Effects of sinusoidal electromagnetic field on structure and function of different kinds of cell lines.

Yonsei medical journal · 2006 · Mixed · Low evidence

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MixedLow evidencePaper 5094

Study snapshot

PublicationYonsei medical journal · 2006

Study typein_vitro

Reported directionMixed

Evidence ratingLow

Abstract

This study investigated that whether a 2 mT, 60 Hz, sinusoidal electromagnetic field (EMF) alters the structure and function of cells. This research compared the effects of EMF on four kinds of cell lines: hFOB 1.19 (fetal osteoblast), T/G HA-VSMC (aortic vascular smooth muscle cell), RPMI 7666 (B lymphoblast), and HCN-2 (cortical neuronal cell). Over 14 days, cells were exposed to EMF for 1, 3, or 6 hours per day (hrs/d). The results pointed to a cell type-specific reaction to EMF exposure. In addition, the cellular responses were dependent on duration of EMF exposure. In the present study, cell proliferation was the trait most sensitive to EMF. EMF treatment promoted growth of hFOB 1.19 and HCN-2 compared with control cells at 7 and 14 days of incubation. When the exposure time was 3 hrs/d, EMF enhanced the proliferation of RPMI 7666 but inhibited that of T/G HA- VSMC. On the other hand, the effects of EMF on cell cycle distribution, cell differentiation, and actin distribution were unclear. Furthermore, we hardly found any correlation between EMF exposure and gap junctional intercellular communication in hFOB 1.19. This study revealed that EMF might serve as a potential tool for manipulating cell proliferation.

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AI evidence extraction

Structured fields below were generated from the paper’s abstract and metadata to make the evidence easier to inspect. Automated extraction may be incomplete or incorrect; verify important details against the original paper.

Study typein_vitro

Effect directionmixed

PopulationCell lines: hFOB 1.19 (fetal osteoblast), T/G HA-VSMC (aortic vascular smooth muscle cell), RPMI 7666 (B lymphoblast), HCN-2 (cortical neuronal cell)

Sample sizeNot supplied

ExposureELF · 0.06 MHz · 14 days; 1, 3, or 6 hours/day

Evidence strengthLowConfidence: 78% · Peer reviewed: yes

Main findings

A 2 mT, 60 Hz sinusoidal EMF produced cell type- and exposure-duration-dependent effects. Proliferation was most sensitive: growth was promoted in hFOB 1.19 and HCN-2 at 7 and 14 days; at 3 hours/day, proliferation increased in RPMI 7666 but was inhibited in T/G HA-VSMC. Effects on cell cycle distribution, differentiation, and actin distribution were unclear, and little correlation was found with gap junctional intercellular communication in hFOB 1.19.

Outcomes measured

Cell structure and function (general)
Cell proliferation/growth
Cell cycle distribution
Cell differentiation
Actin distribution
Gap junctional intercellular communication

Limitations

Sample size not reported in abstract
In vitro cell-line study; generalizability to humans not addressed in abstract
Several endpoints reported as unclear (cell cycle distribution, differentiation, actin distribution)

View raw extracted JSON

{
  "study_type": "in_vitro",
  "exposure": {
    "band": "ELF",
    "source": null,
    "frequency_mhz": 0.06,
    "sar_wkg": null,
    "duration": "14 days; 1, 3, or 6 hours/day"
  },
  "population": "Cell lines: hFOB 1.19 (fetal osteoblast), T/G HA-VSMC (aortic vascular smooth muscle cell), RPMI 7666 (B lymphoblast), HCN-2 (cortical neuronal cell)",
  "sample_size": null,
  "outcomes": [
    "Cell structure and function (general)",
    "Cell proliferation/growth",
    "Cell cycle distribution",
    "Cell differentiation",
    "Actin distribution",
    "Gap junctional intercellular communication"
  ],
  "main_findings": "A 2 mT, 60 Hz sinusoidal EMF produced cell type- and exposure-duration-dependent effects. Proliferation was most sensitive: growth was promoted in hFOB 1.19 and HCN-2 at 7 and 14 days; at 3 hours/day, proliferation increased in RPMI 7666 but was inhibited in T/G HA-VSMC. Effects on cell cycle distribution, differentiation, and actin distribution were unclear, and little correlation was found with gap junctional intercellular communication in hFOB 1.19.",
  "effect_direction": "mixed",
  "limitations": [
    "Sample size not reported in abstract",
    "In vitro cell-line study; generalizability to humans not addressed in abstract",
    "Several endpoints reported as unclear (cell cycle distribution, differentiation, actin distribution)"
  ],
  "evidence_strength": "low",
  "confidence": 0.78,
  "peer_reviewed_likely": "yes",
  "keywords": [
    "extremely low frequency",
    "ELF EMF",
    "60 Hz",
    "2 mT",
    "sinusoidal electromagnetic field",
    "cell lines",
    "cell proliferation",
    "cell cycle",
    "differentiation",
    "actin distribution",
    "gap junctional intercellular communication"
  ],
  "suggested_hubs": []
}

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AI-extracted fields are generated from the abstract and metadata and may be incomplete or incorrect. This material is for informational purposes and is not medical advice.

Identifiers and source

DOI: 10.3349/ymj.2006.47.6.852
PubMed: 17191316

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