Electromagnetic Field Could Protect SH-SY5Y Cells Against Cisplatin Cytotoxicity, But Not MCF-7 Cells.

DNA and cell biology · 2018 · Mixed · Very low evidence

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MixedVery low evidencePaper 5555

Study snapshot

PublicationDNA and cell biology · 2018

Study typein_vitro

Reported directionMixed

Evidence ratingVery low

Abstract

Cisplatin [cis-dichlorodiammine platinum (II), CDDP], morphine (Mor), and electromagnetic field (EMF) induced oxidative stress. In this study, we tried to increase the cytotoxicity of CDDP in combination with Mor and/or EMF in MCF-7 and SH-SY5Y cells. Furthermore, we evaluate the expression levels of 11 antioxidant genes in both cell lines. We designed four treatments: CDDP alone, "CDDP+Mor," "CDDP+EMF," and "CDDP+Mor+EMF." Serial dilutions of CDDP, Mor (5.0 μM), and EMF (50 Hz, 0.50 mT, "15 min field-on/15 min field-off") were used for estimation of relative IC values. The mRNA expression levels of antioxidant genes were determined by real-time PCR. The IC value of CDDP in "CDDP+Mor+EMF" treatment was significantly higher than CDDP alone and "CDDP+Mor" treatments in both cell lines. Whereas the expression levels of antioxidant genes in the four treatments showed similar patterns in MCF-7 cells, in SH-SY5Y cells, most of the antioxidant genes showed an upregulation with "CDDP+EMF" and "CDDP+Mor+EMF" treatments. Moreover, significant differences in the number of upregulated genes were observed between different treatments in SH-SY5Y cells. The molecular mechanism of CDDP-reduced cytotoxicity in our designed combinations is probably different in MCF-7 and SH-SY5Y cells. CDDP in combination with EMF could protect SH-SY5Y cells from the cytotoxicity, whereas it has no significant change in MCF-7 cells.

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AI evidence extraction

Structured fields below were generated from the paper’s abstract and metadata to make the evidence easier to inspect. Automated extraction may be incomplete or incorrect; verify important details against the original paper.

Study typein_vitro

Effect directionmixed

PopulationMCF-7 and SH-SY5Y cell lines

Sample sizeNot supplied

ExposureELF · 15 min field-on/15 min field-off

Evidence strengthVery lowConfidence: 78% · Peer reviewed: yes

Main findings

In both MCF-7 and SH-SY5Y cells, the IC value of cisplatin in the CDDP+Mor+EMF condition was significantly higher than with CDDP alone and CDDP+Mor. In SH-SY5Y cells, most antioxidant genes were upregulated with CDDP+EMF and CDDP+Mor+EMF, while MCF-7 showed similar antioxidant gene expression patterns across treatments. The authors report that EMF in combination with CDDP could protect SH-SY5Y cells from cytotoxicity, with no significant change in MCF-7 cells.

Outcomes measured

Cisplatin (CDDP) cytotoxicity (IC values)
mRNA expression levels of 11 antioxidant genes (real-time PCR)

Limitations

In vitro study (cell lines), not directly generalizable to humans
Sample size/replicates not reported in abstract
Exposure details limited to 50 Hz, 0.50 mT and duty cycle; total exposure duration not specified
Only antioxidant gene mRNA expression assessed (no protein/activity measures described in abstract)

View raw extracted JSON

{
  "study_type": "in_vitro",
  "exposure": {
    "band": "ELF",
    "source": "other",
    "frequency_mhz": null,
    "sar_wkg": null,
    "duration": "15 min field-on/15 min field-off"
  },
  "population": "MCF-7 and SH-SY5Y cell lines",
  "sample_size": null,
  "outcomes": [
    "Cisplatin (CDDP) cytotoxicity (IC values)",
    "mRNA expression levels of 11 antioxidant genes (real-time PCR)"
  ],
  "main_findings": "In both MCF-7 and SH-SY5Y cells, the IC value of cisplatin in the CDDP+Mor+EMF condition was significantly higher than with CDDP alone and CDDP+Mor. In SH-SY5Y cells, most antioxidant genes were upregulated with CDDP+EMF and CDDP+Mor+EMF, while MCF-7 showed similar antioxidant gene expression patterns across treatments. The authors report that EMF in combination with CDDP could protect SH-SY5Y cells from cytotoxicity, with no significant change in MCF-7 cells.",
  "effect_direction": "mixed",
  "limitations": [
    "In vitro study (cell lines), not directly generalizable to humans",
    "Sample size/replicates not reported in abstract",
    "Exposure details limited to 50 Hz, 0.50 mT and duty cycle; total exposure duration not specified",
    "Only antioxidant gene mRNA expression assessed (no protein/activity measures described in abstract)"
  ],
  "evidence_strength": "very_low",
  "confidence": 0.78,
  "peer_reviewed_likely": "yes",
  "keywords": [
    "ELF-EMF",
    "50 Hz",
    "0.50 mT",
    "cisplatin",
    "morphine",
    "MCF-7",
    "SH-SY5Y",
    "oxidative stress",
    "antioxidant genes",
    "cytotoxicity",
    "IC value"
  ],
  "suggested_hubs": []
}

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AI-extracted fields are generated from the abstract and metadata and may be incomplete or incorrect. This material is for informational purposes and is not medical advice.

Identifiers and source

DOI: 10.1089/dna.2017.4108
PubMed: 29446648

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