[Effects of GSM 1800 MHz radiofrequency electromagnetic fields on protein expression profile of human breast cancer cell MCF-7].

Zhonghua yu fang yi xue za zhi [Chinese journal of preventive medicine] · 2006 · Mixed · Low evidence

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MixedLow evidencePaper 5119

Study snapshot

PublicationZhonghua yu fang yi xue za zhi [Chinese journal of preventive medicine] · 2006

Study typein_vitro

Reported directionMixed

Evidence ratingLow

Abstract

OBJECTIVE: To study the effects of GSM 1800 MHz radiofrequency electromagnetic fields (RF EMF) exposure on protein expression profile of human breast cancer cell line (MCF-7), as to exploring the possible effects on normal cell physiological function. METHODS: MCF-7 cells were continuously or intermittently (5 minutes field on followed by 10 minutes off) exposed to RF EMF for different duration (1 hour, 3 hours, 6 hours, 12 hours, or 24 hours) at an average specific absorption rate (SAR) of 3.5 W/kg. The extracted proteins were separated by 2-dimensional electrophoresis and the protein-spot distribution of the silver-stained gels was analyzed by using PDQuest software 7.1. Each experiment was repeated three times. RESULTS: On the average, around 1100 proteins were detected using pH 4 - 7 IPG strip. There were no differential proteins found under continuous exposure at SAR of 3.5 W/kg for 6 hours. Under other exposure conditions, we found various differentially expressed proteins in exposure groups as compared with the sham-exposed controls. Especially in 3 hours intermittent exposure and 12 hours continuous exposure, eighteen and seven differential proteins were detected, respectively. The categories and functions of these differentially expressed proteins were analyzed by searching of SWISS-PROT protein database, which suggested that these proteins should be related to the functions of biosynthesization, signal transduction, and DNA damage and repair. CONCLUSIONS: Data indicated that the protein expression changes induced by RF radiation might depend on exposure duration and mode. Many biological processes might be affected by RF exposure.

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AI evidence extraction

Structured fields below were generated from the paper’s abstract and metadata to make the evidence easier to inspect. Automated extraction may be incomplete or incorrect; verify important details against the original paper.

Study typein_vitro

Effect directionmixed

PopulationHuman breast cancer cell line MCF-7

Sample sizeNot supplied

ExposureRF · 1800 MHz · 3.5 W/kg · 1, 3, 6, 12, or 24 hours; continuous or intermittent (5 min on/10 min off)

Evidence strengthLowConfidence: 78% · Peer reviewed: yes

Main findings

No differential proteins were found after 6 hours of continuous exposure at SAR 3.5 W/kg. Under other exposure conditions, various differentially expressed proteins were observed versus sham controls; 18 differential proteins were detected after 3 hours intermittent exposure and 7 after 12 hours continuous exposure. Database searching suggested these proteins were related to biosynthesis, signal transduction, and DNA damage/repair functions.

Outcomes measured

Protein expression profile (2D electrophoresis; differential protein spots)

Limitations

In vitro study in a cancer cell line (MCF-7), not normal cells
Sample size/number of independent biological replicates beyond 'each experiment repeated three times' not fully specified
Differential proteins are described by spot counts and database category suggestions; specific protein identities and validation methods are not provided in the abstract

View raw extracted JSON

{
  "study_type": "in_vitro",
  "exposure": {
    "band": "RF",
    "source": "GSM mobile phone signal (GSM 1800)",
    "frequency_mhz": 1800,
    "sar_wkg": 3.5,
    "duration": "1, 3, 6, 12, or 24 hours; continuous or intermittent (5 min on/10 min off)"
  },
  "population": "Human breast cancer cell line MCF-7",
  "sample_size": null,
  "outcomes": [
    "Protein expression profile (2D electrophoresis; differential protein spots)"
  ],
  "main_findings": "No differential proteins were found after 6 hours of continuous exposure at SAR 3.5 W/kg. Under other exposure conditions, various differentially expressed proteins were observed versus sham controls; 18 differential proteins were detected after 3 hours intermittent exposure and 7 after 12 hours continuous exposure. Database searching suggested these proteins were related to biosynthesis, signal transduction, and DNA damage/repair functions.",
  "effect_direction": "mixed",
  "limitations": [
    "In vitro study in a cancer cell line (MCF-7), not normal cells",
    "Sample size/number of independent biological replicates beyond 'each experiment repeated three times' not fully specified",
    "Differential proteins are described by spot counts and database category suggestions; specific protein identities and validation methods are not provided in the abstract"
  ],
  "evidence_strength": "low",
  "confidence": 0.78,
  "peer_reviewed_likely": "yes",
  "keywords": [
    "GSM",
    "1800 MHz",
    "radiofrequency electromagnetic fields",
    "RF EMF",
    "specific absorption rate",
    "SAR 3.5 W/kg",
    "MCF-7",
    "breast cancer cells",
    "proteomics",
    "2-dimensional electrophoresis",
    "intermittent exposure",
    "continuous exposure"
  ],
  "suggested_hubs": []
}

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AI-extracted fields are generated from the abstract and metadata and may be incomplete or incorrect. This material is for informational purposes and is not medical advice.

Identifiers and source

DOI: Not supplied
PubMed: 16836875

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