Measurement of 100 MHz EMF radiation in vivo effects on zebrafish embryonic development

2018 · Mixed · Low evidence

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MixedLow evidencePaper 2102

Study snapshot

Publicationmanual · 2018

Study typeanimal

Reported directionMixed

Evidence ratingLow

Abstract

Measurement of 100 MHz EMF radiation in vivo effects on zebrafish embryonic development Piccinetti CC, De Leo A, Cosoli G, Scalise L, Randazzo B, Cerri B. Olivotto I. Measurement of the 100 MHz EMF radiation in vivo effects on zebrafish D. rerio embryonic development: A multidisciplinary study. Ecotoxicology and Environmental Safety. 154:268-279. June 2018. doi.org Highlights • Setting up of an EMF exposure protocol for the unequivocal interpretation of results. • Assessment of biological effects arising from RF-EMF exposure during zebrafish embryonic development. • Zebrafish as a useful experimental model in electromagnetic pollution studies. Abstract The augmented exposure of both environment and human being to electromagnetic waves and the concomitant lack of an unequivocal knowledge about biological consequences of these radiations, raised public interest on electromagnetic pollution. In this context, the present study aims to evaluate the biological effects on zebrafish (ZF) embryos of 100 MHz radiofrequency electromagnetic field (RF-EMF) exposure through a multidisciplinary protocol. Because of the shared synteny between human and ZF genomes that validated its use in biomedical research, toxicology and developmental biology studies, ZF was here selected as experimental model and a measurement protocol and biological analyses have been set up to clearly discriminate between RF-EMF biological and thermal effects. The results showed that a 100 MHz EMF was able to affect ZF embryonic development, from 24 to 72 h post fertilization (hpf) in all the analyzed pathways. Particularly, at the 48 hpf stage, a reduced growth, an increased transcription of oxidative stress genes, the onset of apoptotic/autophagic processes and a modification in cholesterol metabolism were detected. ZF embryos faced stress induced by EMF radiation by triggering detoxification mechanisms and at 72 hpf they partially recovered from stress reaching the hatching time in a comparable way respect to the control group. Data here obtained showed unequivocally the in vivo effects of RF-EMF on an animal model, excluding thermal outcomes and thus represents the starting point for more comprehensive studies on dose response effects of electromagnetic fields radiations consequences. sciencedirect.com

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AI evidence extraction

Structured fields below were generated from the paper’s abstract and metadata to make the evidence easier to inspect. Automated extraction may be incomplete or incorrect; verify important details against the original paper.

Study typeanimal

Effect directionmixed

PopulationZebrafish (Danio rerio) embryos

Sample sizeNot supplied

ExposureRF · 100 MHz · 24 to 72 h post fertilization (hpf)

Evidence strengthLowConfidence: 74% · Peer reviewed: yes

Main findings

In zebrafish embryos exposed to a 100 MHz RF-EMF from 24 to 72 hpf, the authors report effects on embryonic development across analyzed pathways. At 48 hpf they observed reduced growth, increased transcription of oxidative stress genes, onset of apoptotic/autophagic processes, and modified cholesterol metabolism; by 72 hpf embryos partially recovered and reached hatching in a comparable way to controls, with thermal effects stated to be excluded.

Outcomes measured

Embryonic development (growth)
Oxidative stress gene transcription
Apoptotic/autophagic processes
Cholesterol metabolism
Detoxification mechanisms/stress response
Hatching (at 72 hpf)

Limitations

Sample size not reported in provided abstract/metadata
Exposure metrics such as SAR or field strength not reported in provided abstract/metadata
Details of exposure protocol and control conditions not provided in provided abstract/metadata
Outcome measurement methods and statistical results not provided in provided abstract/metadata

View raw extracted JSON

{
  "study_type": "animal",
  "exposure": {
    "band": "RF",
    "source": null,
    "frequency_mhz": 100,
    "sar_wkg": null,
    "duration": "24 to 72 h post fertilization (hpf)"
  },
  "population": "Zebrafish (Danio rerio) embryos",
  "sample_size": null,
  "outcomes": [
    "Embryonic development (growth)",
    "Oxidative stress gene transcription",
    "Apoptotic/autophagic processes",
    "Cholesterol metabolism",
    "Detoxification mechanisms/stress response",
    "Hatching (at 72 hpf)"
  ],
  "main_findings": "In zebrafish embryos exposed to a 100 MHz RF-EMF from 24 to 72 hpf, the authors report effects on embryonic development across analyzed pathways. At 48 hpf they observed reduced growth, increased transcription of oxidative stress genes, onset of apoptotic/autophagic processes, and modified cholesterol metabolism; by 72 hpf embryos partially recovered and reached hatching in a comparable way to controls, with thermal effects stated to be excluded.",
  "effect_direction": "mixed",
  "limitations": [
    "Sample size not reported in provided abstract/metadata",
    "Exposure metrics such as SAR or field strength not reported in provided abstract/metadata",
    "Details of exposure protocol and control conditions not provided in provided abstract/metadata",
    "Outcome measurement methods and statistical results not provided in provided abstract/metadata"
  ],
  "evidence_strength": "low",
  "confidence": 0.74,
  "peer_reviewed_likely": "yes",
  "keywords": [
    "100 MHz",
    "RF-EMF",
    "radiofrequency",
    "zebrafish",
    "Danio rerio",
    "embryonic development",
    "oxidative stress",
    "apoptosis",
    "autophagy",
    "cholesterol metabolism",
    "thermal effects excluded"
  ],
  "suggested_hubs": []
}

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AI-extracted fields are generated from the abstract and metadata and may be incomplete or incorrect. This material is for informational purposes and is not medical advice.

Identifiers and source

DOI: 10.1016/j.ecoenv.2018.02.053
PubMed: Not supplied

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