Changes in intracellular calcium concentration level accompany age-related inhibitions of Long-term potentiation in hippocampus induced by Extremely low frequency electromagnetic fields

The European journal of neuroscience · 2023 · Harm · Low evidence

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HarmLow evidencePaper 483

Study snapshot

PublicationThe European journal of neuroscience · 2023

Study typeanimal

Reported directionHarm

Evidence ratingLow

Abstract

Changes in intracellular calcium concentration level accompany age-related inhibitions of Long-term potentiation in hippocampus induced by Extremely low frequency electromagnetic fields Zhao W, Dong L, Tian L, Zhao L, Zhao Y, Zheng Y. Changes in intracellular calcium concentration level accompany age-related inhibitions of Long-term potentiation in hippocampus induced by Extremely low frequency electromagnetic fields. Eur J Neurosci. 2023 May 18. doi: 10.1111/ejn.16046. Abstract Extremely low frequency electromagnetic fields (ELF-EMFs) have received increasing attention and in-depth research due to their ability to affect learning and memory functions. However, its regulation and intrinsic mechanism at different ages in early developmental stages remains unclear. In this article, the regulation of 15 Hz/2 mT ELF-EMFs on Long-term potentiation (LTP) persistence in the hippocampal CA1 region of Sprague- Dawley (SD) rats at early developmental stages (8, 15, 22, and 29-day-old) are investigated by electrophysiological techniques. The results shows that ELF-EMFs differentially inhibit LTP persistence due to age difference, and the younger age, the more significant the inhibitory effect. Secondly, the inhibitory effect of ELF-EMFs on LTP persistence disappeared after the addition of 2-Aminoethoxydiphenyl borate (2-APB) to block inositol-1,4,5-trisphosphate receptors (IP3 Rs) localized to intracellular calcium stores to reduce the intracellular calcium concentration ([Ca2+ ]i ), proving that the LTP persistence regulated by ELF-EMFs is associated with the IP3 Rs-mediated intracellular calcium stores. Finally, the level of [Ca2+ ]i was intervened by adjusting the extracellular calcium concentration([Ca2+ ]e ). Interestingly, the inhibitory effect of ELF-EMFs on LTP persistence in the 15-day-old group disappeared by increasing [Ca2+ ]e , while the inhibitory effect of ELF-EMFs on LTP persistence in the 29-day-old group appeared by decreasing [Ca2+ ]e . Our findings reveal the underlying mechanism of the effect of ELF-EMFs on synaptic plasticity in hippocampal CA1 area at early developmental stages, and provide new insights into more rational application and protection of ELF-EMFs. pubmed.ncbi.nlm.nih.gov

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AI evidence extraction

Structured fields below were generated from the paper’s abstract and metadata to make the evidence easier to inspect. Automated extraction may be incomplete or incorrect; verify important details against the original paper.

Study typeanimal

Effect directionharm

PopulationSprague-Dawley rats at early developmental stages (8-, 15-, 22-, and 29-day-old)

Sample sizeNot supplied

ExposureELF · 1.5e-05 MHz

Evidence strengthLowConfidence: 78% · Peer reviewed: yes

Main findings

In electrophysiological experiments in rat hippocampal CA1, 15 Hz/2 mT ELF-EMF exposure inhibited LTP persistence in an age-dependent manner, with stronger inhibition at younger ages. The inhibitory effect disappeared when IP3 receptors were blocked with 2-APB to reduce intracellular calcium, and manipulating extracellular calcium altered whether inhibition was present (increasing [Ca2+]e removed inhibition in 15-day-old rats; decreasing [Ca2+]e induced inhibition in 29-day-old rats).

Outcomes measured

Hippocampal CA1 long-term potentiation (LTP) persistence
Intracellular calcium concentration ([Ca2+]i)
Effects of IP3 receptor blockade (2-APB) on ELF-EMF-related LTP changes
Effects of extracellular calcium concentration ([Ca2+]e) manipulation on ELF-EMF-related LTP changes

Limitations

Exposure duration not reported in the abstract
Sample size not reported in the abstract
Animal study; generalizability to humans not addressed in the abstract
Outcomes are electrophysiological/mechanistic (LTP, calcium) rather than behavioral learning/memory measures in the abstract

View raw extracted JSON

{
  "study_type": "animal",
  "exposure": {
    "band": "ELF",
    "source": null,
    "frequency_mhz": 1.5e-05,
    "sar_wkg": null,
    "duration": null
  },
  "population": "Sprague-Dawley rats at early developmental stages (8-, 15-, 22-, and 29-day-old)",
  "sample_size": null,
  "outcomes": [
    "Hippocampal CA1 long-term potentiation (LTP) persistence",
    "Intracellular calcium concentration ([Ca2+]i)",
    "Effects of IP3 receptor blockade (2-APB) on ELF-EMF-related LTP changes",
    "Effects of extracellular calcium concentration ([Ca2+]e) manipulation on ELF-EMF-related LTP changes"
  ],
  "main_findings": "In electrophysiological experiments in rat hippocampal CA1, 15 Hz/2 mT ELF-EMF exposure inhibited LTP persistence in an age-dependent manner, with stronger inhibition at younger ages. The inhibitory effect disappeared when IP3 receptors were blocked with 2-APB to reduce intracellular calcium, and manipulating extracellular calcium altered whether inhibition was present (increasing [Ca2+]e removed inhibition in 15-day-old rats; decreasing [Ca2+]e induced inhibition in 29-day-old rats).",
  "effect_direction": "harm",
  "limitations": [
    "Exposure duration not reported in the abstract",
    "Sample size not reported in the abstract",
    "Animal study; generalizability to humans not addressed in the abstract",
    "Outcomes are electrophysiological/mechanistic (LTP, calcium) rather than behavioral learning/memory measures in the abstract"
  ],
  "evidence_strength": "low",
  "confidence": 0.78,
  "peer_reviewed_likely": "yes",
  "keywords": [
    "extremely low frequency electromagnetic fields",
    "ELF-EMF",
    "15 Hz",
    "2 mT",
    "hippocampus",
    "CA1",
    "long-term potentiation",
    "LTP persistence",
    "intracellular calcium",
    "IP3 receptor",
    "2-APB",
    "developmental stage",
    "Sprague-Dawley rat"
  ],
  "suggested_hubs": [
    {
      "slug": "elf-emf",
      "weight": 0.9,
      "reason": "Study investigates extremely low frequency EMF exposure (15 Hz, 2 mT) and neurophysiological effects."
    },
    {
      "slug": "mechanisms-calcium-signaling",
      "weight": 0.75,
      "reason": "Findings focus on intracellular calcium/IP3 receptor-mediated mechanisms underlying EMF effects on synaptic plasticity."
    },
    {
      "slug": "neurodevelopment",
      "weight": 0.6,
      "reason": "Age-dependent effects examined across early developmental stages (8–29 days old)."
    }
  ]
}

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AI-extracted fields are generated from the abstract and metadata and may be incomplete or incorrect. This material is for informational purposes and is not medical advice.

Identifiers and source

DOI: 10.1111/ejn.16046
PubMed: 37203290

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