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Pulsed electromagnetic field (PEMF) prevents pro-oxidant effects of H2O2 in SK-N-BE(2) human neuroblastoma cells.

International journal of radiation biology · 2016 · Benefit · Low evidence

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BenefitLow evidencePaper 4875

In SK-N-BE(2) cells challenged with 1 mM hydrogen peroxide, repeated exposure to a 2 mT, 75 Hz PEMF increased MnSOD-based antioxidant protection and reduced ROS production compared with sham-exposed controls.

Study snapshot

PublicationInternational journal of radiation biology · 2016
Study typein_vitro
Reported directionBenefit
Evidence ratingLow

Abstract

Purpose The redox milieu, together with reactive oxygen species (ROS) accumulation, may play a role in mediating some biological effects of extremely-low-frequency electromagnetic fields (ELF-EMF). Some of us have recently reported that a pulsed EMF (PEMF) improves the antioxidant response of a drug-sensitive human neuroblastoma SH-SY5Y cell line to pro-oxidants. Since drug resistance may affect cell sensitivity to redox-based treatments, we wanted to verify whether drug-resistant human neuroblastoma SK-N-BE(2) cells respond to a PEMF in a similar fashion. Materials and methods SK-N-BE(2) cells were exposed to repeated 2 mT, 75 Hz PEMF (15 min each, repeated 3 times over 5 days), and ROS production, Mn-dependent superoxide dismutase (MnSOD)-based antioxidant protection and viability were assessed after 10 min or 30 min 1 mM hydrogen peroxide. Sham controls were kept at the same time in identical cell culture incubators. Results The PEMF increased the MnSOD-based antioxidant protection and reduced the ROS production in response to a pro-oxidant challenge. Conclusions Our work might lay foundation for the development of non-invasive PEMF-based approaches aimed at elevating endogenous antioxidant properties in cellular or tissue models.

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AI evidence extraction

Structured fields below were generated from the paper’s abstract and metadata to make the evidence easier to inspect. Automated extraction may be incomplete or incorrect; verify important details against the original paper.

Study typein_vitro
Effect directionbenefit
PopulationSK-N-BE(2) human neuroblastoma cells (drug-resistant)
Sample sizeNot supplied
ExposureELF · 15 min each exposure, repeated 3 times over 5 days
Evidence strengthLowConfidence: 78% · Peer reviewed: yes

Main findings

In SK-N-BE(2) cells challenged with 1 mM hydrogen peroxide, repeated exposure to a 2 mT, 75 Hz PEMF increased MnSOD-based antioxidant protection and reduced ROS production compared with sham-exposed controls.

Outcomes measured

  • ROS production
  • Mn-dependent superoxide dismutase (MnSOD)-based antioxidant protection
  • cell viability (after H2O2 challenge)

Limitations

  • In vitro cell culture model; generalizability to humans or tissues not established in abstract
  • Sample size and replication details not reported in abstract
  • Outcomes assessed in the context of an acute pro-oxidant (H2O2) challenge; effects without challenge not described
View raw extracted JSON
{
  "study_type": "in_vitro",
  "exposure": {
    "band": "ELF",
    "source": "other",
    "frequency_mhz": null,
    "sar_wkg": null,
    "duration": "15 min each exposure, repeated 3 times over 5 days"
  },
  "population": "SK-N-BE(2) human neuroblastoma cells (drug-resistant)",
  "sample_size": null,
  "outcomes": [
    "ROS production",
    "Mn-dependent superoxide dismutase (MnSOD)-based antioxidant protection",
    "cell viability (after H2O2 challenge)"
  ],
  "main_findings": "In SK-N-BE(2) cells challenged with 1 mM hydrogen peroxide, repeated exposure to a 2 mT, 75 Hz PEMF increased MnSOD-based antioxidant protection and reduced ROS production compared with sham-exposed controls.",
  "effect_direction": "benefit",
  "limitations": [
    "In vitro cell culture model; generalizability to humans or tissues not established in abstract",
    "Sample size and replication details not reported in abstract",
    "Outcomes assessed in the context of an acute pro-oxidant (H2O2) challenge; effects without challenge not described"
  ],
  "evidence_strength": "low",
  "confidence": 0.78,
  "peer_reviewed_likely": "yes",
  "keywords": [
    "PEMF",
    "ELF-EMF",
    "75 Hz",
    "2 mT",
    "oxidative stress",
    "ROS",
    "MnSOD",
    "hydrogen peroxide",
    "neuroblastoma",
    "SK-N-BE(2)",
    "antioxidant response"
  ],
  "suggested_hubs": []
}
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AI-extracted fields are generated from the abstract and metadata and may be incomplete or incorrect. This material is for informational purposes and is not medical advice.

Identifiers and source

DOI: 10.3109/09553002.2016.1150619
PubMed: 26940444

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