Effects of low frequency electromagnetic field on proliferation of human epidermal stem cells: An in vitro study.

Bioelectromagnetics · 2013 · Benefit · Low evidence

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BenefitLow evidencePaper 4504

Study snapshot

PublicationBioelectromagnetics · 2013

Study typein_vitro

Reported directionBenefit

Evidence ratingLow

Abstract

To investigate the effects of low frequency electromagnetic fields (EMF) on the proliferation of epidermal stem cells, human epidermal stem cells (hESC) were isolated, expanded ex vivo, and then exposed to a low frequency EMF. The test and control cells were placed under the same environment. The test cells were exposed for 30 min/day to a 5 mT low frequency EMF at 1, 10, and 50 Hz for 3, 5, or 7 days. The effects of low frequency EMF on cell proliferation, cell cycle, and cell-surface antigen phenotype were investigated. Low frequency EMF significantly enhanced the proliferation of hESC in the culture medium in a frequency-dependent manner, with the highest cell proliferation rate at 50 Hz (P < 0.05). Exposure to a low frequency EMF significantly increased the percentage of cells at the S phase of the cell cycle, coupled with a decrease in the percentage of cells in the G1 phase (P < 0.05) but the effect was not frequency dependent. The percentage of CD29(+) /CD71(-) cells remained unchanged in the low frequency EMF-exposed hESC. The results suggested that low frequency EMF influenced hESC proliferation in vitro, and this effect was related to the increased proportion of cells at the S phase.

Machine Enhanced Logic

AI evidence extraction

Structured fields below were generated from the paper’s abstract and metadata to make the evidence easier to inspect. Automated extraction may be incomplete or incorrect; verify important details against the original paper.

Study typein_vitro

Effect directionbenefit

PopulationHuman epidermal stem cells (hESC) in culture

Sample sizeNot supplied

ExposureELF · 30 min/day for 3, 5, or 7 days; 1, 10, or 50 Hz; 5 mT

Evidence strengthLowConfidence: 78% · Peer reviewed: yes

Main findings

Exposure to a 5 mT low frequency EMF (1, 10, 50 Hz) for 30 min/day over 3–7 days significantly enhanced proliferation of human epidermal stem cells in a frequency-dependent manner, with the highest proliferation at 50 Hz (P<0.05). EMF exposure increased the percentage of cells in S phase and decreased the percentage in G1 phase (P<0.05), while the CD29(+)/CD71(-) phenotype percentage remained unchanged.

Outcomes measured

Cell proliferation
Cell cycle phase distribution (S phase, G1 phase)
Cell-surface antigen phenotype (CD29(+)/CD71(-))

Limitations

In vitro study (cell culture), which may not generalize to in vivo human health outcomes
Sample size not reported in the abstract
Exposure source/setup details beyond field strength and frequency not described in the abstract

View raw extracted JSON

{
  "study_type": "in_vitro",
  "exposure": {
    "band": "ELF",
    "source": null,
    "frequency_mhz": null,
    "sar_wkg": null,
    "duration": "30 min/day for 3, 5, or 7 days; 1, 10, or 50 Hz; 5 mT"
  },
  "population": "Human epidermal stem cells (hESC) in culture",
  "sample_size": null,
  "outcomes": [
    "Cell proliferation",
    "Cell cycle phase distribution (S phase, G1 phase)",
    "Cell-surface antigen phenotype (CD29(+)/CD71(-))"
  ],
  "main_findings": "Exposure to a 5 mT low frequency EMF (1, 10, 50 Hz) for 30 min/day over 3–7 days significantly enhanced proliferation of human epidermal stem cells in a frequency-dependent manner, with the highest proliferation at 50 Hz (P<0.05). EMF exposure increased the percentage of cells in S phase and decreased the percentage in G1 phase (P<0.05), while the CD29(+)/CD71(-) phenotype percentage remained unchanged.",
  "effect_direction": "benefit",
  "limitations": [
    "In vitro study (cell culture), which may not generalize to in vivo human health outcomes",
    "Sample size not reported in the abstract",
    "Exposure source/setup details beyond field strength and frequency not described in the abstract"
  ],
  "evidence_strength": "low",
  "confidence": 0.78,
  "peer_reviewed_likely": "yes",
  "keywords": [
    "extremely low frequency",
    "ELF EMF",
    "low frequency electromagnetic field",
    "human epidermal stem cells",
    "proliferation",
    "cell cycle",
    "S phase",
    "G1 phase",
    "CD29",
    "CD71",
    "in vitro",
    "5 mT",
    "1 Hz",
    "10 Hz",
    "50 Hz"
  ],
  "suggested_hubs": []
}

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AI-extracted fields are generated from the abstract and metadata and may be incomplete or incorrect. This material is for informational purposes and is not medical advice.

Identifiers and source

DOI: 10.1002/bem.21747
PubMed: 22926783

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