMF intensity was 2 mT for chondrogenic differentiation and proliferation and 1 mT for osteogenic differentiation and proliferation. It is noteworthy that EMF has a negative correlation with ADSCs proliferation in the vertical direction at 2D level, while it has a positive correlation with ADSCs proliferation at 3D level. EMF mediated 3D osteochondral scaffold provide good strategy for osteochondral tissue engineering construction.

Machine Enhanced Logic

AI evidence extraction

Structured fields below were generated from the paper’s abstract and metadata to make the evidence easier to inspect. Automated extraction may be incomplete or incorrect; verify important details against the original paper.

Study typein_vitro

Effect directionmixed

PopulationAdipose-derived stem cells (ADSCs) in 2D and 3D culture; chitosan/gelatin/nano-hydroxyapatite (Cs/Gel/nHAP) scaffolds and bone-derived scaffolds

Sample sizeNot supplied

ExposureNot supplied

Evidence strengthInsufficientConfidence: 74% · Peer reviewed: yes

Main findings

The study reports optimal EMF intensities of 2 mT for chondrogenic differentiation/proliferation and 1 mT for osteogenic differentiation/proliferation. EMF showed a negative correlation with ADSC proliferation in the vertical direction at the 2D level, but a positive correlation with ADSC proliferation at the 3D level; effects were described as jointly regulated by EMF and spatial structure.

Outcomes measured

ADSC proliferation
Chondrogenic differentiation
Osteogenic differentiation
Scaffold properties (degradation rate, water absorption, pore size)
Effect of EMF direction (vertical) on proliferation in 2D vs 3D

Limitations

Frequency, waveform, and exposure duration were not reported in the abstract.
Sample size and statistical details were not provided in the abstract.
Findings are from in vitro 2D/3D models; clinical relevance cannot be determined from the abstract alone.

View raw extracted JSON

{
  "study_type": "in_vitro",
  "exposure": {
    "band": null,
    "source": "other",
    "frequency_mhz": null,
    "sar_wkg": null,
    "duration": null
  },
  "population": "Adipose-derived stem cells (ADSCs) in 2D and 3D culture; chitosan/gelatin/nano-hydroxyapatite (Cs/Gel/nHAP) scaffolds and bone-derived scaffolds",
  "sample_size": null,
  "outcomes": [
    "ADSC proliferation",
    "Chondrogenic differentiation",
    "Osteogenic differentiation",
    "Scaffold properties (degradation rate, water absorption, pore size)",
    "Effect of EMF direction (vertical) on proliferation in 2D vs 3D"
  ],
  "main_findings": "The study reports optimal EMF intensities of 2 mT for chondrogenic differentiation/proliferation and 1 mT for osteogenic differentiation/proliferation. EMF showed a negative correlation with ADSC proliferation in the vertical direction at the 2D level, but a positive correlation with ADSC proliferation at the 3D level; effects were described as jointly regulated by EMF and spatial structure.",
  "effect_direction": "mixed",
  "limitations": [
    "Frequency, waveform, and exposure duration were not reported in the abstract.",
    "Sample size and statistical details were not provided in the abstract.",
    "Findings are from in vitro 2D/3D models; clinical relevance cannot be determined from the abstract alone."
  ],
  "evidence_strength": "insufficient",
  "confidence": 0.74,
  "peer_reviewed_likely": "yes",
  "keywords": [
    "electromagnetic field",
    "EMF",
    "adipose-derived stem cells",
    "ADSC",
    "chondrogenic differentiation",
    "osteogenic differentiation",
    "osteochondral tissue engineering",
    "3D scaffold",
    "chitosan",
    "gelatin",
    "nano-hydroxyapatite",
    "bone-derived scaffold",
    "mT"
  ],
  "suggested_hubs": []
}

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AI-extracted fields are generated from the abstract and metadata and may be incomplete or incorrect. This material is for informational purposes and is not medical advice.

Identifiers and source

DOI: 10.1016/j.ijbiomac.2023.128829
PubMed: 38128807

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