Effects of extremely low frequency electromagnetic fields on paraoxonase serum activity and lipid peroxidation metabolites in rat

J Diabetes Metab Disord · 2014 · Harm · Low evidence

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HarmLow evidencePaper 2742

Study snapshot

PublicationJ Diabetes Metab Disord · 2014

Study typeanimal

Reported directionHarm

Evidence ratingLow

Abstract

Background: Atherogenic effects of ELF-MF exposure have not been studied well so far. Therefore we have hypothesized that ELF-MF exposure might have atherogenic effect by impairing antioxidant function and increasing lipid peroxidation. This study was therefore undertaken to examine the effects of ELF-MF on paraoxonase (PON) activity, antioxidant capacity and lipid peroxidation metabolites. Effects of time on remodeling of antioxidant system were also investigated in this study. Methods: Seventy five Wistar rats were randomly allocated into five groups as follows: 1) Sham exposure, 2) Single exposure to 60 Hz, sacrificed immediately after exposure, 3) Single exposure to 60 Hz, sacrificed 72 hours after exposure, 4) Fourteen days of exposure to 60 Hz, sacrificed immediately after exposure, and 5) Fourteen days of exposure to 60 Hz, sacrificed 72 hours after exposure. Blood samples were collected and analyzed. The results were compared using ANOVA and post hoc Tukey HSD for multiple caparisons. Results: Single ELF-MF exposure significantly increased lipid peroxidation (CD and MDA) and increased antioxidant serum activity (HDL, paraoxonase activity, and serum total antioxidant capacity). Chronic ELF-MF exposure increased lipid peroxidation and affected antioxidant system. Free fatty acids levels were significantly increased after both single and two weeks exposure. Chronic exposure led to irreversible changes while acute exposure tended to reversible alterations on above mentioned parameters. Conclusions: According to the results of this study, ELF-MF exposure could impair oxidant-antioxidant function and might increase oxidative stress and lipid peroxidation. Antioxidant capability was dependent on the duration and continuity of ELF-MF exposure.

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AI evidence extraction

Structured fields below were generated from the paper’s abstract and metadata to make the evidence easier to inspect. Automated extraction may be incomplete or incorrect; verify important details against the original paper.

Study typeanimal

Effect directionharm

PopulationWistar rats

Sample size75

ExposureELF · 6e-05 MHz · Single exposure (sacrificed immediately or 72h later) and 14 days exposure (sacrificed immediately or 72h later)

Evidence strengthLowConfidence: 78% · Peer reviewed: yes

Main findings

Single 60 Hz ELF-MF exposure significantly increased lipid peroxidation markers (CD, MDA) and increased antioxidant-related serum measures (HDL, paraoxonase activity, total antioxidant capacity). Chronic (14-day) exposure increased lipid peroxidation and affected the antioxidant system; free fatty acids increased after both single and two-week exposure. The authors report chronic exposure led to irreversible changes while acute exposure tended toward reversible alterations.

Outcomes measured

Paraoxonase (PON) activity
Serum total antioxidant capacity
HDL
Lipid peroxidation (conjugated dienes, malondialdehyde)
Free fatty acids

View raw extracted JSON

{
  "study_type": "animal",
  "exposure": {
    "band": "ELF",
    "source": null,
    "frequency_mhz": 6e-05,
    "sar_wkg": null,
    "duration": "Single exposure (sacrificed immediately or 72h later) and 14 days exposure (sacrificed immediately or 72h later)"
  },
  "population": "Wistar rats",
  "sample_size": 75,
  "outcomes": [
    "Paraoxonase (PON) activity",
    "Serum total antioxidant capacity",
    "HDL",
    "Lipid peroxidation (conjugated dienes, malondialdehyde)",
    "Free fatty acids"
  ],
  "main_findings": "Single 60 Hz ELF-MF exposure significantly increased lipid peroxidation markers (CD, MDA) and increased antioxidant-related serum measures (HDL, paraoxonase activity, total antioxidant capacity). Chronic (14-day) exposure increased lipid peroxidation and affected the antioxidant system; free fatty acids increased after both single and two-week exposure. The authors report chronic exposure led to irreversible changes while acute exposure tended toward reversible alterations.",
  "effect_direction": "harm",
  "limitations": [],
  "evidence_strength": "low",
  "confidence": 0.78,
  "peer_reviewed_likely": "yes",
  "keywords": [
    "extremely low frequency",
    "ELF-MF",
    "60 Hz",
    "oxidative stress",
    "lipid peroxidation",
    "malondialdehyde",
    "conjugated dienes",
    "paraoxonase",
    "antioxidant capacity",
    "Wistar rat"
  ],
  "suggested_hubs": [
    {
      "slug": "occupational-exposure",
      "weight": 0.2,
      "reason": "Study examines 60 Hz ELF magnetic field exposure, relevant to common power-frequency exposure contexts."
    }
  ]
}

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AI-extracted fields are generated from the abstract and metadata and may be incomplete or incorrect. This material is for informational purposes and is not medical advice.

Identifiers and source

DOI: 10.1186/s40200-014-0085-2
PubMed: 25152870

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