Electromagnetic fields promote osteogenesis of rat mesenchymal stem cells through the PKA and ERK1/2 pathways.

Journal of tissue engineering and regenerative medicine · 2016 · Benefit · Low evidence

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BenefitLow evidencePaper 4696

EMF exposure promoted osteogenesis in rat bone marrow MSCs and increased expression of several osteogenic markers. EMF increased cAMP levels and was associated with PKA and ERK1/2 phosphorylation; inhibition of MEK1/2 (PD98059) or PKA (H-89) significantly inh…

Study snapshot

PublicationJournal of tissue engineering and regenerative medicine · 2016

Study typein_vitro

Reported directionBenefit

Evidence ratingLow

Abstract

It has been reported that electromagnetic fields (EMFs) can promote the healing of non-union, osteogenesis and differentiation of the osteoblasts. However, its mechanism has not been unravelled. In this study, we detected some response induced by EMF and evaluated the importance of these signals for EMF-induced osteogenesis in bone marrow mesenchymal stem cells (MSCs). We characterized the expression of EMF-induced osteogenesis markers in MSCs, using RT-PCR and real-time PCR. Western blot was used to detect the signalling pathways. We found that EMF could promote osteogenesis in MSCs, along with the expression of several osteogenic markers. EMF-induced cyclic adenosine monophosphate (cAMP) level increase causes protein kinase A (PKA) and extracellular signal-regulated kinase (ERK)1/2 phosphorylation. Pretreating the MSCs with the mitogen-activated protein kinase (MAPK)/ERK kinase 1/2 (MEK1/2) inhibitor PD98059, or the PKA inhibitor H-89, significantly inhibited the induction of osteogenic markers, showing that EMF induction of osteogenesis was dependent on the ERK and PKA signalling pathways. Therefore, our study showed that EMF promoted MSC osteogenesis and that the EMF-induced osteogenic markers were mediated by both the PKA and MAPK signalling pathways. Copyright © 2014 John Wiley & Sons, Ltd.

Machine Enhanced Logic

AI evidence extraction

Structured fields below were generated from the paper’s abstract and metadata to make the evidence easier to inspect. Automated extraction may be incomplete or incorrect; verify important details against the original paper.

Study typein_vitro

Effect directionbenefit

PopulationRat bone marrow mesenchymal stem cells (MSCs)

Sample sizeNot supplied

ExposureNot supplied

Evidence strengthLowConfidence: 74% · Peer reviewed: yes

Main findings

Outcomes measured

Osteogenesis in MSCs
Expression of osteogenic markers (RT-PCR/real-time PCR)
cAMP level changes
PKA phosphorylation/activation
ERK1/2 phosphorylation/activation
Effect of MEK1/2 inhibitor PD98059 on osteogenic marker induction
Effect of PKA inhibitor H-89 on osteogenic marker induction

Limitations

Exposure parameters (e.g., frequency, intensity, duration) are not reported in the abstract.
In vitro study in rat cells; generalizability to in vivo or human outcomes is not addressed in the abstract.
Sample size and quantitative effect sizes are not provided in the abstract.

View raw extracted JSON

{
  "study_type": "in_vitro",
  "exposure": {
    "band": null,
    "source": null,
    "frequency_mhz": null,
    "sar_wkg": null,
    "duration": null
  },
  "population": "Rat bone marrow mesenchymal stem cells (MSCs)",
  "sample_size": null,
  "outcomes": [
    "Osteogenesis in MSCs",
    "Expression of osteogenic markers (RT-PCR/real-time PCR)",
    "cAMP level changes",
    "PKA phosphorylation/activation",
    "ERK1/2 phosphorylation/activation",
    "Effect of MEK1/2 inhibitor PD98059 on osteogenic marker induction",
    "Effect of PKA inhibitor H-89 on osteogenic marker induction"
  ],
  "main_findings": "EMF exposure promoted osteogenesis in rat bone marrow MSCs and increased expression of several osteogenic markers. EMF increased cAMP levels and was associated with PKA and ERK1/2 phosphorylation; inhibition of MEK1/2 (PD98059) or PKA (H-89) significantly inhibited induction of osteogenic markers, suggesting dependence on ERK and PKA pathways.",
  "effect_direction": "benefit",
  "limitations": [
    "Exposure parameters (e.g., frequency, intensity, duration) are not reported in the abstract.",
    "In vitro study in rat cells; generalizability to in vivo or human outcomes is not addressed in the abstract.",
    "Sample size and quantitative effect sizes are not provided in the abstract."
  ],
  "evidence_strength": "low",
  "confidence": 0.74,
  "peer_reviewed_likely": "yes",
  "keywords": [
    "electromagnetic fields",
    "EMF",
    "osteogenesis",
    "mesenchymal stem cells",
    "rat",
    "cAMP",
    "PKA",
    "ERK1/2",
    "MAPK",
    "MEK inhibitor",
    "PD98059",
    "H-89",
    "osteogenic markers"
  ],
  "suggested_hubs": []
}

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AI-extracted fields are generated from the abstract and metadata and may be incomplete or incorrect. This material is for informational purposes and is not medical advice.

Identifiers and source

DOI: 10.1002/term.1864
PubMed: 24634418

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