Effects of extremely low-frequency pulsed electromagnetic fields on morphological and biochemical properties of human breast carcinoma cells (T47D).

Electromagnetic biology and medicine · 2012 · Mixed · Low evidence

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MixedLow evidencePaper 4521

Study snapshot

PublicationElectromagnetic biology and medicine · 2012

Study typein_vitro

Reported directionMixed

Evidence ratingLow

Abstract

This study was carried out to investigate the effects of 100 and 217 Hz extremely low-frequency pulsed electromagnetic fields (ELF-PEMF) on cell proliferation, actin reorganization, and ROS generation in a human breast carcinoma cells (T47D). Cells were exposed for 24-72 h, at 100 and 217 Hz, 0.1 mT. The treatment induced a time dependent decrease in cell growth after 72 h and revealed an increase in fluorescence intensity in cytoplasm and actin aggregations around the nucleus as detected by fluorescence microscopy. The amount of actin in T47D cells increased after 48 h exposure to 100 Hz and 24 h to 217 Hz while no changes in nuclear morphology were detected. Exposing the cells to 217 Hz for 72 h caused a dramatically increase of intracellular ROS generation while with exposure to 100 Hz it remained nearly unchanged. These results suggest that exposure to ELF-PEMF (100, 217 Hz, 0.1 mT) are able inducing an increase of actin level, its migration toward nucleus but despite of these changes and dramatically increase in ROS generation the symptoms of apoptosis were not observed. Our results support the hypothesis that cell response to EMF may only be observed at certain window effects; such as frequency and intensity of EMF parameters.

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AI evidence extraction

Structured fields below were generated from the paper’s abstract and metadata to make the evidence easier to inspect. Automated extraction may be incomplete or incorrect; verify important details against the original paper.

Study typein_vitro

Effect directionmixed

PopulationHuman breast carcinoma cell line (T47D)

Sample sizeNot supplied

ExposureELF · 24–72 h; 100 or 217 Hz; 0.1 mT

Evidence strengthLowConfidence: 78% · Peer reviewed: yes

Main findings

T47D cells exposed to ELF-PEMF (0.1 mT) showed a time-dependent decrease in cell growth after 72 h. Actin fluorescence/aggregation around the nucleus increased, with actin amount increased after 48 h at 100 Hz and after 24 h at 217 Hz; nuclear morphology did not change. ROS generation increased dramatically after 72 h at 217 Hz but remained nearly unchanged at 100 Hz, and symptoms of apoptosis were not observed.

Outcomes measured

Cell proliferation/growth
Actin reorganization and actin level
Nuclear morphology
Intracellular reactive oxygen species (ROS) generation
Apoptosis (symptoms)

View raw extracted JSON

{
  "study_type": "in_vitro",
  "exposure": {
    "band": "ELF",
    "source": "pulsed electromagnetic fields (PEMF)",
    "frequency_mhz": null,
    "sar_wkg": null,
    "duration": "24–72 h; 100 or 217 Hz; 0.1 mT"
  },
  "population": "Human breast carcinoma cell line (T47D)",
  "sample_size": null,
  "outcomes": [
    "Cell proliferation/growth",
    "Actin reorganization and actin level",
    "Nuclear morphology",
    "Intracellular reactive oxygen species (ROS) generation",
    "Apoptosis (symptoms)"
  ],
  "main_findings": "T47D cells exposed to ELF-PEMF (0.1 mT) showed a time-dependent decrease in cell growth after 72 h. Actin fluorescence/aggregation around the nucleus increased, with actin amount increased after 48 h at 100 Hz and after 24 h at 217 Hz; nuclear morphology did not change. ROS generation increased dramatically after 72 h at 217 Hz but remained nearly unchanged at 100 Hz, and symptoms of apoptosis were not observed.",
  "effect_direction": "mixed",
  "limitations": [],
  "evidence_strength": "low",
  "confidence": 0.78,
  "peer_reviewed_likely": "yes",
  "keywords": [
    "extremely low-frequency",
    "ELF",
    "pulsed electromagnetic fields",
    "PEMF",
    "100 Hz",
    "217 Hz",
    "0.1 mT",
    "T47D",
    "breast carcinoma cells",
    "cell proliferation",
    "actin reorganization",
    "ROS"
  ],
  "suggested_hubs": []
}

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AI-extracted fields are generated from the abstract and metadata and may be incomplete or incorrect. This material is for informational purposes and is not medical advice.

Identifiers and source

DOI: 10.3109/15368378.2012.683844
PubMed: 22676212

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