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Bruton's tyrosine kinase activity and inositol 1,4,5-trisphosphate production are not altered in DT40 lymphoma B cells exposed to power line frequency magnetic fields.

The Journal of biological chemistry · 1998 · No effect · Low evidence

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No effectLow evidencePaper 5377

In blinded exposure experiments with anti-IgM as a positive control, a 1-gauss 60-Hz EMF had no significant effect on Ins 1,4,5-P3 production. Using cells from the original investigators, the authors found no evidence supporting a causal effect of 1-gauss 60-…

Study snapshot

PublicationThe Journal of biological chemistry · 1998
Study typein_vitro
Reported directionNo effect
Evidence ratingLow

Abstract

Exposure of wild-type DT40 lymphoma B cells or Bruton's tyrosine kinase (BTK)-deficient DT40 cells reconstituted with the human btk gene to a 1-gauss 60-Hz electromagnetic field (EMF) has been reported to rapidly increase inositol 1,4,5-trisphosphate (Ins 1,4, 5-P3) production (1,2). Here we have used BTK-deficient DT40 B cells reconstituted with the human btk gene to evaluate the reproducibility of these findings. An experimental design with blinded exposures and anti-IgM treatment to induce Ins 1,4,5-P3 production as a positive control, showed no significant effect of a 1-gauss 60-Hz EMF on Ins 1,4,5-P3 production. Because recent work has shown that the activation of BTK was required for EMF-responsiveness (2), we also evaluated the reproducibility of this finding in wild-type DT40 cells. BTK was activated in a dose- and time-dependent manner by treatment with the tyrosine phosphatase inhibitor pervanadate. However, the ability to detect BTK activation, as measured by increased autophosphorylation by immune complex kinase assay, was dependent on the kinase buffer. Using cells from the original investigators, no evidence was obtained to support the hypothesis that exposure to a 1-gauss 60-Hz EMF had a causal effect on protein-tyrosine kinase activities affecting Ins 1,4,5-P3 production.

Machine Enhanced Logic

AI evidence extraction

Structured fields below were generated from the paper’s abstract and metadata to make the evidence easier to inspect. Automated extraction may be incomplete or incorrect; verify important details against the original paper.

Study typein_vitro
Effect directionno_effect
PopulationDT40 lymphoma B cells (wild-type and BTK-deficient DT40 cells reconstituted with human btk gene)
Sample sizeNot supplied
ExposureELF · 6e-05 MHz
Evidence strengthLowConfidence: 78% · Peer reviewed: yes

Main findings

In blinded exposure experiments with anti-IgM as a positive control, a 1-gauss 60-Hz EMF had no significant effect on Ins 1,4,5-P3 production. Using cells from the original investigators, the authors found no evidence supporting a causal effect of 1-gauss 60-Hz EMF exposure on protein-tyrosine kinase activities affecting Ins 1,4,5-P3 production.

Outcomes measured

  • Inositol 1,4,5-trisphosphate (Ins 1,4,5-P3) production
  • Bruton's tyrosine kinase (BTK) activation/autophosphorylation
  • Protein-tyrosine kinase activities affecting Ins 1,4,5-P3 production

Limitations

  • Exposure duration not reported in abstract
  • Sample size not reported in abstract
  • BTK activation detection depended on kinase buffer conditions, indicating methodological sensitivity
View raw extracted JSON
{
  "study_type": "in_vitro",
  "exposure": {
    "band": "ELF",
    "source": "power line frequency magnetic fields",
    "frequency_mhz": 6e-05,
    "sar_wkg": null,
    "duration": null
  },
  "population": "DT40 lymphoma B cells (wild-type and BTK-deficient DT40 cells reconstituted with human btk gene)",
  "sample_size": null,
  "outcomes": [
    "Inositol 1,4,5-trisphosphate (Ins 1,4,5-P3) production",
    "Bruton's tyrosine kinase (BTK) activation/autophosphorylation",
    "Protein-tyrosine kinase activities affecting Ins 1,4,5-P3 production"
  ],
  "main_findings": "In blinded exposure experiments with anti-IgM as a positive control, a 1-gauss 60-Hz EMF had no significant effect on Ins 1,4,5-P3 production. Using cells from the original investigators, the authors found no evidence supporting a causal effect of 1-gauss 60-Hz EMF exposure on protein-tyrosine kinase activities affecting Ins 1,4,5-P3 production.",
  "effect_direction": "no_effect",
  "limitations": [
    "Exposure duration not reported in abstract",
    "Sample size not reported in abstract",
    "BTK activation detection depended on kinase buffer conditions, indicating methodological sensitivity"
  ],
  "evidence_strength": "low",
  "confidence": 0.78,
  "peer_reviewed_likely": "yes",
  "keywords": [
    "ELF EMF",
    "60-Hz",
    "1 gauss",
    "DT40",
    "lymphoma B cells",
    "Bruton's tyrosine kinase",
    "BTK",
    "inositol 1,4,5-trisphosphate",
    "InsP3",
    "protein-tyrosine kinase",
    "blinded exposure",
    "reproducibility"
  ],
  "suggested_hubs": [
    {
      "slug": "occupational-exposure",
      "weight": 0.2,
      "reason": "Study concerns power line frequency (60-Hz) magnetic field exposure, though conducted in vitro rather than in workers."
    }
  ]
}
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AI-extracted fields are generated from the abstract and metadata and may be incomplete or incorrect. This material is for informational purposes and is not medical advice.

Identifiers and source

DOI: 10.1074/jbc.273.49.32618
PubMed: 9830001

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