[Global gene response to GSM 1800 MHz radiofrequency electromagnetic field in MCF-7 cells].

Zhonghua yu fang yi xue za zhi [Chinese journal of preventive medicine] · 2006 · Unclear · Very low evidence

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UnclearVery low evidencePaper 5118

Study snapshot

PublicationZhonghua yu fang yi xue za zhi [Chinese journal of preventive medicine] · 2006

Study typein_vitro

Reported directionUnclear

Evidence ratingVery low

Abstract

OBJECTIVE: To investigate whether GSM 1800 MHz radiofrequency electromagnetic field (RF EMF) can change the gene expression profile in MCF-7 cells and to screen RF EMF responsive genes. METHODS: Subcultured MCF-7 cells were intermittently (5-minute fields on/10-minute fields off) exposed or sham-exposed to GSM 1800 MHz RF EMF, which was modulated by 217 Hz EMF, for 24 hours at an average specific absorption rate (SAR) of 2.0 W/kg or 3.5 W/kg. Immediately after RF EMF exposure or sham-exposure, total RNA was isolated from MCF-7 cells and then purified. Affymetrix Human Genome U133A Genechip was applied to examine the change of gene expression profile according to the manufacturer's instruction. Data was analyzed by Affymetrix Microarray Suite 5.0 (MAS 5.0) and Affymetrix Data Mining Tool 3.0 (DMT 3.0). Quantitative reverse transcription polymerase chain reaction (RT-PCR) was used to validate the differentially expressed genes identified by Genechip analysis. RESULTS: A small number of differential expression genes were found in each comparison after RF EMF exposure. Through reproducible and consistent analysis, no gene or five up-regulated genes were screened out after exposure to RF EMF at SAR of 2.0 W/kg or 3.5 W/kg, respectively. However, these five genes could not be further confirmed by RT-PCR. CONCLUSION: The present study did not provide clear evidence that RF EMF exposure might distinctly change the gene expression profile in MCF-7 cells under current experimental conditions, implying that the exposure might not affect the MCF-7 cell physiology, or this cell line might be less sensitive to the RF EMF exposure.

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AI evidence extraction

Structured fields below were generated from the paper’s abstract and metadata to make the evidence easier to inspect. Automated extraction may be incomplete or incorrect; verify important details against the original paper.

Study typein_vitro

Effect directionunclear

PopulationMCF-7 cells

Sample sizeNot supplied

ExposureRF · 1800 MHz · 24 hours (intermittent: 5 min on/10 min off)

Evidence strengthVery lowConfidence: 78% · Peer reviewed: yes

Main findings

After intermittent GSM 1800 MHz RF-EMF exposure for 24 hours, only a small number of differentially expressed genes were observed; no reproducible genes were identified at 2.0 W/kg, and five up-regulated genes were screened at 3.5 W/kg by microarray analysis. These five genes were not confirmed by RT-PCR, and the authors concluded there was no clear evidence of distinct gene expression changes under the experimental conditions.

Outcomes measured

Global gene expression profile (Affymetrix Human Genome U133A microarray)
Differentially expressed genes
RT-PCR validation of candidate genes

Limitations

In vitro cell line model (MCF-7) may not generalize to in vivo effects
Candidate gene changes detected by microarray were not confirmed by RT-PCR
Sample size/number of replicates not reported in the abstract

View raw extracted JSON

{
  "study_type": "in_vitro",
  "exposure": {
    "band": "RF",
    "source": null,
    "frequency_mhz": 1800,
    "sar_wkg": null,
    "duration": "24 hours (intermittent: 5 min on/10 min off)"
  },
  "population": "MCF-7 cells",
  "sample_size": null,
  "outcomes": [
    "Global gene expression profile (Affymetrix Human Genome U133A microarray)",
    "Differentially expressed genes",
    "RT-PCR validation of candidate genes"
  ],
  "main_findings": "After intermittent GSM 1800 MHz RF-EMF exposure for 24 hours, only a small number of differentially expressed genes were observed; no reproducible genes were identified at 2.0 W/kg, and five up-regulated genes were screened at 3.5 W/kg by microarray analysis. These five genes were not confirmed by RT-PCR, and the authors concluded there was no clear evidence of distinct gene expression changes under the experimental conditions.",
  "effect_direction": "unclear",
  "limitations": [
    "In vitro cell line model (MCF-7) may not generalize to in vivo effects",
    "Candidate gene changes detected by microarray were not confirmed by RT-PCR",
    "Sample size/number of replicates not reported in the abstract"
  ],
  "evidence_strength": "very_low",
  "confidence": 0.78,
  "peer_reviewed_likely": "yes",
  "keywords": [
    "GSM",
    "1800 MHz",
    "RF-EMF",
    "MCF-7",
    "gene expression",
    "microarray",
    "Affymetrix U133A",
    "RT-PCR",
    "SAR",
    "217 Hz modulation"
  ],
  "suggested_hubs": []
}

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AI-extracted fields are generated from the abstract and metadata and may be incomplete or incorrect. This material is for informational purposes and is not medical advice.

Identifiers and source

DOI: Not supplied
PubMed: 16836876

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