Expressions of some antioxidant genes in SH-SY5Y cells treated with β-lapachone, morphine and electromagnetic field.

Molecular biology reports · 2018 · Mixed · Low evidence

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MixedLow evidencePaper 5545

Study snapshot

PublicationMolecular biology reports · 2018

Study typein_vitro

Reported directionMixed

Evidence ratingLow

Abstract

β-Lapachone (β-Lap), morphine (Mor), and electromagnetic field (EMF) generate reactive oxygen species. The goal of the present study was to examine the effects of Mor and EMF, in combination with β-Lap on the cell growth inhibition and expression of several antioxidant genes. The 0.50 mT intensity of 50 Hz EMF and two exposure conditions ("15 min field-on/15 min field-off" and "30 min field-on continuously") on SH-SY5Y cells were used. The effects of Mor and EMF, in combination with β-Lap on cell growth inhibition and the expression levels of several antioxidant genes (NQO1, NQO2, SOD1, SOD2, CAT, GSTO1, GSTM2, GSTM3, GSTP1, MGST1, MGST3) in SH-SY5Y cells were measured. The relative mRNA levels were calculated according to the [Formula: see text]. Whereas NQO1 mRNA level decreased in the "15 min field-on/15 min field-off" condition, the expression level of NQO2 was increased. Both NQO1 and NQO2 expressions increased in Mor treated cells. IC values of β-Lap in combination with Mor, EMF, and "Mor + EMF" were higher than cells treated only with β-Lap. The NQO1 expression level in the cells treated with β-Lap was higher than the other treatments, indicating that β-Lap induces the expression of NQO1. Moreover, multiple linear regression analysis indicated that NQO1 mRNA levels were associated positively with β-Lap and negatively with EMF. At least in part, the mRNA levels of NQO1 were associated with IC values of β-Lap in designed treatments. There is a negative association between mRNA levels of NQO1 and IC values of β-Lap but not NQO2.

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AI evidence extraction

Structured fields below were generated from the paper’s abstract and metadata to make the evidence easier to inspect. Automated extraction may be incomplete or incorrect; verify important details against the original paper.

Study typein_vitro

Effect directionmixed

PopulationSH-SY5Y cells

Sample sizeNot supplied

ExposureELF · 0.05 MHz · Two exposure conditions: "15 min field-on/15 min field-off" and "30 min field-on continuously"

Evidence strengthLowConfidence: 78% · Peer reviewed: yes

Main findings

Using a 0.50 mT, 50 Hz EMF, NQO1 mRNA decreased under the "15 min field-on/15 min field-off" condition while NQO2 increased. Multiple linear regression indicated NQO1 mRNA levels were positively associated with β-lapachone and negatively associated with EMF; IC values of β-lapachone in combination with morphine, EMF, or morphine+EMF were higher than with β-lapachone alone.

Outcomes measured

Cell growth inhibition (IC values of β-lapachone)
mRNA expression of antioxidant genes (NQO1, NQO2, SOD1, SOD2, CAT, GSTO1, GSTM2, GSTM3, GSTP1, MGST1, MGST3)

Limitations

In vitro model (SH-SY5Y cells) may not generalize to in vivo/human outcomes
Sample size not reported in abstract
Exposure duration beyond the stated on/off patterns not specified
Outcomes limited to mRNA expression and cell growth inhibition in this experimental context

View raw extracted JSON

{
  "study_type": "in_vitro",
  "exposure": {
    "band": "ELF",
    "source": "other",
    "frequency_mhz": 0.05,
    "sar_wkg": null,
    "duration": "Two exposure conditions: \"15 min field-on/15 min field-off\" and \"30 min field-on continuously\""
  },
  "population": "SH-SY5Y cells",
  "sample_size": null,
  "outcomes": [
    "Cell growth inhibition (IC values of β-lapachone)",
    "mRNA expression of antioxidant genes (NQO1, NQO2, SOD1, SOD2, CAT, GSTO1, GSTM2, GSTM3, GSTP1, MGST1, MGST3)"
  ],
  "main_findings": "Using a 0.50 mT, 50 Hz EMF, NQO1 mRNA decreased under the \"15 min field-on/15 min field-off\" condition while NQO2 increased. Multiple linear regression indicated NQO1 mRNA levels were positively associated with β-lapachone and negatively associated with EMF; IC values of β-lapachone in combination with morphine, EMF, or morphine+EMF were higher than with β-lapachone alone.",
  "effect_direction": "mixed",
  "limitations": [
    "In vitro model (SH-SY5Y cells) may not generalize to in vivo/human outcomes",
    "Sample size not reported in abstract",
    "Exposure duration beyond the stated on/off patterns not specified",
    "Outcomes limited to mRNA expression and cell growth inhibition in this experimental context"
  ],
  "evidence_strength": "low",
  "confidence": 0.78,
  "peer_reviewed_likely": "yes",
  "keywords": [
    "ELF-EMF",
    "50 Hz",
    "0.50 mT",
    "SH-SY5Y",
    "β-lapachone",
    "morphine",
    "reactive oxygen species",
    "antioxidant genes",
    "NQO1",
    "NQO2",
    "gene expression",
    "cell growth inhibition"
  ],
  "suggested_hubs": []
}

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AI-extracted fields are generated from the abstract and metadata and may be incomplete or incorrect. This material is for informational purposes and is not medical advice.

Identifiers and source

DOI: 10.1007/s11033-018-4172-1
PubMed: 29611025

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