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Effects of 50 Hz electromagnetic fields on rat cortical synaptosomes.

Toxicology and industrial health · 2009 · No effect · Low evidence

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No effectLow evidencePaper 4946

After 2-hour exposure to a pulsed electromagnetic field with peak magnetic field 2 mT, O2 consumption and ATP production were unchanged. No synaptosomal membrane depolarization was detected; intrasynaptosomal Ca2+ concentration decreased slowly. Release of fr…

Study snapshot

PublicationToxicology and industrial health · 2009
Study typein_vitro
Reported directionNo effect
Evidence ratingLow

Abstract

Nerve cells are very responsive to weak pulsed electromagnetic fields (EMFs). Such non-ionizing radiation, with frequencies of 0-300 Hz and 0.1-100 mT, can affect several cellular activities, with unusual dose-response characteristics. The present study examined the effect of a 2-h exposure of synaptosomes on a system generating a peak magnetic field of 2 mT. We evaluated the changes of the synaptosomal mitochondrial respiration rate and ATP production, membrane potential, intrasynaptosomal Ca2+ concentration, and the release of free iron and F2-isoprostanes. O2 consumption and ATP production remained unchanged in exposed synaptosomes. The intrasynaptosomal Ca2+ concentration decreased slowly and no depolarization of the synaptosomal membrane was detected. Finally, the release of free iron and F2-isoprostanes by synaptosomal suspensions also remained unchanged after EMF exposure. These results indicate that the physiological behavior of cortical synaptosomes was unaffected by weak pulsed EMFs.

Machine Enhanced Logic

AI evidence extraction

Structured fields below were generated from the paper’s abstract and metadata to make the evidence easier to inspect. Automated extraction may be incomplete or incorrect; verify important details against the original paper.

Study typein_vitro
Effect directionno_effect
PopulationRat cortical synaptosomes (in vitro)
Sample sizeNot supplied
ExposureELF · 2 h
Evidence strengthLowConfidence: 74% · Peer reviewed: yes

Main findings

After 2-hour exposure to a pulsed electromagnetic field with peak magnetic field 2 mT, O2 consumption and ATP production were unchanged. No synaptosomal membrane depolarization was detected; intrasynaptosomal Ca2+ concentration decreased slowly. Release of free iron and F2-isoprostanes remained unchanged, and overall physiological behavior of cortical synaptosomes was reported as unaffected.

Outcomes measured

  • Synaptosomal mitochondrial respiration rate (O2 consumption)
  • ATP production
  • Synaptosomal membrane potential
  • Intrasynaptosomal Ca2+ concentration
  • Release of free iron
  • Release of F2-isoprostanes

Limitations

  • Sample size not reported in abstract
  • Exposure frequency not specified in abstract (paper title indicates 50 Hz, but abstract describes weak pulsed EMFs without stating frequency)
  • In vitro synaptosome preparation; may not generalize to in vivo outcomes
  • Short exposure duration (2 h)
View raw extracted JSON
{
  "study_type": "in_vitro",
  "exposure": {
    "band": "ELF",
    "source": null,
    "frequency_mhz": null,
    "sar_wkg": null,
    "duration": "2 h"
  },
  "population": "Rat cortical synaptosomes (in vitro)",
  "sample_size": null,
  "outcomes": [
    "Synaptosomal mitochondrial respiration rate (O2 consumption)",
    "ATP production",
    "Synaptosomal membrane potential",
    "Intrasynaptosomal Ca2+ concentration",
    "Release of free iron",
    "Release of F2-isoprostanes"
  ],
  "main_findings": "After 2-hour exposure to a pulsed electromagnetic field with peak magnetic field 2 mT, O2 consumption and ATP production were unchanged. No synaptosomal membrane depolarization was detected; intrasynaptosomal Ca2+ concentration decreased slowly. Release of free iron and F2-isoprostanes remained unchanged, and overall physiological behavior of cortical synaptosomes was reported as unaffected.",
  "effect_direction": "no_effect",
  "limitations": [
    "Sample size not reported in abstract",
    "Exposure frequency not specified in abstract (paper title indicates 50 Hz, but abstract describes weak pulsed EMFs without stating frequency)",
    "In vitro synaptosome preparation; may not generalize to in vivo outcomes",
    "Short exposure duration (2 h)"
  ],
  "evidence_strength": "low",
  "confidence": 0.74,
  "peer_reviewed_likely": "yes",
  "keywords": [
    "50 Hz",
    "ELF-EMF",
    "pulsed electromagnetic fields",
    "2 mT",
    "synaptosomes",
    "rat cortex",
    "mitochondrial respiration",
    "ATP",
    "membrane potential",
    "calcium",
    "free iron",
    "F2-isoprostanes",
    "oxidative stress"
  ],
  "suggested_hubs": []
}
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AI-extracted fields are generated from the abstract and metadata and may be incomplete or incorrect. This material is for informational purposes and is not medical advice.

Identifiers and source

DOI: 10.1177/0748233709103031
PubMed: 19651794

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