Extended exposure of adult and fetal mice to 50 Hz magnetic field does not increase the incidence of micronuclei in erythrocytes.

Bioelectromagnetics · 2001 · No effect · Low evidence

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No effectLow evidencePaper 5319

Study snapshot

PublicationBioelectromagnetics · 2001

Study typeanimal

Reported directionNo effect

Evidence ratingLow

Abstract

The flow cytometer-based micronucleus assay was used to study the effects on chromosomes in erythroid cells of CBA/Ca mice after extended exposure to 50 Hz magnetic field (MF), 14 microT, peak-to-peak (p-p). The study included two different experiments: (a) mice exposed in utero during 18 days of their prenatal stage, and (b) adult mice exposed for 18 days. In experiment (a) 35 days after exposure was terminated, peripheral blood was drawn from the mice exposed in utero to determine whether the exposure had a genotoxic effect on the pluripotent erythroid stem cells. About 200000 polychromatic erythrocytes (PCE) and 200000 normochromatic erythrocytes (NCE) were analysed from each of 20 exposed mice. The EMF exposure did not significantly change the frequency of micronucleated PCE or NCE in comparison with 20 sham-irradiated mice. There was no difference in the proportion of PCE between exposed and unexposed animals. Similarly, in experiment (b) no differences were seen between EMF exposed and unexposed adult mice when samples of peripheral blood were taken at the end of exposure and analyzed for micronuclei in PCE and NCE. The proportion of PCE was the same in both groups. The results indicate that exposure to EMF does not induce direct or indirect effects on chromosomes in erythroid cells expressed as increased levels of micronucleated erythrocytes of mice. No indications of delayed genetic effects were found.

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AI evidence extraction

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Study typeanimal

Effect directionno_effect

PopulationCBA/Ca mice (adult and in utero exposed fetuses assessed postnatally)

Sample size40

ExposureELF · 0.05 MHz · 18 days (in utero prenatal exposure for 18 days; adult exposure for 18 days)

Evidence strengthLowConfidence: 86% · Peer reviewed: yes

Main findings

CBA/Ca mice exposed to a 50 Hz magnetic field (14 microT peak-to-peak) for 18 days in utero showed no significant differences in micronucleated PCE or NCE compared with sham-irradiated controls when assessed 35 days after exposure ended. Adult mice exposed for 18 days also showed no differences in micronuclei in PCE or NCE at the end of exposure, and PCE proportions were similar between exposed and unexposed groups.

Outcomes measured

Micronuclei frequency in polychromatic erythrocytes (PCE)
Micronuclei frequency in normochromatic erythrocytes (NCE)
Proportion of PCE

View raw extracted JSON

{
  "study_type": "animal",
  "exposure": {
    "band": "ELF",
    "source": null,
    "frequency_mhz": 0.05,
    "sar_wkg": null,
    "duration": "18 days (in utero prenatal exposure for 18 days; adult exposure for 18 days)"
  },
  "population": "CBA/Ca mice (adult and in utero exposed fetuses assessed postnatally)",
  "sample_size": 40,
  "outcomes": [
    "Micronuclei frequency in polychromatic erythrocytes (PCE)",
    "Micronuclei frequency in normochromatic erythrocytes (NCE)",
    "Proportion of PCE"
  ],
  "main_findings": "CBA/Ca mice exposed to a 50 Hz magnetic field (14 microT peak-to-peak) for 18 days in utero showed no significant differences in micronucleated PCE or NCE compared with sham-irradiated controls when assessed 35 days after exposure ended. Adult mice exposed for 18 days also showed no differences in micronuclei in PCE or NCE at the end of exposure, and PCE proportions were similar between exposed and unexposed groups.",
  "effect_direction": "no_effect",
  "limitations": [],
  "evidence_strength": "low",
  "confidence": 0.86,
  "peer_reviewed_likely": "yes",
  "keywords": [
    "50 Hz",
    "ELF magnetic field",
    "14 microT",
    "mouse",
    "CBA/Ca",
    "in utero exposure",
    "adult exposure",
    "micronucleus assay",
    "flow cytometry",
    "genotoxicity",
    "erythrocytes",
    "chromosomal damage"
  ],
  "suggested_hubs": []
}

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AI-extracted fields are generated from the abstract and metadata and may be incomplete or incorrect. This material is for informational purposes and is not medical advice.

Identifiers and source

DOI: 10.1002/bem.61
PubMed: 11424159

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