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Pre-exposure of neuroblastoma cell line to pulsed electromagnetic field prevents H2 O2 -induced ROS production by increasing MnSOD activity.

Bioelectromagnetics · 2015 · Benefit · Low evidence

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BenefitLow evidencePaper 4792

A short, repeated PEMF pre-exposure (40 min total; 4×/week, 10 min each) did not affect major physiological parameters and significantly increased MnSOD activity in SH-SY5Y cells. PEMF-pre-exposed cells showed decreased ROS production after a 30 min H2O2 chal…

Study snapshot

PublicationBioelectromagnetics · 2015
Study typein_vitro
Reported directionBenefit
Evidence ratingLow

Abstract

Electromagnetic fields (EMFs) have been linked to increased risk of cancers and neurodegenerative diseases; however, EMFs can also elicit positive effects on biological systems, and redox status seems crucially involved in EMF biological effects. This study aimed to assess whether a short and repeated pulsed EMF (PEMF) could trigger adaptive responses against an oxidative insult in a neuronal cellular model. We found that a 40 min overall (four times a week, 10 min each) pre-exposure to PEMF did not affect major physiological parameters and led to a significant increase of Mn-dependent superoxide dismutase activity in the human neuroblastoma SH-SY5Y cell line. In addition, we found PEMF-pre-exposed cells exhibited decreased reactive oxygen species production following a 30 min H2 O2 challenge, with respect to non pre-exposed cells. Our findings might provide new insights on the role played by short and repeated PEMF stimulations in the enhancement of cellular defenses against oxidative insults. Although studies in normal neuronal cells would be useful to further confirm our hypothesis, we suggest that specific PEMF treatments may have potential biological repercussions in diseases where oxidative stress is implicated.

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AI evidence extraction

Structured fields below were generated from the paper’s abstract and metadata to make the evidence easier to inspect. Automated extraction may be incomplete or incorrect; verify important details against the original paper.

Study typein_vitro
Effect directionbenefit
PopulationHuman neuroblastoma SH-SY5Y cell line
Sample sizeNot supplied
Exposure40 min overall (four times a week, 10 min each) pre-exposure; oxidative challenge: 30 min H2O2
Evidence strengthLowConfidence: 74% · Peer reviewed: yes

Main findings

A short, repeated PEMF pre-exposure (40 min total; 4×/week, 10 min each) did not affect major physiological parameters and significantly increased MnSOD activity in SH-SY5Y cells. PEMF-pre-exposed cells showed decreased ROS production after a 30 min H2O2 challenge compared with non-pre-exposed cells.

Outcomes measured

  • Mn-dependent superoxide dismutase (MnSOD) activity
  • Reactive oxygen species (ROS) production after H2O2 challenge
  • Major physiological parameters (not specified)

Limitations

  • In vitro study in a neuroblastoma cell line (SH-SY5Y), not normal neuronal cells
  • PEMF exposure parameters (e.g., frequency, intensity) not provided in the abstract
  • Sample size and detailed methods not reported in the abstract
View raw extracted JSON
{
  "study_type": "in_vitro",
  "exposure": {
    "band": null,
    "source": "pulsed electromagnetic field (PEMF)",
    "frequency_mhz": null,
    "sar_wkg": null,
    "duration": "40 min overall (four times a week, 10 min each) pre-exposure; oxidative challenge: 30 min H2O2"
  },
  "population": "Human neuroblastoma SH-SY5Y cell line",
  "sample_size": null,
  "outcomes": [
    "Mn-dependent superoxide dismutase (MnSOD) activity",
    "Reactive oxygen species (ROS) production after H2O2 challenge",
    "Major physiological parameters (not specified)"
  ],
  "main_findings": "A short, repeated PEMF pre-exposure (40 min total; 4×/week, 10 min each) did not affect major physiological parameters and significantly increased MnSOD activity in SH-SY5Y cells. PEMF-pre-exposed cells showed decreased ROS production after a 30 min H2O2 challenge compared with non-pre-exposed cells.",
  "effect_direction": "benefit",
  "limitations": [
    "In vitro study in a neuroblastoma cell line (SH-SY5Y), not normal neuronal cells",
    "PEMF exposure parameters (e.g., frequency, intensity) not provided in the abstract",
    "Sample size and detailed methods not reported in the abstract"
  ],
  "evidence_strength": "low",
  "confidence": 0.74,
  "peer_reviewed_likely": "yes",
  "keywords": [
    "pulsed electromagnetic field",
    "PEMF",
    "SH-SY5Y",
    "neuroblastoma",
    "oxidative stress",
    "H2O2",
    "reactive oxygen species",
    "ROS",
    "MnSOD",
    "adaptive response"
  ],
  "suggested_hubs": []
}
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AI-extracted fields are generated from the abstract and metadata and may be incomplete or incorrect. This material is for informational purposes and is not medical advice.

Identifiers and source

DOI: 10.1002/bem.21900
PubMed: 25708841

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