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Intracellular Ca Mobilization and Beta-hexosaminidase Release Are Not Influenced by 60 Hz-electromagnetic Fields (EMF) in RBL 2H3 Cells.

The Korean journal of physiology & pharmacology : official journal of the Korean Physiological Society and the Korean Society of Pharmacology · 2011 · No effect · Low evidence

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No effectLow evidencePaper 4429

In RBL 2H3 cells, exposure to 60 Hz EMF at 0.1 or 1 mT for 4 or 16 hours produced no cytotoxic effects and did not affect intracellular Ca(2+) increases induced by melittin, ionomycin, or thapsigargin. The same EMF exposures did not affect basal or 1 µM melit…

Study snapshot

PublicationThe Korean journal of physiology & pharmacology : official journal of the Korean Physiological Society and the Korean Society of Pharmacology · 2011
Study typein_vitro
Reported directionNo effect
Evidence ratingLow

Abstract

The effects of extremely low frequency electromagnetic fields (EMF) on intracellular Ca(2+) mobilization and cellular function in RBL 2H3 cells were investigated. Exposure to EMF (60 Hz, 0.1 or 1 mT) for 4 or 16 h did not produce any cytotoxic effects in RBL 2H3 cells. Melittin, ionomycin and thapsigargin each dose-dependently increased the intracellular Ca(2+) concentration. The increase of intracellular Ca(2+) induced by these three agents was not affected by exposure to EMF (60 Hz, 1 mT) for 4 or 16 h in RBL 2H3 cells. To investigate the effect of EMF on exocytosis, we measured beta-hexosaminidase release in RBL 2H3 cells. Basal release of beta-hexosaminidase was 12.3±2.3% in RBL 2H3 cells. Exposure to EMF (60 Hz, 0.1 or 1 mT) for 4 or 16 h did not affect the basal or 1 µM melittin-induced beta-hexosaminidase release in RBL 2H3 cells. This study suggests that exposure to EMF (60 Hz, 0.1 or 1 mT), which is the limit of occupational exposure, has no influence on intracellular Ca(2+) mobilization and cellular function in RBL 2H3 cells.

Machine Enhanced Logic

AI evidence extraction

Structured fields below were generated from the paper’s abstract and metadata to make the evidence easier to inspect. Automated extraction may be incomplete or incorrect; verify important details against the original paper.

Study typein_vitro
Effect directionno_effect
PopulationRBL 2H3 cells
Sample sizeNot supplied
ExposureELF · 6e-05 MHz · 4 or 16 h
Evidence strengthLowConfidence: 78% · Peer reviewed: yes

Main findings

In RBL 2H3 cells, exposure to 60 Hz EMF at 0.1 or 1 mT for 4 or 16 hours produced no cytotoxic effects and did not affect intracellular Ca(2+) increases induced by melittin, ionomycin, or thapsigargin. The same EMF exposures did not affect basal or 1 µM melittin-induced beta-hexosaminidase release.

Outcomes measured

  • Intracellular Ca(2+) mobilization (intracellular Ca(2+) concentration)
  • Cytotoxicity
  • Exocytosis / beta-hexosaminidase release (basal and melittin-induced)
View raw extracted JSON
{
  "study_type": "in_vitro",
  "exposure": {
    "band": "ELF",
    "source": "other",
    "frequency_mhz": 6e-05,
    "sar_wkg": null,
    "duration": "4 or 16 h"
  },
  "population": "RBL 2H3 cells",
  "sample_size": null,
  "outcomes": [
    "Intracellular Ca(2+) mobilization (intracellular Ca(2+) concentration)",
    "Cytotoxicity",
    "Exocytosis / beta-hexosaminidase release (basal and melittin-induced)"
  ],
  "main_findings": "In RBL 2H3 cells, exposure to 60 Hz EMF at 0.1 or 1 mT for 4 or 16 hours produced no cytotoxic effects and did not affect intracellular Ca(2+) increases induced by melittin, ionomycin, or thapsigargin. The same EMF exposures did not affect basal or 1 µM melittin-induced beta-hexosaminidase release.",
  "effect_direction": "no_effect",
  "limitations": [],
  "evidence_strength": "low",
  "confidence": 0.78,
  "peer_reviewed_likely": "yes",
  "keywords": [
    "extremely low frequency",
    "ELF",
    "electromagnetic fields",
    "60 Hz",
    "RBL 2H3",
    "calcium mobilization",
    "Ca2+",
    "beta-hexosaminidase",
    "exocytosis",
    "melittin",
    "ionomycin",
    "thapsigargin",
    "occupational exposure limit"
  ],
  "suggested_hubs": [
    {
      "slug": "occupational-exposure",
      "weight": 0.62,
      "reason": "Exposure levels described as the limit of occupational exposure (60 Hz, 0.1 or 1 mT)."
    }
  ]
}
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AI-extracted fields are generated from the abstract and metadata and may be incomplete or incorrect. This material is for informational purposes and is not medical advice.

Identifiers and source

DOI: 10.4196/kjpp.2011.15.5.313
PubMed: 22128265

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