Effect of exposure to an extremely low frequency-electromagnetic field on the cellular collagen with respect to signaling pathways in osteoblast-like cells.

The journal of medical investigation : JMI · 2008 · Benefit · Low evidence

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BenefitLow evidencePaper 4998

Exposure to ELF-EMF (3 mT, 60 Hz) significantly increased cellular collagen content in MC3T3-E1 cells. ERK1/2 inhibition (PD98059) reduced collagen overall but did not prevent the ELF-EMF-induced increase; PI3K inhibition (LY294002) accelerated the ELF-EMF-in…

Study snapshot

PublicationThe journal of medical investigation : JMI · 2008

Study typein_vitro

Reported directionBenefit

Evidence ratingLow

Abstract

The effect of exposure to extremely low frequency-electromagnetic field (ELF-EMF: 3 mT, 60 Hz) on differentiation of mouse osteoblast-like MC3T3-E1 cells was examined together with addition of insulin-like growth factor I (IGF-I). As a marker of the differentiation, the cellular collagen content was determined by the absorbance of Sirius red-stained cells measured at the wavelength of 510-520 nm with an imaging microspectroscopy. Exposure to ELF-EMF increased significantly the collagen in the cells. Treatment with PD98059, an inhibitor of extracellular signal-regulated kinase 1/2 (ERK1/2) activation, reduced the collagen in all of the cells examined on control, IGF-I addition and ELF-EMF exposure, however, PD98059 did not prevent the increase in the collagen caused by ELF-EMF exposure, and IGF-I also increased the collagen in the presence of the inhibitor. When phosphatidylinositol 3-kinase (PI3K) pathway was inhibited by LY294002, the increase in collagen induced by ELF-EMF exposure was accelerated, however, the increase in collagen observed by IGF-I addition was suppressed. Treatment with SB203580, an inhibitor of p38 mitogen-activated protein kinase (p38 MAPK), suppressed the increase in the collagen induced by ELF-EMF exposure, whereas IGF-I addition increased the collagen in the presence of the inhibitor. These results suggested that collagen synthesis stimulated by ELF-EMF exposure was carried out by the participation of p38 MAPK pathway, and that PI3K pathway may have the role to suppress the collagen synthesis induced by ELF-EMF exposure, and that the suppression of the PI3K pathway may allow the acceleration of the collagen synthesis.

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AI evidence extraction

Structured fields below were generated from the paper’s abstract and metadata to make the evidence easier to inspect. Automated extraction may be incomplete or incorrect; verify important details against the original paper.

Study typein_vitro

Effect directionbenefit

PopulationMouse osteoblast-like MC3T3-E1 cells

Sample sizeNot supplied

ExposureELF · 6e-05 MHz

Evidence strengthLowConfidence: 78% · Peer reviewed: yes

Main findings

Outcomes measured

Cellular collagen content (Sirius red staining absorbance 510–520 nm)
Osteoblast-like cell differentiation marker (collagen)
Signaling pathway involvement (ERK1/2, PI3K, p38 MAPK) via pharmacologic inhibitors

Limitations

In vitro cell model; findings may not generalize to organisms or humans
Exposure duration not reported in abstract
Sample size and replication details not reported in abstract
Outcome focused on collagen content as a differentiation marker; limited scope of biological endpoints

View raw extracted JSON

{
  "study_type": "in_vitro",
  "exposure": {
    "band": "ELF",
    "source": null,
    "frequency_mhz": 6e-05,
    "sar_wkg": null,
    "duration": null
  },
  "population": "Mouse osteoblast-like MC3T3-E1 cells",
  "sample_size": null,
  "outcomes": [
    "Cellular collagen content (Sirius red staining absorbance 510–520 nm)",
    "Osteoblast-like cell differentiation marker (collagen)",
    "Signaling pathway involvement (ERK1/2, PI3K, p38 MAPK) via pharmacologic inhibitors"
  ],
  "main_findings": "Exposure to ELF-EMF (3 mT, 60 Hz) significantly increased cellular collagen content in MC3T3-E1 cells. ERK1/2 inhibition (PD98059) reduced collagen overall but did not prevent the ELF-EMF-induced increase; PI3K inhibition (LY294002) accelerated the ELF-EMF-induced collagen increase; p38 MAPK inhibition (SB203580) suppressed the ELF-EMF-induced collagen increase.",
  "effect_direction": "benefit",
  "limitations": [
    "In vitro cell model; findings may not generalize to organisms or humans",
    "Exposure duration not reported in abstract",
    "Sample size and replication details not reported in abstract",
    "Outcome focused on collagen content as a differentiation marker; limited scope of biological endpoints"
  ],
  "evidence_strength": "low",
  "confidence": 0.78,
  "peer_reviewed_likely": "yes",
  "keywords": [
    "ELF-EMF",
    "extremely low frequency",
    "60 Hz",
    "3 mT",
    "osteoblast-like cells",
    "MC3T3-E1",
    "collagen",
    "differentiation",
    "IGF-I",
    "ERK1/2",
    "PI3K",
    "p38 MAPK",
    "PD98059",
    "LY294002",
    "SB203580"
  ],
  "suggested_hubs": []
}

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AI-extracted fields are generated from the abstract and metadata and may be incomplete or incorrect. This material is for informational purposes and is not medical advice.

Identifiers and source

DOI: 10.2152/jmi.55.267
PubMed: 18797142

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