Exposure to the electromagnetic field alters the transcriptomic profile in the porcine endometrium during the peri-implantation period

2021 · Harm · Low evidence

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HarmLow evidencePaper 853

Study snapshot

Publicationmanual · 2021

Study typein_vitro

Reported directionHarm

Evidence ratingLow

Abstract

Exposure to the electromagnetic field alters the transcriptomic profile in the porcine endometrium during the peri-implantation period Kozlowska W, Drzewiecka EM, Paukszto L, Zmijewska A, Wydorski PJ, Jastrzebski JP, Franczak A. Exposure to the electromagnetic field alters the transcriptomic profile in the porcine endometrium during the peri-implantation period. J Physiol Pharmacol. 2021 Dec;72(6). doi: 10.26402/jpp.2021.6.04. Abstract A low-frequency electromagnetic field (EMF) is an environmental pollutant that may influence female reproduction. This research was undertaken to test the hypothesis that EMF causes alterations in the transcriptomic profile of the endometrium. This study investigated the in vitro effects of EMF treatment (50 Hz, 2 h) on global transcriptome alterations in the endometrium isolated from pigs during the peri-implantation period. The control endometrium was not treated with EMF. The EMF treatment altered the expression of 1561 transcriptionally active regions (TARs) in the endometrium. In the group of 461 evaluated DEGs, 156 were up- regulated (34%), 305 were down-regulated (66%) and 341 (74%) had known biological functions. A total of 210 long noncoding RNAs (lncRNAs) with changes in expression profiles, and 146 predicted RNA editing sites were also evaluated. Exposure to EMF changes the expression of genes encoding proteins that are involved in proliferation and metabolism in endometrial tissue. These results provide useful inputs for further research into the impact of EMF on molecular changes in the uterus during the peri-implantation period and, consequently, pregnancy outcome. Open access paper: jpp.krakow.pl

Machine Enhanced Logic

AI evidence extraction

Structured fields below were generated from the paper’s abstract and metadata to make the evidence easier to inspect. Automated extraction may be incomplete or incorrect; verify important details against the original paper.

Study typein_vitro

Effect directionharm

PopulationPorcine endometrium (peri-implantation period), isolated tissue (in vitro)

Sample sizeNot supplied

ExposureELF · 2 h

Evidence strengthLowConfidence: 78% · Peer reviewed: yes

Main findings

In vitro exposure of porcine endometrium to a low-frequency EMF (50 Hz) for 2 hours altered expression of 1561 transcriptionally active regions. Among 461 evaluated differentially expressed genes, 156 were up-regulated and 305 down-regulated; 210 lncRNAs with altered expression and 146 predicted RNA editing sites were also reported. The altered genes included those involved in proliferation and metabolism.

Outcomes measured

Global transcriptome alterations (TARs/DEGs)
lncRNA expression profile changes
Predicted RNA editing sites
Gene expression related to proliferation and metabolism

Limitations

In vitro study using isolated porcine endometrium; may not reflect in vivo reproductive outcomes
No pregnancy or implantation outcomes measured; molecular endpoints only
Sample size not reported in the provided abstract
Exposure characterization limited to frequency and duration (field strength not provided)

View raw extracted JSON

{
  "study_type": "in_vitro",
  "exposure": {
    "band": "ELF",
    "source": null,
    "frequency_mhz": null,
    "sar_wkg": null,
    "duration": "2 h"
  },
  "population": "Porcine endometrium (peri-implantation period), isolated tissue (in vitro)",
  "sample_size": null,
  "outcomes": [
    "Global transcriptome alterations (TARs/DEGs)",
    "lncRNA expression profile changes",
    "Predicted RNA editing sites",
    "Gene expression related to proliferation and metabolism"
  ],
  "main_findings": "In vitro exposure of porcine endometrium to a low-frequency EMF (50 Hz) for 2 hours altered expression of 1561 transcriptionally active regions. Among 461 evaluated differentially expressed genes, 156 were up-regulated and 305 down-regulated; 210 lncRNAs with altered expression and 146 predicted RNA editing sites were also reported. The altered genes included those involved in proliferation and metabolism.",
  "effect_direction": "harm",
  "limitations": [
    "In vitro study using isolated porcine endometrium; may not reflect in vivo reproductive outcomes",
    "No pregnancy or implantation outcomes measured; molecular endpoints only",
    "Sample size not reported in the provided abstract",
    "Exposure characterization limited to frequency and duration (field strength not provided)"
  ],
  "evidence_strength": "low",
  "confidence": 0.78,
  "peer_reviewed_likely": "yes",
  "keywords": [
    "extremely low frequency",
    "ELF EMF",
    "50 Hz",
    "endometrium",
    "porcine",
    "peri-implantation",
    "transcriptome",
    "differential gene expression",
    "lncRNA",
    "RNA editing",
    "proliferation",
    "metabolism"
  ],
  "suggested_hubs": [
    {
      "slug": "elF-emf",
      "weight": 0.7,
      "reason": "Study uses low-frequency (50 Hz) electromagnetic field exposure."
    },
    {
      "slug": "reproductive-health",
      "weight": 0.6,
      "reason": "Focus on endometrium during peri-implantation period and implications for pregnancy outcomes."
    }
  ]
}

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AI-extracted fields are generated from the abstract and metadata and may be incomplete or incorrect. This material is for informational purposes and is not medical advice.

Identifiers and source

DOI: 10.26402/jpp.2021.6.04
PubMed: Not supplied

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