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Lyn and syk tyrosine kinases are not activated in B-lineage lymphoid cells exposed to low-energy electromagnetic fields.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2000 · No effect · Low evidence

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No effectLow evidencePaper 5337

In blinded, carefully controlled experiments, exposure to a 100 microT 60 Hz AC magnetic field (with and without a 46 microT DC magnetic field) produced no significant effects on Lyn or Syk tyrosine kinase activities or on protein tyrosine phosphorylation in …

Study snapshot

PublicationFASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2000
Study typein_vitro
Reported directionNo effect
Evidence ratingLow

Abstract

Exposure of B-lineage lymphoid cells to a 100 microT 60 Hz AC magnetic field has been reported to stimulate the rapid activation of Lyn and Syk tyrosine kinases and the induction of protein tyrosine phosphorylation. These findings are significant because of the critical role played by these B cell signaling events in the control of growth and differentiation, and therefore the potential of electromagnetic field (EMF) exposure to induce cancer. We report the first study carried out with the aim of reproducing the reported EMF effects on Lyn and Syk tyrosine kinases. The system used enabled EMF exposure conditions to be carefully controlled and also allowed experiments to be performed blind. The effects of a 100 microT 60 Hz AC magnetic field on protein tyrosine phosphorylation and on Lyn and Syk tyrosine kinase activities were investigated in Nalm-6 and DT40 B cells in the absence and presence of a 46 microT DC magnetic field. However, no significant effects of low-energy electromagnetic fields on tyrosine kinase activities or protein phosphorylation were observed.

Machine Enhanced Logic

AI evidence extraction

Structured fields below were generated from the paper’s abstract and metadata to make the evidence easier to inspect. Automated extraction may be incomplete or incorrect; verify important details against the original paper.

Study typein_vitro
Effect directionno_effect
PopulationNalm-6 and DT40 B-lineage lymphoid (B) cells
Sample sizeNot supplied
ExposureELF · 0.06 MHz
Evidence strengthLowConfidence: 74% · Peer reviewed: yes

Main findings

In blinded, carefully controlled experiments, exposure to a 100 microT 60 Hz AC magnetic field (with and without a 46 microT DC magnetic field) produced no significant effects on Lyn or Syk tyrosine kinase activities or on protein tyrosine phosphorylation in Nalm-6 and DT40 B cells.

Outcomes measured

  • Lyn tyrosine kinase activity
  • Syk tyrosine kinase activity
  • Protein tyrosine phosphorylation

Limitations

  • Sample size not reported in abstract
  • Exposure duration not reported in abstract
  • Only two B-cell lines studied
View raw extracted JSON
{
  "study_type": "in_vitro",
  "exposure": {
    "band": "ELF",
    "source": null,
    "frequency_mhz": 0.06,
    "sar_wkg": null,
    "duration": null
  },
  "population": "Nalm-6 and DT40 B-lineage lymphoid (B) cells",
  "sample_size": null,
  "outcomes": [
    "Lyn tyrosine kinase activity",
    "Syk tyrosine kinase activity",
    "Protein tyrosine phosphorylation"
  ],
  "main_findings": "In blinded, carefully controlled experiments, exposure to a 100 microT 60 Hz AC magnetic field (with and without a 46 microT DC magnetic field) produced no significant effects on Lyn or Syk tyrosine kinase activities or on protein tyrosine phosphorylation in Nalm-6 and DT40 B cells.",
  "effect_direction": "no_effect",
  "limitations": [
    "Sample size not reported in abstract",
    "Exposure duration not reported in abstract",
    "Only two B-cell lines studied"
  ],
  "evidence_strength": "low",
  "confidence": 0.74,
  "peer_reviewed_likely": "yes",
  "keywords": [
    "ELF magnetic field",
    "60 Hz",
    "100 microT",
    "DC magnetic field",
    "46 microT",
    "B-lineage lymphoid cells",
    "Nalm-6",
    "DT40",
    "Lyn",
    "Syk",
    "tyrosine phosphorylation",
    "blinded experiment",
    "reproducibility"
  ],
  "suggested_hubs": [
    {
      "slug": "occupational-exposure",
      "weight": 0.25,
      "reason": "Study involves 60 Hz ELF magnetic-field exposure relevant to power-frequency environments, though no specific occupational setting is stated."
    }
  ]
}
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AI-extracted fields are generated from the abstract and metadata and may be incomplete or incorrect. This material is for informational purposes and is not medical advice.

Identifiers and source

DOI: 10.1096/fj.00-0164com
PubMed: 11053250

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