Effect of electromagnetic fields on proliferation and differentiation of cultured mouse bone marrow mesenchymal stem cells.

Journal of Huazhong University of Science and Technology. Medical sciences = Hua zhong ke ji da xue xue bao. Yi xue Ying De wen ban = Huazhong keji daxue xuebao. Yixue Yingdewen ban · 2005 · Mixed · Low evidence

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MixedLow evidencePaper 5166

Exposure to EMF (50 Hz pulse burst, 2 mT peak) inhibited MSC proliferation (P<0.05), enhanced differentiation as measured by ALP activity (P<0.05), and increased intracellular cAMP levels early in stimulation (1–3 days; P<0.01). cAMP did not increase further …

Study snapshot

PublicationJournal of Huazhong University of Science and Technology. Medical sciences = Hua zhong ke ji da xue xue bao. Yi xue Ying De wen ban = Huazhong keji daxue xuebao. Yixue Yingdewen ban · 2005

Study typein_vitro

Reported directionMixed

Evidence ratingLow

Abstract

In order to study the effects of electromagnetic fields (EMFs) on proliferation, differentiation and intercellular cyclic AMP (cAMP) in mouse bone marrow mesenchymal stem cells (MSCs) in vitro, the mouse bone MSCs were isolated and cultured in vitro. The third passage MSCs were divided into 4 groups and stimulated with EMFs. The cellular proliferation (MTT), the cellular differentiation (alkaline phosphatase activity, ALP), and the intercellular cAMP level were investigated at different time points. The results showed that EMF (50Hz pulse burst 2 mT peak) inhibited the cellular proliferation (P < 0.05), enhanced the cellular differentiation (P < 0. 05), and increased the intercellular cAMP level (P < 0.01) in the early time of the stimulation (1-3 days), but the intercellular cAMP level did not increased further in the later days. We are led to conclude that the cAMP may be involved in the mediation of the growth inhibitory and differentiation-inducing signals of specific EMFs in vitro.

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AI evidence extraction

Structured fields below were generated from the paper’s abstract and metadata to make the evidence easier to inspect. Automated extraction may be incomplete or incorrect; verify important details against the original paper.

Study typein_vitro

Effect directionmixed

PopulationCultured mouse bone marrow mesenchymal stem cells (MSCs), third passage

Sample sizeNot supplied

ExposureELF · 1–3 days (early time points); later days also assessed (duration not specified)

Evidence strengthLowConfidence: 78% · Peer reviewed: yes

Main findings

Outcomes measured

Cell proliferation (MTT)
Cell differentiation (alkaline phosphatase activity, ALP)
Intracellular cyclic AMP (cAMP) level

Limitations

In vitro study (mouse MSC culture)
Sample size not reported in abstract
Exposure duration and later time points not fully specified in abstract
Only specific endpoints reported (MTT, ALP, cAMP)

View raw extracted JSON

{
  "study_type": "in_vitro",
  "exposure": {
    "band": "ELF",
    "source": null,
    "frequency_mhz": null,
    "sar_wkg": null,
    "duration": "1–3 days (early time points); later days also assessed (duration not specified)"
  },
  "population": "Cultured mouse bone marrow mesenchymal stem cells (MSCs), third passage",
  "sample_size": null,
  "outcomes": [
    "Cell proliferation (MTT)",
    "Cell differentiation (alkaline phosphatase activity, ALP)",
    "Intracellular cyclic AMP (cAMP) level"
  ],
  "main_findings": "Exposure to EMF (50 Hz pulse burst, 2 mT peak) inhibited MSC proliferation (P<0.05), enhanced differentiation as measured by ALP activity (P<0.05), and increased intracellular cAMP levels early in stimulation (1–3 days; P<0.01). cAMP did not increase further at later time points.",
  "effect_direction": "mixed",
  "limitations": [
    "In vitro study (mouse MSC culture)",
    "Sample size not reported in abstract",
    "Exposure duration and later time points not fully specified in abstract",
    "Only specific endpoints reported (MTT, ALP, cAMP)"
  ],
  "evidence_strength": "low",
  "confidence": 0.78,
  "peer_reviewed_likely": "yes",
  "keywords": [
    "electromagnetic fields",
    "ELF-EMF",
    "50 Hz",
    "2 mT",
    "pulse burst",
    "mesenchymal stem cells",
    "mouse",
    "proliferation",
    "differentiation",
    "alkaline phosphatase",
    "cAMP",
    "in vitro"
  ],
  "suggested_hubs": []
}

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AI-extracted fields are generated from the abstract and metadata and may be incomplete or incorrect. This material is for informational purposes and is not medical advice.

Identifiers and source

DOI: 10.1007/bf02873572
PubMed: 16116968

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