Electromagnetic field-induced stimulation of Bruton's tyrosine kinase.

The Journal of biological chemistry · 1998 · Mixed · Low evidence

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MixedLow evidencePaper 5388

Study snapshot

PublicationThe Journal of biological chemistry · 1998

Study typein_vitro

Reported directionMixed

Evidence ratingLow

Abstract

Here we present evidence that exposure of DT40 lymphoma B-cells to low energy electromagnetic fields (EMF) results in activation of phospholipase C-gamma 2 (PLC-gamma2), leading to increased inositol phospholipid turnover. PLC-gamma2 activation in EMF-stimulated cells is mediated by stimulation of the Bruton's tyrosine kinase (BTK), a member of the Src-related TEC family of protein tyrosine kinases, which acts downstream of LYN kinase and upstream of PLC-gamma2. B-cells rendered BTK-deficient by targeted disruption of the btk gene did not show enhanced PLC-gamma2 activation in response to EMF exposure. Introduction of the wild-type (but not a kinase domain mutant) human btk gene into BTK-deficient B-cells restored their EMF responsiveness. Thus, BTK exerts a pivotal and mandatory function in initiation of EMF-induced signaling cascades in B-cells.

Machine Enhanced Logic

AI evidence extraction

Structured fields below were generated from the paper’s abstract and metadata to make the evidence easier to inspect. Automated extraction may be incomplete or incorrect; verify important details against the original paper.

Study typein_vitro

Effect directionmixed

PopulationDT40 lymphoma B-cells (chicken B-cell line)

Sample sizeNot supplied

ExposureNot supplied

Evidence strengthLowConfidence: 74% · Peer reviewed: yes

Main findings

Exposure of DT40 lymphoma B-cells to low energy electromagnetic fields was associated with activation of PLC-gamma2 and increased inositol phospholipid turnover. EMF-induced PLC-gamma2 activation depended on BTK: BTK-deficient cells did not show enhanced PLC-gamma2 activation with EMF exposure, and reintroduction of wild-type (but not kinase-domain mutant) human btk restored EMF responsiveness.

Outcomes measured

Bruton's tyrosine kinase (BTK) stimulation/requirement
Phospholipase C-gamma2 (PLC-gamma2) activation
Inositol phospholipid turnover

Limitations

No EMF exposure parameters reported in the abstract (e.g., frequency, field strength, duration, SAR).
In vitro cell-line model; generalizability to in vivo health outcomes is not addressed in the abstract.
Sample size and statistical details are not provided in the abstract.

View raw extracted JSON

{
  "study_type": "in_vitro",
  "exposure": {
    "band": null,
    "source": null,
    "frequency_mhz": null,
    "sar_wkg": null,
    "duration": null
  },
  "population": "DT40 lymphoma B-cells (chicken B-cell line)",
  "sample_size": null,
  "outcomes": [
    "Bruton's tyrosine kinase (BTK) stimulation/requirement",
    "Phospholipase C-gamma2 (PLC-gamma2) activation",
    "Inositol phospholipid turnover"
  ],
  "main_findings": "Exposure of DT40 lymphoma B-cells to low energy electromagnetic fields was associated with activation of PLC-gamma2 and increased inositol phospholipid turnover. EMF-induced PLC-gamma2 activation depended on BTK: BTK-deficient cells did not show enhanced PLC-gamma2 activation with EMF exposure, and reintroduction of wild-type (but not kinase-domain mutant) human btk restored EMF responsiveness.",
  "effect_direction": "mixed",
  "limitations": [
    "No EMF exposure parameters reported in the abstract (e.g., frequency, field strength, duration, SAR).",
    "In vitro cell-line model; generalizability to in vivo health outcomes is not addressed in the abstract.",
    "Sample size and statistical details are not provided in the abstract."
  ],
  "evidence_strength": "low",
  "confidence": 0.74,
  "peer_reviewed_likely": "yes",
  "keywords": [
    "electromagnetic fields",
    "EMF",
    "DT40",
    "B-cells",
    "lymphoma",
    "Bruton's tyrosine kinase",
    "BTK",
    "LYN kinase",
    "TEC family",
    "protein tyrosine kinase",
    "phospholipase C-gamma2",
    "PLC-gamma2",
    "inositol phospholipid turnover",
    "signal transduction"
  ],
  "suggested_hubs": []
}

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AI-extracted fields are generated from the abstract and metadata and may be incomplete or incorrect. This material is for informational purposes and is not medical advice.

Identifiers and source

DOI: 10.1074/jbc.273.20.12397
PubMed: 9575194

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