The Dynamic(s) of Adipose Stem Cell System, Their Survival, and Cessation under the Influence of Electromagnetic Fields.

Journal of medical physics · 2021 · Mixed · Low evidence

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MixedLow evidencePaper 5670

Continuous exposure of adipose-derived stem cells to EMF set to ion cyclotron resonance parameters (76.6 Hz; 20 μT) was associated with a statistically significant increase in cell death, with increased apoptotic, necrotic, and autophagic cell numbers. Increa…

Study snapshot

PublicationJournal of medical physics · 2021

Study typein_vitro

Reported directionMixed

Evidence ratingLow

Abstract

CONTEXT: The electromagnetic field (EMF) is one of the external biophysical factors that can influence stem cells' structure and functionality. Depending on its frequency and magnetic flux density, EMF can have both a positive and negative effect on stem cell biology. AIMS: The aim of the study is to define EMF conditions that support beneficial physiological processes and those that lead to pathophysiological phenomena. Understanding the changes and processes occurring in stem cells after exposure to EMFs of different parameters can be an important factor to be applied in stem cell-based therapies and regenerative medicine. MATERIALS AND METHODS: In this study, using fluorescent microscopy and flow cytometry methods, the influence of EMF on adipose-derived stem cells proliferation, cell cycle, viability, and death were examined. EMF parameters were set in accordance with the ion cyclotron resonance (ICR) theory that influences Ca and Mg ions influx. Results were statistically developed using the ANOVA and effect size (Cohen's d) analyses. RESULTS: In this study, the continuous exposure of adipose-derived stem cells to EMF (ICR parameters: 76.6 Hz; 20 μT) causes a statistically significant increase in cell death through the enhancement of apoptotic, necrotic, and autophagic cell numbers. Apart from increased cell deaths after EMF exposure, increased proliferation after 24 h of EMF exposure has been also observed. CONCLUSIONS: Results presented in this study show that EMF influences stem cell dynamics resulting in a significantly increased cell death, thus altering the stem cell fate. It is important to further establish EMF conditions that support ASCs functioning and beneficial physiological processes for future regenerative medical purposes.

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AI evidence extraction

Structured fields below were generated from the paper’s abstract and metadata to make the evidence easier to inspect. Automated extraction may be incomplete or incorrect; verify important details against the original paper.

Study typein_vitro

Effect directionmixed

PopulationAdipose-derived stem cells (ASCs)

Sample sizeNot supplied

ExposureELF · continuous; 24 h mentioned for proliferation observation

Evidence strengthLowConfidence: 78% · Peer reviewed: yes

Main findings

Outcomes measured

cell proliferation
cell cycle
cell viability
cell death
apoptosis
necrosis
autophagy

Limitations

Sample size not reported in abstract
Exposure duration and full exposure protocol details not fully specified in abstract
In vitro study; generalizability to in vivo/clinical settings not addressed in abstract

View raw extracted JSON

{
  "study_type": "in_vitro",
  "exposure": {
    "band": "ELF",
    "source": null,
    "frequency_mhz": null,
    "sar_wkg": null,
    "duration": "continuous; 24 h mentioned for proliferation observation"
  },
  "population": "Adipose-derived stem cells (ASCs)",
  "sample_size": null,
  "outcomes": [
    "cell proliferation",
    "cell cycle",
    "cell viability",
    "cell death",
    "apoptosis",
    "necrosis",
    "autophagy"
  ],
  "main_findings": "Continuous exposure of adipose-derived stem cells to EMF set to ion cyclotron resonance parameters (76.6 Hz; 20 μT) was associated with a statistically significant increase in cell death, with increased apoptotic, necrotic, and autophagic cell numbers. Increased proliferation after 24 h of EMF exposure was also observed.",
  "effect_direction": "mixed",
  "limitations": [
    "Sample size not reported in abstract",
    "Exposure duration and full exposure protocol details not fully specified in abstract",
    "In vitro study; generalizability to in vivo/clinical settings not addressed in abstract"
  ],
  "evidence_strength": "low",
  "confidence": 0.78,
  "peer_reviewed_likely": "yes",
  "keywords": [
    "electromagnetic field",
    "ELF-EMF",
    "ion cyclotron resonance",
    "76.6 Hz",
    "20 μT",
    "adipose-derived stem cells",
    "proliferation",
    "apoptosis",
    "necrosis",
    "autophagy",
    "cell viability",
    "flow cytometry",
    "fluorescent microscopy"
  ],
  "suggested_hubs": []
}

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AI-extracted fields are generated from the abstract and metadata and may be incomplete or incorrect. This material is for informational purposes and is not medical advice.

Identifiers and source

DOI: 10.4103/jmp.jmp_10_21
PubMed: 34703098

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