Low frequency electromagnetic field decreases ischemia-reperfusion injury of human cardiomyocytes and supports their metabolic function.

Experimental biology and medicine (Maywood, N.J.) · 2018 · Benefit · Low evidence

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BenefitLow evidencePaper 5535

Study snapshot

PublicationExperimental biology and medicine (Maywood, N.J.) · 2018

Study typein_vitro

Reported directionBenefit

Evidence ratingLow

Abstract

Electromagnetic field at extremely low frequencies plays a significant role in the physiological function of human tissues and systems. It is shown that electromagnetic field inhibits production of reactive oxygen species which are involved in heart injury triggered by oxidative stress. We hypothesize that low frequency electromagnetic field protects function of cardiac cells from ischemia-reperfusion injury. Human cardiac myocytes, endothelial cells, and cardiac fibroblast underwent ischemia-reperfusion conditions in the presence or in the absence of low frequency electromagnetic field. LDH and MMP-2 activities (as markers of cell injury), and cell metabolic activity (by fluorescein diacetate staining) were measured to determine the protective role of low frequency electromagnetic field. Our data showed that short courses of low frequency electromagnetic field protect cardiac cells from cellular damage and preserve their metabolic activity during ischemia-reperfusion. This study demonstrates the possibility to use of low frequency electromagnetic field as strategy for the prevention or therapy of ischemia-reperfusion injury. Impact statement In our study, we showed that LF-EMF may be protective for heart during ischemia-reperfusion (I/R). Following is the short description of the main findings: (a) the response to the I/R injury was different for endothelial cells, fibroblasts, and cardiomyocytes; (b) I/R decreases MMP-2 activity in cardiac myocytes and fibroblasts;

Machine Enhanced Logic

AI evidence extraction

Structured fields below were generated from the paper’s abstract and metadata to make the evidence easier to inspect. Automated extraction may be incomplete or incorrect; verify important details against the original paper.

Study typein_vitro

Effect directionbenefit

PopulationHuman cardiac myocytes, endothelial cells, and cardiac fibroblasts (cell culture)

Sample sizeNot supplied

ExposureELF · short courses (duration not specified)

Evidence strengthLowConfidence: 74% · Peer reviewed: yes

Main findings

Under ischemia-reperfusion conditions, short courses of low frequency electromagnetic field exposure were associated with reduced cellular damage and preserved metabolic activity in human cardiac cells. The abstract notes that responses differed across endothelial cells, fibroblasts, and cardiomyocytes, and that ischemia-reperfusion decreased MMP-2 activity in cardiac myocytes and fibroblasts.

Outcomes measured

Ischemia-reperfusion cell injury (LDH activity)
MMP-2 activity
Cell metabolic activity (fluorescein diacetate staining)
Reactive oxygen species production (mentioned as inhibited; measurement not described)

Limitations

No exposure parameters reported (frequency, field strength, waveform, exposure setup).
No sample size or replication details provided in the abstract.
Results are from in vitro human cells; clinical relevance is not established in the abstract.
Details on ROS measurement are not provided (only stated as inhibited).

View raw extracted JSON

{
  "study_type": "in_vitro",
  "exposure": {
    "band": "ELF",
    "source": null,
    "frequency_mhz": null,
    "sar_wkg": null,
    "duration": "short courses (duration not specified)"
  },
  "population": "Human cardiac myocytes, endothelial cells, and cardiac fibroblasts (cell culture)",
  "sample_size": null,
  "outcomes": [
    "Ischemia-reperfusion cell injury (LDH activity)",
    "MMP-2 activity",
    "Cell metabolic activity (fluorescein diacetate staining)",
    "Reactive oxygen species production (mentioned as inhibited; measurement not described)"
  ],
  "main_findings": "Under ischemia-reperfusion conditions, short courses of low frequency electromagnetic field exposure were associated with reduced cellular damage and preserved metabolic activity in human cardiac cells. The abstract notes that responses differed across endothelial cells, fibroblasts, and cardiomyocytes, and that ischemia-reperfusion decreased MMP-2 activity in cardiac myocytes and fibroblasts.",
  "effect_direction": "benefit",
  "limitations": [
    "No exposure parameters reported (frequency, field strength, waveform, exposure setup).",
    "No sample size or replication details provided in the abstract.",
    "Results are from in vitro human cells; clinical relevance is not established in the abstract.",
    "Details on ROS measurement are not provided (only stated as inhibited)."
  ],
  "evidence_strength": "low",
  "confidence": 0.74,
  "peer_reviewed_likely": "yes",
  "keywords": [
    "extremely low frequency",
    "low frequency electromagnetic field",
    "ELF-EMF",
    "ischemia-reperfusion",
    "cardiomyocytes",
    "endothelial cells",
    "cardiac fibroblasts",
    "LDH",
    "MMP-2",
    "metabolic activity",
    "oxidative stress"
  ],
  "suggested_hubs": []
}

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AI-extracted fields are generated from the abstract and metadata and may be incomplete or incorrect. This material is for informational purposes and is not medical advice.

Identifiers and source

DOI: 10.1177/1535370218779773
PubMed: 29848052

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