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The activation of melanogenesis by p-CREB and MITF signaling with extremely low-frequency electromagnetic fields on B16F10 melanoma.

Life sciences · 2016 · Benefit · Very low evidence

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BenefitVery low evidencePaper 4831

In B16F10 melanoma cells, 60–75 Hz ELF-EMFs increased secreted and cellular melanin content and tyrosinase activity, with upregulation of tyrosinase and TRP-1 expression. ELF-EMFs were associated with increased MITF and p-CREB signaling and reduced ERK phosph…

Study snapshot

PublicationLife sciences · 2016
Study typein_vitro
Reported directionBenefit
Evidence ratingVery low

Abstract

Melanin in the skin determines the skin color, and decreased melanin causes many hypopigmentation disorders and increased damage to skin by ultraviolet B (UVB) light irradiation. Here, we stimulate melanogenesis in B16F10 melanoma cells by using specific frequencies of ELF-EMFs. In this study, we focus on the melanogenesis of EMF-ELFs and find that 60-75Hz ELF-EMFs upregulate melanin synthesis by stimulated expression of tyrosinase and TRP-1 through inhibition of phosphorylation ERK, activation of CREB, and MITF up-regulation in B16F10 melanoma cells. The results show that 60-75Hz ELF-EMFs significantly increase secreted melanin, cellular melanin content, and tyrosinase activity, and the cell mitochondria activity, cell viability, and cell membrane condition are unchanged. Furthermore, the protein expression level of MITF and p-CREB signaling pathway are significantly increased. Moreover, 60Hz ELF-EMFs reduce the phosphorylate of ERK in B16F10 melanoma cells. These findings indicate that stimulation of melanogenesis by using ELF-EMFs has therapeutic potential for treating hypopigmentation disorders such as vitiligo.

Machine Enhanced Logic

AI evidence extraction

Structured fields below were generated from the paper’s abstract and metadata to make the evidence easier to inspect. Automated extraction may be incomplete or incorrect; verify important details against the original paper.

Study typein_vitro
Effect directionbenefit
PopulationB16F10 melanoma cells
Sample sizeNot supplied
ExposureELF
Evidence strengthVery lowConfidence: 78% · Peer reviewed: yes

Main findings

In B16F10 melanoma cells, 60–75 Hz ELF-EMFs increased secreted and cellular melanin content and tyrosinase activity, with upregulation of tyrosinase and TRP-1 expression. ELF-EMFs were associated with increased MITF and p-CREB signaling and reduced ERK phosphorylation, while mitochondrial activity, cell viability, and cell membrane condition were reported as unchanged.

Outcomes measured

  • Melanin synthesis (secreted melanin, cellular melanin content)
  • Tyrosinase activity
  • Tyrosinase and TRP-1 expression
  • MITF protein expression
  • p-CREB signaling
  • ERK phosphorylation
  • Mitochondrial activity
  • Cell viability
  • Cell membrane condition

Limitations

  • In vitro study in a melanoma cell line (B16F10), limiting direct inference to human skin or clinical outcomes
  • Exposure parameters beyond frequency (e.g., field strength, duration) are not provided in the abstract
  • No sample size or replication details are reported in the abstract
View raw extracted JSON
{
  "study_type": "in_vitro",
  "exposure": {
    "band": "ELF",
    "source": null,
    "frequency_mhz": null,
    "sar_wkg": null,
    "duration": null
  },
  "population": "B16F10 melanoma cells",
  "sample_size": null,
  "outcomes": [
    "Melanin synthesis (secreted melanin, cellular melanin content)",
    "Tyrosinase activity",
    "Tyrosinase and TRP-1 expression",
    "MITF protein expression",
    "p-CREB signaling",
    "ERK phosphorylation",
    "Mitochondrial activity",
    "Cell viability",
    "Cell membrane condition"
  ],
  "main_findings": "In B16F10 melanoma cells, 60–75 Hz ELF-EMFs increased secreted and cellular melanin content and tyrosinase activity, with upregulation of tyrosinase and TRP-1 expression. ELF-EMFs were associated with increased MITF and p-CREB signaling and reduced ERK phosphorylation, while mitochondrial activity, cell viability, and cell membrane condition were reported as unchanged.",
  "effect_direction": "benefit",
  "limitations": [
    "In vitro study in a melanoma cell line (B16F10), limiting direct inference to human skin or clinical outcomes",
    "Exposure parameters beyond frequency (e.g., field strength, duration) are not provided in the abstract",
    "No sample size or replication details are reported in the abstract"
  ],
  "evidence_strength": "very_low",
  "confidence": 0.78,
  "peer_reviewed_likely": "yes",
  "keywords": [
    "extremely low-frequency electromagnetic fields",
    "ELF-EMF",
    "60 Hz",
    "75 Hz",
    "melanogenesis",
    "melanin",
    "B16F10",
    "tyrosinase",
    "TRP-1",
    "MITF",
    "CREB",
    "p-CREB",
    "ERK phosphorylation",
    "vitiligo",
    "hypopigmentation"
  ],
  "suggested_hubs": []
}
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AI-extracted fields are generated from the abstract and metadata and may be incomplete or incorrect. This material is for informational purposes and is not medical advice.

Identifiers and source

DOI: 10.1016/j.lfs.2016.08.015
PubMed: 27543340

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