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Short-term Exposure to 50-Hz Electromagnetic Field and Alterations in and Expression in MCF-7 Cells.

Open access Macedonian journal of medical sciences · 2016 · Mixed · Very low evidence

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MixedVery low evidencePaper 4808

NQO1 mRNA level (0h) decreased after exposure to 0.25 mT under the 5 min field-on/5 min field-off pattern compared with unexposed cells. HO-1 mRNA level increased (0h and 2h) after exposure to 0.50 mT under the 5 min field-on/5 min field-off pattern compared …

Study snapshot

PublicationOpen access Macedonian journal of medical sciences · 2016
Study typein_vitro
Reported directionMixed
Evidence ratingVery low

Abstract

AIM: Extremely low-frequency electromagnetic fields (ELF-EMFs) have some genotoxic effects and it may alter the mRNA levels of antioxidant genes. The NAD(P)H: quinone oxidoreductase-1 () and are ubiquitously expressed. Considering that there is no published data on the effect(s) of ELF-EMF (50-Hz) exposure and expression levels of and in the human MCF-7 cells, the present study was carried out. METHODS: The ELF-EMF (0.25 and 0.50 mT) exposure patterns were: 5 min field-on/5 min filed-off, 15 min field-on/15 min field-off, and 30 min field-on continuously. In all exposure conditions, total exposure time were 30 minutes. The RNA extraction was done at two times; immediately post exposure and two hours post exposure. The effect of ELF-EMF on gene expression was assessed by real-time PCR. RESULTS: The mRNA level (at 0h) decreased in the cells exposed to 5 min field-on/5 min filed-off condition at 0.25 mT EMF when compared with the unexposed cells. The mRNA level (at 0h and 2h) increased in the cells exposed to 5 min field-on/5 min filed-off condition at 0.50 mT EMF when compared with the unexposed cells. CONCLUSIONS: Alterations in the and mRNA levels seem at the "5 min field-on/5 min field-off" condition.

Machine Enhanced Logic

AI evidence extraction

Structured fields below were generated from the paper’s abstract and metadata to make the evidence easier to inspect. Automated extraction may be incomplete or incorrect; verify important details against the original paper.

Study typein_vitro
Effect directionmixed
PopulationHuman MCF-7 cells
Sample sizeNot supplied
ExposureELF · 0.05 MHz · 30 minutes total (patterns: 5 min on/5 min off; 15 min on/15 min off; 30 min continuous); RNA measured immediately and 2 hours post-exposure
Evidence strengthVery lowConfidence: 74% · Peer reviewed: yes

Main findings

NQO1 mRNA level (0h) decreased after exposure to 0.25 mT under the 5 min field-on/5 min field-off pattern compared with unexposed cells. HO-1 mRNA level increased (0h and 2h) after exposure to 0.50 mT under the 5 min field-on/5 min field-off pattern compared with unexposed cells.

Outcomes measured

  • mRNA expression levels of NQO1
  • mRNA expression levels of HO-1

Limitations

  • Sample size not reported in abstract
  • Only short-term exposure (30 minutes) assessed
  • Only one cell line (MCF-7) studied
  • Limited outcomes (two antioxidant-related genes)
  • Results highlighted for one intermittent exposure pattern; effects for other patterns not described in results
View raw extracted JSON
{
  "study_type": "in_vitro",
  "exposure": {
    "band": "ELF",
    "source": null,
    "frequency_mhz": 0.05,
    "sar_wkg": null,
    "duration": "30 minutes total (patterns: 5 min on/5 min off; 15 min on/15 min off; 30 min continuous); RNA measured immediately and 2 hours post-exposure"
  },
  "population": "Human MCF-7 cells",
  "sample_size": null,
  "outcomes": [
    "mRNA expression levels of NQO1",
    "mRNA expression levels of HO-1"
  ],
  "main_findings": "NQO1 mRNA level (0h) decreased after exposure to 0.25 mT under the 5 min field-on/5 min field-off pattern compared with unexposed cells. HO-1 mRNA level increased (0h and 2h) after exposure to 0.50 mT under the 5 min field-on/5 min field-off pattern compared with unexposed cells.",
  "effect_direction": "mixed",
  "limitations": [
    "Sample size not reported in abstract",
    "Only short-term exposure (30 minutes) assessed",
    "Only one cell line (MCF-7) studied",
    "Limited outcomes (two antioxidant-related genes)",
    "Results highlighted for one intermittent exposure pattern; effects for other patterns not described in results"
  ],
  "evidence_strength": "very_low",
  "confidence": 0.74,
  "peer_reviewed_likely": "yes",
  "keywords": [
    "ELF-EMF",
    "50 Hz",
    "MCF-7",
    "gene expression",
    "mRNA",
    "NQO1",
    "HO-1",
    "antioxidant genes",
    "real-time PCR",
    "intermittent exposure",
    "0.25 mT",
    "0.50 mT"
  ],
  "suggested_hubs": []
}
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AI-extracted fields are generated from the abstract and metadata and may be incomplete or incorrect. This material is for informational purposes and is not medical advice.

Identifiers and source

DOI: 10.3889/oamjms.2016.102
PubMed: 28028389

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