Low-frequency electromagnetic field influences human oral mucosa keratinocyte viability in response to lipopolysaccharide or minocycline treatment in cell culture conditions.

Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie · 2021 · Mixed · Low evidence

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MixedLow evidencePaper 5707

Exposure of human oral keratinocyte cultures to low-frequency electromagnetic field (LF-EMF), alone or combined with lipopolysaccharide (LPS) or minocycline, caused changes in the percentages of cells undergoing programmed (apoptosis) or incidental (necrosis)…

Study snapshot

PublicationBiomedicine & pharmacotherapy = Biomedecine & pharmacotherapie · 2021

Study typein_vitro

Reported directionMixed

Evidence ratingLow

Abstract

The aim of the current study was to investigate the influence of low-frequency electromagnetic field (LF-EMF) exposure on viability parameters of oral mucosa keratinocytes cultured in in vitro conditions. The effect of LF-EMF stimulation on cell viability was also specified in the simultaneous presence of lipopolysaccharide (LPS) infectious agent or minocycline (Mino) anti-inflammatory agent. Viability parameters such as early-, late apoptosis and necrosis of keratinocytes were analysed by the flow cytometry method (FCM). The exposure of human oral keratinocyte cell cultures to LF-EMF acting alone or combined with LPS/minocycline agents caused changes in the percentage of cells that undergo programmed or incidental cell death. The overall obtained results are compiled in a graphical form presented in Fig. 1.

Machine Enhanced Logic

AI evidence extraction

Structured fields below were generated from the paper’s abstract and metadata to make the evidence easier to inspect. Automated extraction may be incomplete or incorrect; verify important details against the original paper.

Study typein_vitro

Effect directionmixed

PopulationHuman oral mucosa keratinocytes (cell culture)

Sample sizeNot supplied

ExposureELF

Evidence strengthLowConfidence: 66% · Peer reviewed: yes

Main findings

Outcomes measured

Keratinocyte viability parameters
Early apoptosis
Late apoptosis
Necrosis

Limitations

In vitro cell culture study (no in vivo/clinical outcomes reported in abstract)
LF-EMF exposure characteristics (e.g., frequency, intensity, duration) not provided in abstract
Numerical results and direction of changes not reported in abstract (referenced as compiled in Fig. 1)
Sample size not reported in abstract

View raw extracted JSON

{
  "study_type": "in_vitro",
  "exposure": {
    "band": "ELF",
    "source": null,
    "frequency_mhz": null,
    "sar_wkg": null,
    "duration": null
  },
  "population": "Human oral mucosa keratinocytes (cell culture)",
  "sample_size": null,
  "outcomes": [
    "Keratinocyte viability parameters",
    "Early apoptosis",
    "Late apoptosis",
    "Necrosis"
  ],
  "main_findings": "Exposure of human oral keratinocyte cultures to low-frequency electromagnetic field (LF-EMF), alone or combined with lipopolysaccharide (LPS) or minocycline, caused changes in the percentages of cells undergoing programmed (apoptosis) or incidental (necrosis) cell death.",
  "effect_direction": "mixed",
  "limitations": [
    "In vitro cell culture study (no in vivo/clinical outcomes reported in abstract)",
    "LF-EMF exposure characteristics (e.g., frequency, intensity, duration) not provided in abstract",
    "Numerical results and direction of changes not reported in abstract (referenced as compiled in Fig. 1)",
    "Sample size not reported in abstract"
  ],
  "evidence_strength": "low",
  "confidence": 0.66,
  "peer_reviewed_likely": "yes",
  "keywords": [
    "low-frequency electromagnetic field",
    "LF-EMF",
    "oral mucosa",
    "keratinocytes",
    "cell viability",
    "apoptosis",
    "necrosis",
    "lipopolysaccharide",
    "minocycline",
    "flow cytometry"
  ],
  "suggested_hubs": []
}

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AI-extracted fields are generated from the abstract and metadata and may be incomplete or incorrect. This material is for informational purposes and is not medical advice.

Identifiers and source

DOI: 10.1016/j.biopha.2021.111340
PubMed: 33556878

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