Hatching in zebrafish (Danio rerio) embryos exposed to a 50 Hz magnetic field.

Bioelectromagnetics · 2000 · Mixed · Low evidence

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MixedLow evidencePaper 5344

Zebrafish embryos exposed intra ovo to a 50 Hz AC magnetic field (1000 microT rms) showed a statistically significant delay in hatching when exposure began 48 hours after fertilization. When exposure began 2 hours after fertilization, no statistically signifi…

Study snapshot

PublicationBioelectromagnetics · 2000
Study typeanimal
Reported directionMixed
Evidence ratingLow

Abstract

Zebrafish embryos were exposed intra ovo to a 50 Hz AC magnetic field of 1000 microT rms, and the progress of asynchronous hatching was monitored. A statistically significant delay was observed when field exposure started 48 h after fertilization. In contrast, when exposure started 2 h after fertilization, no statistically significant effect was seen. When field exposure was administered together with submaximal doses of progesterone at 48 h postfertilization, the two treatments appeared to delay hatching in an additive manner. Evaluating the progress of hatching in zebrafish embryos seems relevant for exploration of EMF effects on reproduction.

Machine Enhanced Logic

AI evidence extraction

Structured fields below were generated from the paper’s abstract and metadata to make the evidence easier to inspect. Automated extraction may be incomplete or incorrect; verify important details against the original paper.

Study typeanimal
Effect directionmixed
PopulationZebrafish (Danio rerio) embryos (intra ovo)
Sample sizeNot supplied
ExposureELF · 0.05 MHz
Evidence strengthLowConfidence: 74% · Peer reviewed: yes

Main findings

Zebrafish embryos exposed intra ovo to a 50 Hz AC magnetic field (1000 microT rms) showed a statistically significant delay in hatching when exposure began 48 hours after fertilization. When exposure began 2 hours after fertilization, no statistically significant effect on hatching was observed. Co-exposure with submaximal progesterone doses at 48 hours postfertilization appeared to delay hatching additively.

Outcomes measured

  • Hatching timing/progress (asynchronous hatching delay)

Limitations

  • Sample size not reported in abstract
  • Exposure duration and other exposure parameters beyond frequency and field strength not reported in abstract
  • Only hatching progress assessed; broader reproductive/developmental endpoints not described in abstract
View raw extracted JSON
{
  "study_type": "animal",
  "exposure": {
    "band": "ELF",
    "source": null,
    "frequency_mhz": 0.05,
    "sar_wkg": null,
    "duration": null
  },
  "population": "Zebrafish (Danio rerio) embryos (intra ovo)",
  "sample_size": null,
  "outcomes": [
    "Hatching timing/progress (asynchronous hatching delay)"
  ],
  "main_findings": "Zebrafish embryos exposed intra ovo to a 50 Hz AC magnetic field (1000 microT rms) showed a statistically significant delay in hatching when exposure began 48 hours after fertilization. When exposure began 2 hours after fertilization, no statistically significant effect on hatching was observed. Co-exposure with submaximal progesterone doses at 48 hours postfertilization appeared to delay hatching additively.",
  "effect_direction": "mixed",
  "limitations": [
    "Sample size not reported in abstract",
    "Exposure duration and other exposure parameters beyond frequency and field strength not reported in abstract",
    "Only hatching progress assessed; broader reproductive/developmental endpoints not described in abstract"
  ],
  "evidence_strength": "low",
  "confidence": 0.74,
  "peer_reviewed_likely": "yes",
  "keywords": [
    "zebrafish",
    "Danio rerio",
    "embryo",
    "hatching",
    "50 Hz",
    "AC magnetic field",
    "ELF",
    "1000 microT",
    "progesterone",
    "development"
  ],
  "suggested_hubs": [
    {
      "slug": "elf-emf",
      "weight": 0.9,
      "reason": "Animal experiment with 50 Hz magnetic field exposure."
    },
    {
      "slug": "reproduction-development",
      "weight": 0.6,
      "reason": "Outcome relates to embryonic development/hatching."
    }
  ]
}
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AI-extracted fields are generated from the abstract and metadata and may be incomplete or incorrect. This material is for informational purposes and is not medical advice.

Identifiers and source

DOI: 10.1002/1521-186x(200007)21:5<407::aid-bem10>3.0.co;2-v
PubMed: 10899777

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