Synergistic effect of rice bran extract and extremely low-frequency electromagnetic fields on dermal papilla/melanocytes in melanogenesis.

Bioelectromagnetics · 2018 · Benefit · Low evidence

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BenefitLow evidencePaper 5512

Study snapshot

PublicationBioelectromagnetics · 2018

Study typein_vitro

Reported directionBenefit

Evidence ratingLow

Abstract

Melanocytes in hair are located around dermal papilla cells at the tip of the hair follicle. In this study, we examined the melanogenesis of a three-dimensional (3D) hair dermal papilla model treated with natural extracts and electromagnetic fields (EMFs). The 3D model involved dermal papilla-like tissue (DPLT), an aggregation of a mixture of dermal papilla cells, and melanocytes in microwells. Rice bran extract (RBE), an EMF, and RBE/EMF were applied to different DPLT groups. The LDH assay indicated no cell stress in all experimental groups, and detection of tyrosinase activity demonstrated high activity in the RBE/EMF group. Western blot analysis of the RBE, EMF, and RBE/EMF groups revealed increased MITF, TRP-1, and tyrosinase expression. In addition, the mRNA expression of ET-1, laminin, bFGF, β-catenin, MITF, and tyrosinase was increased in the RBE/EMF group, as demonstrated by RT-qPCR analysis. HMB45 and Fontana-Masson immunostaining showed that the RBE/EMF group had the highest melanin content. Therefore, RBE and EMF may be used as a material and therapy, respectively, for the treatment of vitiligo and white hair, through activation of melanogenesis in melanocytes. Bioelectromagnetics. 39:595-603, 2018. © 2018 The Authors. Bioelectromagnetics Published by Wiley Periodicals, Inc..

Machine Enhanced Logic

AI evidence extraction

Structured fields below were generated from the paper’s abstract and metadata to make the evidence easier to inspect. Automated extraction may be incomplete or incorrect; verify important details against the original paper.

Study typein_vitro

Effect directionbenefit

PopulationNot supplied

Sample sizeNot supplied

ExposureELF

Evidence strengthLowConfidence: 74% · Peer reviewed: yes

Main findings

In a 3D dermal papilla-like tissue model containing dermal papilla cells and melanocytes, the combined rice bran extract plus extremely low-frequency EMF (RBE/EMF) group showed higher tyrosinase activity, increased melanogenesis-related protein and mRNA expression, and the highest melanin content compared with other groups. LDH assay indicated no cell stress in any experimental group.

Outcomes measured

Cell stress (LDH assay)
Tyrosinase activity
Protein expression (MITF, TRP-1, tyrosinase) by Western blot
mRNA expression (ET-1, laminin, bFGF, β-catenin, MITF, tyrosinase) by RT-qPCR
Melanin content (HMB45 and Fontana-Masson immunostaining)

Limitations

No EMF exposure parameters (e.g., frequency, field strength, duration) reported in the abstract
In vitro 3D model; clinical relevance to vitiligo/white hair not established in the abstract
Sample size and replication details not provided in the abstract

View raw extracted JSON

{
  "study_type": "in_vitro",
  "exposure": {
    "band": "ELF",
    "source": null,
    "frequency_mhz": null,
    "sar_wkg": null,
    "duration": null
  },
  "population": null,
  "sample_size": null,
  "outcomes": [
    "Cell stress (LDH assay)",
    "Tyrosinase activity",
    "Protein expression (MITF, TRP-1, tyrosinase) by Western blot",
    "mRNA expression (ET-1, laminin, bFGF, β-catenin, MITF, tyrosinase) by RT-qPCR",
    "Melanin content (HMB45 and Fontana-Masson immunostaining)"
  ],
  "main_findings": "In a 3D dermal papilla-like tissue model containing dermal papilla cells and melanocytes, the combined rice bran extract plus extremely low-frequency EMF (RBE/EMF) group showed higher tyrosinase activity, increased melanogenesis-related protein and mRNA expression, and the highest melanin content compared with other groups. LDH assay indicated no cell stress in any experimental group.",
  "effect_direction": "benefit",
  "limitations": [
    "No EMF exposure parameters (e.g., frequency, field strength, duration) reported in the abstract",
    "In vitro 3D model; clinical relevance to vitiligo/white hair not established in the abstract",
    "Sample size and replication details not provided in the abstract"
  ],
  "evidence_strength": "low",
  "confidence": 0.74,
  "peer_reviewed_likely": "yes",
  "keywords": [
    "extremely low-frequency electromagnetic fields",
    "ELF-EMF",
    "rice bran extract",
    "melanogenesis",
    "melanocytes",
    "dermal papilla cells",
    "3D model",
    "tyrosinase",
    "MITF",
    "TRP-1",
    "vitiligo",
    "white hair"
  ],
  "suggested_hubs": []
}

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AI-extracted fields are generated from the abstract and metadata and may be incomplete or incorrect. This material is for informational purposes and is not medical advice.

Identifiers and source

DOI: 10.1002/bem.22151
PubMed: 30371954

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